Regulation of the eicosanoid pathway by tumour necrosis factor alpha and leukotriene D(4) in intestinal epithelial cells.
(2008) In Prostaglandins, Leukotrienes and Essential Fatty Acids 79(6). p.223-231- Abstract
- In this study the mRNA and protein levels of the key enzymes involved in eicosanoid biosynthesis and the cysteinyl leukotriene receptors (CysLT(1)R and CysLT(2)R) have been analysed in non-transformed intestinal epithelial and colon cancer cell lines. Our results revealed that tumour necrosis factor alpha (TNF-alpha), and leukotriene D(4) (LTD(4)), which are inflammatory mediators implicated in carcinogenesis, stimulated an increase of cyclooxygenase-2 (COX-2), in non-transformed epithelial cells, and 5-lipoxygenase (5-LO) in both non-transformed and cancer cell lines. Furthermore, these mediators also stimulated an up-regulation of LTC(4) synthase in cancer cells as well as non-transformed cells. We also observed an endogenous production... (More)
- In this study the mRNA and protein levels of the key enzymes involved in eicosanoid biosynthesis and the cysteinyl leukotriene receptors (CysLT(1)R and CysLT(2)R) have been analysed in non-transformed intestinal epithelial and colon cancer cell lines. Our results revealed that tumour necrosis factor alpha (TNF-alpha), and leukotriene D(4) (LTD(4)), which are inflammatory mediators implicated in carcinogenesis, stimulated an increase of cyclooxygenase-2 (COX-2), in non-transformed epithelial cells, and 5-lipoxygenase (5-LO) in both non-transformed and cancer cell lines. Furthermore, these mediators also stimulated an up-regulation of LTC(4) synthase in cancer cells as well as non-transformed cells. We also observed an endogenous production of CysLTs in these cells. TNF-alpha and LTD(4), to a lesser extent, up-regulate the CysLT(1)R levels. Interestingly, TNF-alpha also reduced CysLT(2)R expression in cancer cells. Our results demonstrate that inflammatory mediators can cause intestinal epithelial cells to up-regulate the expression of enzymes needed for the biosynthesis of eicosanoids, including the cysteinyl leukotrienes, as well as the signal transducing proteins, the CysLT receptors, thus providing important mechanisms for both maintaining inflammation and for tumour progression. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1276653
- author
- Yudina, Yulyana LU ; Parhamifar, Ladan LU ; Bengtsson, Astrid LU ; Juhas, Maria LU and Sjölander, Anita LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Prostaglandins, Leukotrienes and Essential Fatty Acids
- volume
- 79
- issue
- 6
- pages
- 223 - 231
- publisher
- Elsevier
- external identifiers
-
- wos:000261994800006
- pmid:19042113
- scopus:61549132288
- pmid:19042113
- ISSN
- 0952-3278
- DOI
- 10.1016/j.plefa.2008.09.024
- language
- English
- LU publication?
- yes
- id
- 1a887eae-d94a-4591-afd8-1e3c1e1a03d8 (old id 1276653)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/19042113?dopt=Abstract
- date added to LUP
- 2016-04-04 07:53:56
- date last changed
- 2022-03-07 20:51:47
@article{1a887eae-d94a-4591-afd8-1e3c1e1a03d8,
abstract = {{In this study the mRNA and protein levels of the key enzymes involved in eicosanoid biosynthesis and the cysteinyl leukotriene receptors (CysLT(1)R and CysLT(2)R) have been analysed in non-transformed intestinal epithelial and colon cancer cell lines. Our results revealed that tumour necrosis factor alpha (TNF-alpha), and leukotriene D(4) (LTD(4)), which are inflammatory mediators implicated in carcinogenesis, stimulated an increase of cyclooxygenase-2 (COX-2), in non-transformed epithelial cells, and 5-lipoxygenase (5-LO) in both non-transformed and cancer cell lines. Furthermore, these mediators also stimulated an up-regulation of LTC(4) synthase in cancer cells as well as non-transformed cells. We also observed an endogenous production of CysLTs in these cells. TNF-alpha and LTD(4), to a lesser extent, up-regulate the CysLT(1)R levels. Interestingly, TNF-alpha also reduced CysLT(2)R expression in cancer cells. Our results demonstrate that inflammatory mediators can cause intestinal epithelial cells to up-regulate the expression of enzymes needed for the biosynthesis of eicosanoids, including the cysteinyl leukotrienes, as well as the signal transducing proteins, the CysLT receptors, thus providing important mechanisms for both maintaining inflammation and for tumour progression.}},
author = {{Yudina, Yulyana and Parhamifar, Ladan and Bengtsson, Astrid and Juhas, Maria and Sjölander, Anita}},
issn = {{0952-3278}},
language = {{eng}},
number = {{6}},
pages = {{223--231}},
publisher = {{Elsevier}},
series = {{Prostaglandins, Leukotrienes and Essential Fatty Acids}},
title = {{Regulation of the eicosanoid pathway by tumour necrosis factor alpha and leukotriene D(4) in intestinal epithelial cells.}},
url = {{http://dx.doi.org/10.1016/j.plefa.2008.09.024}},
doi = {{10.1016/j.plefa.2008.09.024}},
volume = {{79}},
year = {{2008}},
}