Partitioning of beta-mannanase and alpha-galactosidase from Aspergillus niger in Ucon/Reppal aqueous two-phase systems and using temperature-induced phase separation
(1996) In Bioseparation 6(3). p.147-157- Abstract
- Enzyme partitioning and recovery with a new aqueous two-phase system based on commercially available hydroxypropyl starch Reppal PES 200 and the thermo-separating polymer Ucon 50-HB-5100 was studied. Ucon is an ethylene oxide-propylene oxide random copolymer. A culture supernatant of Aspergillus niger containing extracellular beta-mannanase and alpha-galactosidase was partitioned in two steps. The primary aqueous two-phase system contained Ucon and Reppal as phase forming polymers. The effect on enzyme partitioning of salt composition, salt concentration, pH and polymer concentration was studied with the aim of obtaining optimal partitioning of target enzymes to the phase containing the thermoseparating Ucon polymer. The partitioning of... (More)
- Enzyme partitioning and recovery with a new aqueous two-phase system based on commercially available hydroxypropyl starch Reppal PES 200 and the thermo-separating polymer Ucon 50-HB-5100 was studied. Ucon is an ethylene oxide-propylene oxide random copolymer. A culture supernatant of Aspergillus niger containing extracellular beta-mannanase and alpha-galactosidase was partitioned in two steps. The primary aqueous two-phase system contained Ucon and Reppal as phase forming polymers. The effect on enzyme partitioning of salt composition, salt concentration, pH and polymer concentration was studied with the aim of obtaining optimal partitioning of target enzymes to the phase containing the thermoseparating Ucon polymer. The partitioning of the enzymes could be strongly influenced by addition of the hydrophobic triethyl ammonium ion and the chaotropic perchlorate ion. Also the effect of cationic surfactant, cetyl trimethyl ammonium bromide, on enzyme partitioning was studied. In the second step, temperature induced phase separation was carried out on the isolated Ucon phase. A water phase and a concentrated aqueous Ucon phase were formed. The enzymes were obtained in the water phase almost free of polymer. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/126268
- author
- Farkas, T ; Stålbrand, Henrik LU and Tjerneld, Folke LU
- organization
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- TRICHODERMA-REESEI, HYDROXYPROPYL STARCH, 2-PHASE SYSTEMS, ENZYME-PURIFICATION, POLY(ETHYLENE GLYCOL), LARGE-SCALE, MACROMOLECULES, POLYMERS
- in
- Bioseparation
- volume
- 6
- issue
- 3
- pages
- 147 - 157
- publisher
- Springer
- external identifiers
-
- scopus:0030175223
- ISSN
- 1573-8272
- language
- English
- LU publication?
- yes
- id
- 1525e1ae-7b03-456e-afa4-a599cb5c9fca (old id 126268)
- alternative location
- http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=PubMed&list_uids=8987681&dopt=Abstract
- date added to LUP
- 2016-04-01 15:21:55
- date last changed
- 2022-02-27 06:36:33
@article{1525e1ae-7b03-456e-afa4-a599cb5c9fca, abstract = {{Enzyme partitioning and recovery with a new aqueous two-phase system based on commercially available hydroxypropyl starch Reppal PES 200 and the thermo-separating polymer Ucon 50-HB-5100 was studied. Ucon is an ethylene oxide-propylene oxide random copolymer. A culture supernatant of Aspergillus niger containing extracellular beta-mannanase and alpha-galactosidase was partitioned in two steps. The primary aqueous two-phase system contained Ucon and Reppal as phase forming polymers. The effect on enzyme partitioning of salt composition, salt concentration, pH and polymer concentration was studied with the aim of obtaining optimal partitioning of target enzymes to the phase containing the thermoseparating Ucon polymer. The partitioning of the enzymes could be strongly influenced by addition of the hydrophobic triethyl ammonium ion and the chaotropic perchlorate ion. Also the effect of cationic surfactant, cetyl trimethyl ammonium bromide, on enzyme partitioning was studied. In the second step, temperature induced phase separation was carried out on the isolated Ucon phase. A water phase and a concentrated aqueous Ucon phase were formed. The enzymes were obtained in the water phase almost free of polymer.}}, author = {{Farkas, T and Stålbrand, Henrik and Tjerneld, Folke}}, issn = {{1573-8272}}, keywords = {{TRICHODERMA-REESEI; HYDROXYPROPYL STARCH; 2-PHASE SYSTEMS; ENZYME-PURIFICATION; POLY(ETHYLENE GLYCOL); LARGE-SCALE; MACROMOLECULES; POLYMERS}}, language = {{eng}}, number = {{3}}, pages = {{147--157}}, publisher = {{Springer}}, series = {{Bioseparation}}, title = {{Partitioning of beta-mannanase and alpha-galactosidase from Aspergillus niger in Ucon/Reppal aqueous two-phase systems and using temperature-induced phase separation}}, url = {{http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=PubMed&list_uids=8987681&dopt=Abstract}}, volume = {{6}}, year = {{1996}}, }