On-line Detection of Acetate Formation in Escherichia coli Cultures using Dissolved Oxygen Responses to Feed Transients
Åkesson, Mats ; Nordberg Karlsson, Eva LU
; Hagander, Per
LU
; Axelsson, Jan Peter
and Tocaj, Anita
(1999)
In Biotechnology and Bioengineering
64(5).
p.590-598
- Abstract
- Recombinant protein production in <i>Escherichia coli</i> can be significantly reduced by acetate accumulation. It is demonstrated that acetate production can be detected on-line with a standard dissolved oxygen sensor by superimposing short pulses to the substrate feed rate. Assuming that acetate formation is linked to a respiratory limitation, a model for dissolved oxygen responses to transients in substrate feed rate is derived. The model predicts a clear change in the character of the transient response when acetate formation starts. The predicted effect was verified in fed-batch cultivations of E. coli TOPP1 and E. coli BL21(DE3), both before and after induction of recombinant protein production. It was also observed that... (More)
- Recombinant protein production in <i>Escherichia coli</i> can be significantly reduced by acetate accumulation. It is demonstrated that acetate production can be detected on-line with a standard dissolved oxygen sensor by superimposing short pulses to the substrate feed rate. Assuming that acetate formation is linked to a respiratory limitation, a model for dissolved oxygen responses to transients in substrate feed rate is derived. The model predicts a clear change in the character of the transient response when acetate formation starts. The predicted effect was verified in fed-batch cultivations of E. coli TOPP1 and E. coli BL21(DE3), both before and after induction of recombinant protein production. It was also observed that the critical specific glucose uptake rate, at which acetate formation starts, was significantly decreased after induction. On-line detection of acetate formation with a standard sensor opens up new possibilities for feedback control of substrate feeding. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2174874
- author
- Åkesson, Mats
; Nordberg Karlsson, Eva
LU
; Hagander, Per
LU
; Axelsson, Jan Peter
and Tocaj, Anita
- organization
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- on-line detection, acetate, \emph{Escherichia coli}, dissolved oxygen, transients, mathematical model
- in
- Biotechnology and Bioengineering
- volume
- 64
- issue
- 5
- pages
- 590 - 598
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:0345085733
- ISSN
- 1097-0290
- DOI
- 10.1002/(SICI)1097-0290(19990905)64:5<590::AID-BIT9>3.0.CO;2-T/abstract
- language
- English
- LU publication?
- yes
- additional info
- key=ake+99bb
- id
- de6fcbe2-79c1-4731-8e3b-f7599b507cee (old id 2174874)
- date added to LUP
- 2016-04-01 12:22:08
- date last changed
- 2022-01-27 02:48:38
@article{de6fcbe2-79c1-4731-8e3b-f7599b507cee,
abstract = {{Recombinant protein production in <i>Escherichia coli</i> can be significantly reduced by acetate accumulation. It is demonstrated that acetate production can be detected on-line with a standard dissolved oxygen sensor by superimposing short pulses to the substrate feed rate. Assuming that acetate formation is linked to a respiratory limitation, a model for dissolved oxygen responses to transients in substrate feed rate is derived. The model predicts a clear change in the character of the transient response when acetate formation starts. The predicted effect was verified in fed-batch cultivations of E. coli TOPP1 and E. coli BL21(DE3), both before and after induction of recombinant protein production. It was also observed that the critical specific glucose uptake rate, at which acetate formation starts, was significantly decreased after induction. On-line detection of acetate formation with a standard sensor opens up new possibilities for feedback control of substrate feeding.}},
author = {{Åkesson, Mats and Nordberg Karlsson, Eva and Hagander, Per and Axelsson, Jan Peter and Tocaj, Anita}},
issn = {{1097-0290}},
keywords = {{on-line detection; acetate; \emph{Escherichia coli}; dissolved oxygen; transients; mathematical model}},
language = {{eng}},
number = {{5}},
pages = {{590--598}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Biotechnology and Bioengineering}},
title = {{On-line Detection of Acetate Formation in Escherichia coli Cultures using Dissolved Oxygen Responses to Feed Transients}},
url = {{http://dx.doi.org/10.1002/(SICI)1097-0290(19990905)64:5<590::AID-BIT9>3.0.CO;2-T/abstract}},
doi = {{10.1002/(SICI)1097-0290(19990905)64:5<590::AID-BIT9>3.0.CO;2-T/abstract}},
volume = {{64}},
year = {{1999}},
}