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Selective screening of secretory vesicle-associated proteins for autoantigens in type 1 diabetes: VAMP2 and NPY are new minor autoantigens

Hirai, Hiroki ; Miura, Junnosuke ; Hu, Yafang ; Larsson, Helena LU orcid ; Larsson, Karin LU ; Lernmark, Åke LU orcid ; Ivarsson, Sten LU ; Wu, Tianxia ; Kingman, Albert and Tzioufas, Athanasios G , et al. (2008) In Clinical Immunology 127(3). p.366-374
Abstract
The four major autoantigens (IA-2, IA-2 beta, GAD65 and insulin) of type 1 diabetes are all associated with dense core or synaptic vesicles. This raised the possibility that other secretory vesicle-associated proteins might be targets of the autoimmune response in type 1 diabetes. To test this hypothesis 56 proteins, two-thirds of which are associated with secretory vesicles, were prepared by in vitro transcription/translation and screened for autoantibodies by liquid phase radioimmunoprecipitation. Two secretory vesicle-associated proteins, VAMP2 and NPY, were identified as new minor autoantigens with 21% and 9%, respectively, of 200 type 1 diabetes sera reacting positively. These findings add support to the hypothesis that secretory... (More)
The four major autoantigens (IA-2, IA-2 beta, GAD65 and insulin) of type 1 diabetes are all associated with dense core or synaptic vesicles. This raised the possibility that other secretory vesicle-associated proteins might be targets of the autoimmune response in type 1 diabetes. To test this hypothesis 56 proteins, two-thirds of which are associated with secretory vesicles, were prepared by in vitro transcription/translation and screened for autoantibodies by liquid phase radioimmunoprecipitation. Two secretory vesicle-associated proteins, VAMP2 and NPY, were identified as new minor autoantigens with 21% and 9%, respectively, of 200 type 1 diabetes sera reacting positively. These findings add support to the hypothesis that secretory vesicle-associated proteins are particularly important, but not the exclusive, targets of the autoimmune response in type 1 diabetes. Selective screening of the human proteome offers a useful approach for identifying new autoantigens in autoimmune diseases. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
type 1 diabetes, secretory vesicles, proteome, phosphatase, protein tyrosine, IA-2, GAD65, autoantibodies, autoantigens
in
Clinical Immunology
volume
127
issue
3
pages
366 - 374
publisher
Elsevier
external identifiers
  • wos:000256373600014
  • scopus:45249111131
  • pmid:18359275
ISSN
1521-6616
DOI
10.1016/j.clim.2008.01.018
language
English
LU publication?
yes
id
0553cb5f-f2bb-40ca-9421-032dd731a8cc (old id 1201432)
date added to LUP
2016-04-01 11:39:21
date last changed
2024-10-08 04:50:36
@article{0553cb5f-f2bb-40ca-9421-032dd731a8cc,
  abstract     = {{The four major autoantigens (IA-2, IA-2 beta, GAD65 and insulin) of type 1 diabetes are all associated with dense core or synaptic vesicles. This raised the possibility that other secretory vesicle-associated proteins might be targets of the autoimmune response in type 1 diabetes. To test this hypothesis 56 proteins, two-thirds of which are associated with secretory vesicles, were prepared by in vitro transcription/translation and screened for autoantibodies by liquid phase radioimmunoprecipitation. Two secretory vesicle-associated proteins, VAMP2 and NPY, were identified as new minor autoantigens with 21% and 9%, respectively, of 200 type 1 diabetes sera reacting positively. These findings add support to the hypothesis that secretory vesicle-associated proteins are particularly important, but not the exclusive, targets of the autoimmune response in type 1 diabetes. Selective screening of the human proteome offers a useful approach for identifying new autoantigens in autoimmune diseases.}},
  author       = {{Hirai, Hiroki and Miura, Junnosuke and Hu, Yafang and Larsson, Helena and Larsson, Karin and Lernmark, Åke and Ivarsson, Sten and Wu, Tianxia and Kingman, Albert and Tzioufas, Athanasios G and Notkins, Abner L}},
  issn         = {{1521-6616}},
  keywords     = {{type 1 diabetes; secretory vesicles; proteome; phosphatase; protein tyrosine; IA-2; GAD65; autoantibodies; autoantigens}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{366--374}},
  publisher    = {{Elsevier}},
  series       = {{Clinical Immunology}},
  title        = {{Selective screening of secretory vesicle-associated proteins for autoantigens in type 1 diabetes: VAMP2 and NPY are new minor autoantigens}},
  url          = {{http://dx.doi.org/10.1016/j.clim.2008.01.018}},
  doi          = {{10.1016/j.clim.2008.01.018}},
  volume       = {{127}},
  year         = {{2008}},
}