Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

A capillary-based amperometric flow immunoassay for 2,4,6-trichlorophenol

Nistor, Catalin LU and Emnéus, Jenny LU (2003) In Analytical and Bioanalytical Chemistry 375(1). p.125-132
Abstract
This paper describes the development of two different capillary-based heterogeneous competitive flow immunoassay formats (capillary flow injection immunoassay (CFIIA) and capillary sequential injection immunoassay (CSIIA)) for the determination of 2,4,6-trichlorophenol (2,4,6-TCP). The assays are based on the competition between the analyte and an analyte derivative labelled with the enzyme #-galactosidase, for an anti-TCP antibody, followed by the injection of the mixture at equilibrium into a flow stream, where separation between the fractions bound and unbound to the antibody is performed in a glass capillary containing immobilised protein A. The antibody-tracer fraction retained inside the protein A capillary was measured by injection... (More)
This paper describes the development of two different capillary-based heterogeneous competitive flow immunoassay formats (capillary flow injection immunoassay (CFIIA) and capillary sequential injection immunoassay (CSIIA)) for the determination of 2,4,6-trichlorophenol (2,4,6-TCP). The assays are based on the competition between the analyte and an analyte derivative labelled with the enzyme #-galactosidase, for an anti-TCP antibody, followed by the injection of the mixture at equilibrium into a flow stream, where separation between the fractions bound and unbound to the antibody is performed in a glass capillary containing immobilised protein A. The antibody-tracer fraction retained inside the protein A capillary was measured by injection of 4-aminophenyl-#-D-galactoside (4-APG), followed by amperometric detection of the enzymatically generated 4-aminophenol (4-AP), leading to a negative correlation between the signal and the analyte concentration. (Less)
Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical and Bioanalytical Chemistry
volume
375
issue
1
pages
125 - 132
publisher
Springer
external identifiers
  • pmid:12520448
  • wos:000180848800030
  • scopus:0037268719
ISSN
1618-2642
DOI
10.1007/s00216-002-1631-1
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
id
89f9bdd4-2275-4fb1-aac5-77f3e3fc9708 (old id 128960)
date added to LUP
2016-04-01 11:50:58
date last changed
2024-07-02 13:20:56
@article{89f9bdd4-2275-4fb1-aac5-77f3e3fc9708,
  abstract     = {{This paper describes the development of two different capillary-based heterogeneous competitive flow immunoassay formats (capillary flow injection immunoassay (CFIIA) and capillary sequential injection immunoassay (CSIIA)) for the determination of 2,4,6-trichlorophenol (2,4,6-TCP). The assays are based on the competition between the analyte and an analyte derivative labelled with the enzyme #-galactosidase, for an anti-TCP antibody, followed by the injection of the mixture at equilibrium into a flow stream, where separation between the fractions bound and unbound to the antibody is performed in a glass capillary containing immobilised protein A. The antibody-tracer fraction retained inside the protein A capillary was measured by injection of 4-aminophenyl-#-D-galactoside (4-APG), followed by amperometric detection of the enzymatically generated 4-aminophenol (4-AP), leading to a negative correlation between the signal and the analyte concentration.}},
  author       = {{Nistor, Catalin and Emnéus, Jenny}},
  issn         = {{1618-2642}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{125--132}},
  publisher    = {{Springer}},
  series       = {{Analytical and Bioanalytical Chemistry}},
  title        = {{A capillary-based amperometric flow immunoassay for 2,4,6-trichlorophenol}},
  url          = {{http://dx.doi.org/10.1007/s00216-002-1631-1}},
  doi          = {{10.1007/s00216-002-1631-1}},
  volume       = {{375}},
  year         = {{2003}},
}