Upscaling of novel probiotics production and evaluation of ultraviolet (UV) radiation resistance
(2025) KLTM02 20251Food Technology and Nutrition (M.Sc.)
- Abstract
- As traditional probiotic bacteria such as lactic acid bacteria (LAB) and bifidobacteria are sensitive to industrial processes, more robust spore forming bacteria has gained attention to be used as probiotics. The present project involves scaling up the production of Heyndrickxia sp. with the aim to achieve high yield and purity at each stage of the process. At the end, the harvested cells were freeze-dried for stability and storage of the strain. The upscaling process was successful with a yield of 7.6108 CFU/g after freeze-drying and with a sequence similarity above 95% with Heyndrickxia sp.
The field of plant probiotics is increasing in popularity to promote plant growth and optimize the microbiota, but hydroponic systems are often... (More) - As traditional probiotic bacteria such as lactic acid bacteria (LAB) and bifidobacteria are sensitive to industrial processes, more robust spore forming bacteria has gained attention to be used as probiotics. The present project involves scaling up the production of Heyndrickxia sp. with the aim to achieve high yield and purity at each stage of the process. At the end, the harvested cells were freeze-dried for stability and storage of the strain. The upscaling process was successful with a yield of 7.6108 CFU/g after freeze-drying and with a sequence similarity above 95% with Heyndrickxia sp.
The field of plant probiotics is increasing in popularity to promote plant growth and optimize the microbiota, but hydroponic systems are often disinfected by UV radiation, leading to survivability challenges. The study therefore evaluated the survivability of two strains when exposed to different doses of UV radiation. The exposure doses were set to as 60, 80, 100, 250 and 350 j/cm2, and the reduction in CFU/ml were compared in between each dose. There was a statistically significant reduction in the CFU/ml after exposure to 60 J/cm2 for both strains. The reduction in the concentration in between each dose (60 to 80, 80 to 100, 100 to 250 and 250 to 350 J/cm2) was not statistically significant based on the Mann–Whitney U test. The strains showed resistance to radiation doses up to 350 J/cm2 which is higher than the minimum required dose (250 mJ/cm2) for disinfection, indicating their potential suitability for application in hydroponics systems. (Less) - Popular Abstract
- Production and UV-C Resistance Evaluation of Heyndrickxia Species: A Promising New Probiotic for Health and Sustainable Agriculture.
Are all bacteria harmful? Absolutely not!. In fact, bacteria are essential to our health. Our bodies, especially our gut, are home to trillions of bacteria that help with digestion, immune function, and overall well-being. The beneficial ones are called “probiotics”, these are friendly bacteria that support a healthy balance in our bodies and can be added to our diet.
You can find probiotics in many everyday products like fruit juices, yogurt, milk-based drinks, chocolates, and even dietary supplements. The most common probiotic bacteria belong to groups like lactic acid bacteria (LAB) and... (More) - Production and UV-C Resistance Evaluation of Heyndrickxia Species: A Promising New Probiotic for Health and Sustainable Agriculture.
Are all bacteria harmful? Absolutely not!. In fact, bacteria are essential to our health. Our bodies, especially our gut, are home to trillions of bacteria that help with digestion, immune function, and overall well-being. The beneficial ones are called “probiotics”, these are friendly bacteria that support a healthy balance in our bodies and can be added to our diet.
You can find probiotics in many everyday products like fruit juices, yogurt, milk-based drinks, chocolates, and even dietary supplements. The most common probiotic bacteria belong to groups like lactic acid bacteria (LAB) and bifidobacteria. And it’s not just humans who benefit, probiotics are also helpful for animals and plants, improving their health and resilience in various environments.
In this study, we are working with two new probiotic bacteria (Heyndrickxia sp.), which not only benefit human health but also support plant growth. These strains are spore-forming bacteria, meaning they can survive harsh environmental conditions like high temperatures and low pH much better than traditional probiotics such as LAB and bifidobacteria.
A key part of our research focuses on scaling up the production of one of these strains to achieve high yield and purity, making it suitable for practical use. Another important goal is to evaluate the survivability of the two strains when exposed to UV-C light, which is commonly used to disinfect water in hydroponic systems, a soil-free method of growing plants in nutrient-rich water. Since disinfection is crucial to prevent plant diseases, we need to ensure the probiotic bacteria can survive UV treatment if they are to be used successfully in these systems.
This study highlights the potential of Heyndrickxia sp., as a new probiotic strain, not only for human health but also for sustainable agriculture. The successful integration of the strains into systems like hydroponics could open new doors for eco-friendly farming, improve plant health, and reduce reliance on chemical fertilizers. (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/9192776
- author
- Jose, Alphonse LU and Mathew, Shinu LU
- supervisor
-
- Åsa Håkansson LU
- Elisabeth Uhlig LU
- organization
- course
- KLTM02 20251
- year
- 2025
- type
- H2 - Master's Degree (Two Years)
- subject
- keywords
- Upscaling, probiotics, ultraviolet radiation, biotechnology
- language
- English
- id
- 9192776
- date added to LUP
- 2025-06-11 11:04:47
- date last changed
- 2025-06-11 11:04:47
@misc{9192776,
abstract = {{As traditional probiotic bacteria such as lactic acid bacteria (LAB) and bifidobacteria are sensitive to industrial processes, more robust spore forming bacteria has gained attention to be used as probiotics. The present project involves scaling up the production of Heyndrickxia sp. with the aim to achieve high yield and purity at each stage of the process. At the end, the harvested cells were freeze-dried for stability and storage of the strain. The upscaling process was successful with a yield of 7.6108 CFU/g after freeze-drying and with a sequence similarity above 95% with Heyndrickxia sp.
The field of plant probiotics is increasing in popularity to promote plant growth and optimize the microbiota, but hydroponic systems are often disinfected by UV radiation, leading to survivability challenges. The study therefore evaluated the survivability of two strains when exposed to different doses of UV radiation. The exposure doses were set to as 60, 80, 100, 250 and 350 j/cm2, and the reduction in CFU/ml were compared in between each dose. There was a statistically significant reduction in the CFU/ml after exposure to 60 J/cm2 for both strains. The reduction in the concentration in between each dose (60 to 80, 80 to 100, 100 to 250 and 250 to 350 J/cm2) was not statistically significant based on the Mann–Whitney U test. The strains showed resistance to radiation doses up to 350 J/cm2 which is higher than the minimum required dose (250 mJ/cm2) for disinfection, indicating their potential suitability for application in hydroponics systems.}},
author = {{Jose, Alphonse and Mathew, Shinu}},
language = {{eng}},
note = {{Student Paper}},
title = {{Upscaling of novel probiotics production and evaluation of ultraviolet (UV) radiation resistance}},
year = {{2025}},
}