Cold stress decreases the capacity for respiratory NADH oxidation in potato leaves.
(2002) In FEBS Letters 517(1-3). p.79-82- Abstract
- Cold stress effects on the expression of genes for respiratory chain enzymes were investigated in potato (Solanum tuberosum L., cv. Desiree) leaves. The nda1 and ndb1 genes, homologues to genes encoding the non-proton-pumping respiratory chain NADH dehydrogenases of Escherichia coli and yeast, were compared to genes encoding catalytic subunits of the proton-pumping NADH dehydrogenase (complex I). Using a real-time PCR system, we demonstrate a specific and gradual decrease of the NDA1 transcript after exposing the plants to 5 degrees C. After 6 days of cold treatment the NDA1 transcript abundance is 10% of the original level. This decrease is accompanied by specific decreases of immunodetected NDA protein and internal rotenone-insensitive... (More)
- Cold stress effects on the expression of genes for respiratory chain enzymes were investigated in potato (Solanum tuberosum L., cv. Desiree) leaves. The nda1 and ndb1 genes, homologues to genes encoding the non-proton-pumping respiratory chain NADH dehydrogenases of Escherichia coli and yeast, were compared to genes encoding catalytic subunits of the proton-pumping NADH dehydrogenase (complex I). Using a real-time PCR system, we demonstrate a specific and gradual decrease of the NDA1 transcript after exposing the plants to 5 degrees C. After 6 days of cold treatment the NDA1 transcript abundance is 10% of the original level. This decrease is accompanied by specific decreases of immunodetected NDA protein and internal rotenone-insensitive NADH oxidation in mitochondria isolated from cold-treated plants. The alternative oxidase is not cold-induced neither at the protein nor at the activity level. The results are discussed in relation to the recent finding that the nda1 gene expression is completely light-dependent. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/108807
- author
- Svensson, Staffan LU ; Johansson, Fredrik I LU ; Møller, Ian M and Rasmusson, Allan LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Plant, Rotenone : pharmacology, Cold, RNA, Messenger : biosynthesis, Mitochondria : enzymology, NADH Dehydrogenase : genetics, NADH Dehydrogenase : metabolism, Oxidation-Reduction : drug effects, Plant Leaves : enzymology, Oxidoreductases : metabolism, Plant Leaves : genetics, Potatoes : enzymology, Potatoes : genetics, Messenger : analysis, Genes, Gene Expression Regulation
- in
- FEBS Letters
- volume
- 517
- issue
- 1-3
- pages
- 79 - 82
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000175225600016
- pmid:12062413
- scopus:0037165621
- ISSN
- 1873-3468
- DOI
- 10.1016/S0014-5793(02)02581-4
- language
- English
- LU publication?
- yes
- id
- b4647b01-6c98-40c1-8364-1c099c1ca56a (old id 108807)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12062413&dopt=Abstract
- date added to LUP
- 2016-04-01 16:42:52
- date last changed
- 2024-01-11 13:20:40
@article{b4647b01-6c98-40c1-8364-1c099c1ca56a, abstract = {{Cold stress effects on the expression of genes for respiratory chain enzymes were investigated in potato (Solanum tuberosum L., cv. Desiree) leaves. The nda1 and ndb1 genes, homologues to genes encoding the non-proton-pumping respiratory chain NADH dehydrogenases of Escherichia coli and yeast, were compared to genes encoding catalytic subunits of the proton-pumping NADH dehydrogenase (complex I). Using a real-time PCR system, we demonstrate a specific and gradual decrease of the NDA1 transcript after exposing the plants to 5 degrees C. After 6 days of cold treatment the NDA1 transcript abundance is 10% of the original level. This decrease is accompanied by specific decreases of immunodetected NDA protein and internal rotenone-insensitive NADH oxidation in mitochondria isolated from cold-treated plants. The alternative oxidase is not cold-induced neither at the protein nor at the activity level. The results are discussed in relation to the recent finding that the nda1 gene expression is completely light-dependent.}}, author = {{Svensson, Staffan and Johansson, Fredrik I and Møller, Ian M and Rasmusson, Allan}}, issn = {{1873-3468}}, keywords = {{Plant; Rotenone : pharmacology; Cold; RNA; Messenger : biosynthesis; Mitochondria : enzymology; NADH Dehydrogenase : genetics; NADH Dehydrogenase : metabolism; Oxidation-Reduction : drug effects; Plant Leaves : enzymology; Oxidoreductases : metabolism; Plant Leaves : genetics; Potatoes : enzymology; Potatoes : genetics; Messenger : analysis; Genes; Gene Expression Regulation}}, language = {{eng}}, number = {{1-3}}, pages = {{79--82}}, publisher = {{Wiley-Blackwell}}, series = {{FEBS Letters}}, title = {{Cold stress decreases the capacity for respiratory NADH oxidation in potato leaves.}}, url = {{http://dx.doi.org/10.1016/S0014-5793(02)02581-4}}, doi = {{10.1016/S0014-5793(02)02581-4}}, volume = {{517}}, year = {{2002}}, }