Actin-like protein associated with plasma membranes from Euglena gracilis
(1998) In Protoplasma 202(3-4). p.153-160- Abstract
- Microtubules are characteristic components of the membrane skeleton of Euglena gracilis, but whether microfilaments are present has been controversial. We here present evidence that an actin-like protein may indeed be associated with the plasma membrane (PM) of E. gracilis. Firstly, a 47 kDa, PM-associated, polypeptide was recognized by an anti-amoeba actin antibody. Secondly, this 47 kDa protein seemed to be peripherally attached to PM in much the same way as β-tubulin, since both could be released from PM by treatment with 150 mM NaOH but not with ethylene glycol, NaCl, or formamide. Thirdly, the 47 kDa polypeptide and β-tubulin were found mainly in the Triton X-1 14-insoluble fraction, indicating that they were part of a protein complex... (More)
- Microtubules are characteristic components of the membrane skeleton of Euglena gracilis, but whether microfilaments are present has been controversial. We here present evidence that an actin-like protein may indeed be associated with the plasma membrane (PM) of E. gracilis. Firstly, a 47 kDa, PM-associated, polypeptide was recognized by an anti-amoeba actin antibody. Secondly, this 47 kDa protein seemed to be peripherally attached to PM in much the same way as β-tubulin, since both could be released from PM by treatment with 150 mM NaOH but not with ethylene glycol, NaCl, or formamide. Thirdly, the 47 kDa polypeptide and β-tubulin were found mainly in the Triton X-1 14-insoluble fraction, indicating that they were part of a protein complex resistant to detergents, such as the cytoskeleton. Finally, DNase I activity was inhibited by a fraction enriched in the 47 kDa polypeptide, a property typical of actin. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4438048
- author
- Petersen-Mahrt, Silja ; Sonesson, Anders LU and Widell, Susanne LU
- organization
- publishing date
- 1998
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Actin, Anti-actin antibody, Cytoskeleton, Euglena gracilis, Triton X-114, DNAse I
- in
- Protoplasma
- volume
- 202
- issue
- 3-4
- pages
- 8 pages
- publisher
- Springer
- external identifiers
-
- scopus:0031839279
- ISSN
- 1615-6102
- DOI
- 10.1007/BF01282543
- language
- English
- LU publication?
- yes
- additional info
- Department affilation moved from v1000887 (CED - Centre for Educational Development) to v1000942 (Division for Higher Education Development) on 2016-03-31 08:48:47.
- id
- c80c4c40-206a-4a63-a408-de39707c7cf7 (old id 4438048)
- date added to LUP
- 2016-04-04 08:12:36
- date last changed
- 2023-01-05 08:09:16
@article{c80c4c40-206a-4a63-a408-de39707c7cf7, abstract = {{Microtubules are characteristic components of the membrane skeleton of Euglena gracilis, but whether microfilaments are present has been controversial. We here present evidence that an actin-like protein may indeed be associated with the plasma membrane (PM) of E. gracilis. Firstly, a 47 kDa, PM-associated, polypeptide was recognized by an anti-amoeba actin antibody. Secondly, this 47 kDa protein seemed to be peripherally attached to PM in much the same way as β-tubulin, since both could be released from PM by treatment with 150 mM NaOH but not with ethylene glycol, NaCl, or formamide. Thirdly, the 47 kDa polypeptide and β-tubulin were found mainly in the Triton X-1 14-insoluble fraction, indicating that they were part of a protein complex resistant to detergents, such as the cytoskeleton. Finally, DNase I activity was inhibited by a fraction enriched in the 47 kDa polypeptide, a property typical of actin.}}, author = {{Petersen-Mahrt, Silja and Sonesson, Anders and Widell, Susanne}}, issn = {{1615-6102}}, keywords = {{Actin; Anti-actin antibody; Cytoskeleton; Euglena gracilis; Triton X-114; DNAse I}}, language = {{eng}}, number = {{3-4}}, pages = {{153--160}}, publisher = {{Springer}}, series = {{Protoplasma}}, title = {{Actin-like protein associated with plasma membranes from Euglena gracilis}}, url = {{http://dx.doi.org/10.1007/BF01282543}}, doi = {{10.1007/BF01282543}}, volume = {{202}}, year = {{1998}}, }