Gene expression profiling of arthritis using a QTL chip reveals a complex gene regulation of the Cia5 region in mice.
(2005) In Genes and Immunity 6(7). p.575-583- Abstract
- One of the major quantitative trait loci (QTLs) associated with arthritis in crosses between B10.RIII and RIIIS/J mice is the Cia5 on chromosome 3. Early in the congenic mapping process it was clear that the locus was complex, consisting of several subloci with small effects. Therefore, we developed two novel strategies to dissect a QTL: the partial advanced intercross (PAI) strategy, with which we recently found the Cia5 region to consist of three loci, Cia5, Cia21 and Cia22, and now we introduce the QTL-chip strategy, where we have combined congenic mapping with a QTL-restricted expression profiling using a novel microarray design. The expression of QTL genes was compared between parental and congenic mice in lymph node, spleen and paw... (More)
- One of the major quantitative trait loci (QTLs) associated with arthritis in crosses between B10.RIII and RIIIS/J mice is the Cia5 on chromosome 3. Early in the congenic mapping process it was clear that the locus was complex, consisting of several subloci with small effects. Therefore, we developed two novel strategies to dissect a QTL: the partial advanced intercross (PAI) strategy, with which we recently found the Cia5 region to consist of three loci, Cia5, Cia21 and Cia22, and now we introduce the QTL-chip strategy, where we have combined congenic mapping with a QTL-restricted expression profiling using a novel microarray design. The expression of QTL genes was compared between parental and congenic mice in lymph node, spleen and paw samples in five biological replicates and in dye-swapped experiments at three time points during the induction phase of arthritis. The QTL chip approach revealed 4 genes located in Cia21, differently expressed in lymph nodes, and 14 genes in Cia22, located within two clusters. One cluster contains six genes, differently expressed in spleen, and the second cluster contains eight genes, differently expressed in paws. We conclude the QTL-chip strategy to be valuable in the selection of candidate genes to be prioritized for further investigation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/142147
- author
- Johannesson, Martina LU ; Olsson, Lina LU ; Lindqvist, Anna-Karin LU ; Moller, S ; Koczan, D ; Wester Rosenlöf, Lena LU ; Thiesen, H-J ; Ibrahim, S and Holmdahl, Rikard LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- microarray, quantitative trait locus, complex trait mapping, collagen-induced arthritis
- in
- Genes and Immunity
- volume
- 6
- issue
- 7
- pages
- 575 - 583
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:16015370
- wos:000232881800003
- scopus:27844524976
- pmid:16015370
- ISSN
- 1476-5470
- DOI
- 10.1038/sj.gene.6364242
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Medical Inflammation Research (013212019)
- id
- d9bc755b-87c6-474a-a95d-d700b42a53c7 (old id 142147)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16015370&dopt=Abstract
- date added to LUP
- 2016-04-01 11:36:04
- date last changed
- 2022-01-26 07:26:20
@article{d9bc755b-87c6-474a-a95d-d700b42a53c7, abstract = {{One of the major quantitative trait loci (QTLs) associated with arthritis in crosses between B10.RIII and RIIIS/J mice is the Cia5 on chromosome 3. Early in the congenic mapping process it was clear that the locus was complex, consisting of several subloci with small effects. Therefore, we developed two novel strategies to dissect a QTL: the partial advanced intercross (PAI) strategy, with which we recently found the Cia5 region to consist of three loci, Cia5, Cia21 and Cia22, and now we introduce the QTL-chip strategy, where we have combined congenic mapping with a QTL-restricted expression profiling using a novel microarray design. The expression of QTL genes was compared between parental and congenic mice in lymph node, spleen and paw samples in five biological replicates and in dye-swapped experiments at three time points during the induction phase of arthritis. The QTL chip approach revealed 4 genes located in Cia21, differently expressed in lymph nodes, and 14 genes in Cia22, located within two clusters. One cluster contains six genes, differently expressed in spleen, and the second cluster contains eight genes, differently expressed in paws. We conclude the QTL-chip strategy to be valuable in the selection of candidate genes to be prioritized for further investigation.}}, author = {{Johannesson, Martina and Olsson, Lina and Lindqvist, Anna-Karin and Moller, S and Koczan, D and Wester Rosenlöf, Lena and Thiesen, H-J and Ibrahim, S and Holmdahl, Rikard}}, issn = {{1476-5470}}, keywords = {{microarray; quantitative trait locus; complex trait mapping; collagen-induced arthritis}}, language = {{eng}}, number = {{7}}, pages = {{575--583}}, publisher = {{Nature Publishing Group}}, series = {{Genes and Immunity}}, title = {{Gene expression profiling of arthritis using a QTL chip reveals a complex gene regulation of the Cia5 region in mice.}}, url = {{http://dx.doi.org/10.1038/sj.gene.6364242}}, doi = {{10.1038/sj.gene.6364242}}, volume = {{6}}, year = {{2005}}, }