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Platelet-derived growth factor promotes survival of rat and human mesencephalic dopaminergic neurons in culture

Nikkhah, G ; Odin, Per LU orcid ; Smits, A ; Tingström, Anders LU ; Sanmartin, Agneta LU ; Brundin, Patrik LU ; Funa, K and Lindvall, Olle LU (1993) In Experimental Brain Research 92(3). p.516-523
Abstract
The effect of two isoforms of platelet-derived growth factor (PDGF), PDGF-AA and PDGF-BB, was tested on dissociated cell cultures of ventral mesencephalon from rat and human embryos. PDGF-BB but not PDGF-AA reduced the progressive loss of tyrosine hydroxylase- (TH)-positive neurons in rat and human cell cultures. The mean number of TH-positive cells in the PDGF-BB-treated rat culture was 64% and 106% higher than in the control cultures after 7 and 10 days in vitro, respectively. Corresponding figures for human TH-positive neurons were 90% and 145%. The influence of PDGF-BB was specific for TH-positive neurons and not a general trophic effect, since no change of either total cell number or metabolic activity was found. In PDGF-BB-treated... (More)
The effect of two isoforms of platelet-derived growth factor (PDGF), PDGF-AA and PDGF-BB, was tested on dissociated cell cultures of ventral mesencephalon from rat and human embryos. PDGF-BB but not PDGF-AA reduced the progressive loss of tyrosine hydroxylase- (TH)-positive neurons in rat and human cell cultures. The mean number of TH-positive cells in the PDGF-BB-treated rat culture was 64% and 106% higher than in the control cultures after 7 and 10 days in vitro, respectively. Corresponding figures for human TH-positive neurons were 90% and 145%. The influence of PDGF-BB was specific for TH-positive neurons and not a general trophic effect, since no change of either total cell number or metabolic activity was found. In PDGF-BB-treated cultures of human but not rat tissue the TH-positive neurons had longer neurites than observed in control or PDGF-AA-treated cultures. These data indicate that PDGF-BB may act as a trophic factor for mesencephalic dopaminergic neurons and suggest that administration of PDGF-BB could ameliorate degeneration and possibly promote axonal sprouting of these neurons in vivo. (Less)
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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Dopaminergic neurons, Platelet-derived growth factor, Cell culture, Parkinson's disease, Rat, Human
in
Experimental Brain Research
volume
92
issue
3
pages
516 - 523
publisher
Springer
external identifiers
  • pmid:8095907
  • scopus:0027082893
ISSN
0014-4819
DOI
10.1007/BF00229041
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neurology, Lund (013027000), Molecular Psychiatry Unit (013024100), Neuronal Survival (013212041)
id
b965dfa5-eda2-4ffc-814e-69b1fe285368 (old id 1107589)
date added to LUP
2016-04-01 11:50:54
date last changed
2021-01-03 06:41:58
@article{b965dfa5-eda2-4ffc-814e-69b1fe285368,
  abstract     = {{The effect of two isoforms of platelet-derived growth factor (PDGF), PDGF-AA and PDGF-BB, was tested on dissociated cell cultures of ventral mesencephalon from rat and human embryos. PDGF-BB but not PDGF-AA reduced the progressive loss of tyrosine hydroxylase- (TH)-positive neurons in rat and human cell cultures. The mean number of TH-positive cells in the PDGF-BB-treated rat culture was 64% and 106% higher than in the control cultures after 7 and 10 days in vitro, respectively. Corresponding figures for human TH-positive neurons were 90% and 145%. The influence of PDGF-BB was specific for TH-positive neurons and not a general trophic effect, since no change of either total cell number or metabolic activity was found. In PDGF-BB-treated cultures of human but not rat tissue the TH-positive neurons had longer neurites than observed in control or PDGF-AA-treated cultures. These data indicate that PDGF-BB may act as a trophic factor for mesencephalic dopaminergic neurons and suggest that administration of PDGF-BB could ameliorate degeneration and possibly promote axonal sprouting of these neurons in vivo.}},
  author       = {{Nikkhah, G and Odin, Per and Smits, A and Tingström, Anders and Sanmartin, Agneta and Brundin, Patrik and Funa, K and Lindvall, Olle}},
  issn         = {{0014-4819}},
  keywords     = {{Dopaminergic neurons; Platelet-derived growth factor; Cell culture; Parkinson's disease; Rat; Human}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{516--523}},
  publisher    = {{Springer}},
  series       = {{Experimental Brain Research}},
  title        = {{Platelet-derived growth factor promotes survival of rat and human mesencephalic dopaminergic neurons in culture}},
  url          = {{http://dx.doi.org/10.1007/BF00229041}},
  doi          = {{10.1007/BF00229041}},
  volume       = {{92}},
  year         = {{1993}},
}