Similitude of permeabilities for Ficoll, pullulan, charge-modified albumin and native albumin across the rat peritoneal membrane.
(2009) In Acta Physiologica 196(4). p.427-433- Abstract
- Abstract Aim: Compared to neutral globular proteins, neutral polysaccharides, such as dextran, pullulan and Ficoll, appear hyperpermeable across the glomerular filtration barrier. This has been attributed to an increased flexibility and/or asymmetry of polysaccharides. The present study investigates whether polysaccharides are hyperpermeable also across the continuous capillaries in the rat peritoneum. Methods: In anesthetized Wistar rats, FITC-Ficoll or FITC-pullulan together with (125)I-human serum albumin (RISA) or neutralized (125)I-bovine serum albumin (nBSA) were given intravenously, after which peritoneal dialysis using conventional peritoneal dialysis fluid (Gambrosol 1.5%) was performed for 120 min. Concentrations of... (More)
- Abstract Aim: Compared to neutral globular proteins, neutral polysaccharides, such as dextran, pullulan and Ficoll, appear hyperpermeable across the glomerular filtration barrier. This has been attributed to an increased flexibility and/or asymmetry of polysaccharides. The present study investigates whether polysaccharides are hyperpermeable also across the continuous capillaries in the rat peritoneum. Methods: In anesthetized Wistar rats, FITC-Ficoll or FITC-pullulan together with (125)I-human serum albumin (RISA) or neutralized (125)I-bovine serum albumin (nBSA) were given intravenously, after which peritoneal dialysis using conventional peritoneal dialysis fluid (Gambrosol 1.5%) was performed for 120 min. Concentrations of FITC-polysaccharides and radioactive albumin species in plasma and dialysis fluid were analyzed with high performance size exclusion chromatography and a gamma counter, respectively. Transperitoneal clearance values were calculated for polysaccharides in the molecular radius range 36-150 A, and for RISA and nBSA. Results: Ficoll and pullulan showed more or less identical permeabilities, compared to RISA and nBSA, across the peritoneal membrane. Although RISA-clearance, 5.50+/-0.28 (muL/min; +/-SEM), tended to be lower than the clearances of Ficoll(36A) (6.55+/-0.25), pullulan(36A) (6.08+/-0.22) and nBSA (6.56+/-0.23), the difference was not statistically significant. This is in contrast to the hyperpermeability exhibited by polysaccharides across the glomerular filtration barrier and also contrasts with the charge selectivity of the latter. Conclusion: The phenomenon of molecular flexibility is more important for a macromolecule's permeability through the glomerular filter than across the continuous peritoneal capillary endothelium. Furthermore, it seems that charge plays a subordinate role in the steady-state transport across the combined peritoneal capillary-interstitial barrier. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1289744
- author
- Asgeirsson, Daniel LU ; Axelsson, Josefin LU ; Rippe, Catarina LU and Rippe, Bengt LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Acta Physiologica
- volume
- 196
- issue
- 4
- pages
- 427 - 433
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000267948300008
- pmid:19141139
- scopus:67650434858
- ISSN
- 1748-1708
- DOI
- 10.1111/j.1748-1716.2009.01955.x
- language
- English
- LU publication?
- yes
- id
- fa81b6ea-e61d-45b3-bb65-a829a69a767e (old id 1289744)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/19141139?dopt=Abstract
- date added to LUP
- 2016-04-01 11:49:36
- date last changed
- 2022-01-26 18:46:57
@article{fa81b6ea-e61d-45b3-bb65-a829a69a767e,
abstract = {{Abstract Aim: Compared to neutral globular proteins, neutral polysaccharides, such as dextran, pullulan and Ficoll, appear hyperpermeable across the glomerular filtration barrier. This has been attributed to an increased flexibility and/or asymmetry of polysaccharides. The present study investigates whether polysaccharides are hyperpermeable also across the continuous capillaries in the rat peritoneum. Methods: In anesthetized Wistar rats, FITC-Ficoll or FITC-pullulan together with (125)I-human serum albumin (RISA) or neutralized (125)I-bovine serum albumin (nBSA) were given intravenously, after which peritoneal dialysis using conventional peritoneal dialysis fluid (Gambrosol 1.5%) was performed for 120 min. Concentrations of FITC-polysaccharides and radioactive albumin species in plasma and dialysis fluid were analyzed with high performance size exclusion chromatography and a gamma counter, respectively. Transperitoneal clearance values were calculated for polysaccharides in the molecular radius range 36-150 A, and for RISA and nBSA. Results: Ficoll and pullulan showed more or less identical permeabilities, compared to RISA and nBSA, across the peritoneal membrane. Although RISA-clearance, 5.50+/-0.28 (muL/min; +/-SEM), tended to be lower than the clearances of Ficoll(36A) (6.55+/-0.25), pullulan(36A) (6.08+/-0.22) and nBSA (6.56+/-0.23), the difference was not statistically significant. This is in contrast to the hyperpermeability exhibited by polysaccharides across the glomerular filtration barrier and also contrasts with the charge selectivity of the latter. Conclusion: The phenomenon of molecular flexibility is more important for a macromolecule's permeability through the glomerular filter than across the continuous peritoneal capillary endothelium. Furthermore, it seems that charge plays a subordinate role in the steady-state transport across the combined peritoneal capillary-interstitial barrier.}},
author = {{Asgeirsson, Daniel and Axelsson, Josefin and Rippe, Catarina and Rippe, Bengt}},
issn = {{1748-1708}},
language = {{eng}},
number = {{4}},
pages = {{427--433}},
publisher = {{Wiley-Blackwell}},
series = {{Acta Physiologica}},
title = {{Similitude of permeabilities for Ficoll, pullulan, charge-modified albumin and native albumin across the rat peritoneal membrane.}},
url = {{http://dx.doi.org/10.1111/j.1748-1716.2009.01955.x}},
doi = {{10.1111/j.1748-1716.2009.01955.x}},
volume = {{196}},
year = {{2009}},
}