A modified ELISA for improved detection of IgA, IgG, and IgM anti-tissue transglutaminase antibodies in celiac disease
(2009) In Clinica Chimica Acta 403(1-2). p.37-41- Abstract
- Background: Serum IgA antibodies against tissue transglutaminase (tTG) are reliable markers for celiac disease (CD), still the diagnostic performance of anti-tTG immunoassays can be improved. A novel ELISA, using fibronectin (FN) as tTG binding protein, was evaluated for the detection of anti-tTG antibodies in CD. Methods: Sera from 173 children with untreated CD and 97 controls were analyzed for IgA, IgG, and IgM anti-tTG antibodies with ELISA using human recombinant tTG with or without FN. Results: The areas under the ROC (receiver operating characteristic) curves were significantly higher for FN/tTG ELISA compared to tTG ELISA for IgG (0.930 versus 0.809; p < 0.001) and IgM (0.850 versus 0.811; p = 0.019), but not for IgA (0.977... (More)
- Background: Serum IgA antibodies against tissue transglutaminase (tTG) are reliable markers for celiac disease (CD), still the diagnostic performance of anti-tTG immunoassays can be improved. A novel ELISA, using fibronectin (FN) as tTG binding protein, was evaluated for the detection of anti-tTG antibodies in CD. Methods: Sera from 173 children with untreated CD and 97 controls were analyzed for IgA, IgG, and IgM anti-tTG antibodies with ELISA using human recombinant tTG with or without FN. Results: The areas under the ROC (receiver operating characteristic) curves were significantly higher for FN/tTG ELISA compared to tTG ELISA for IgG (0.930 versus 0.809; p < 0.001) and IgM (0.850 versus 0.811; p = 0.019), but not for IgA (0.977 versus 0.970; p = 0.356), respectively. At the fixed diagnostic specificity (100% for IgA and IgM, 99% for IgG), the sensitivity of all three FN/tTG ELISA (92.5% for IgA, 60.7% for IgG, 50.3% for IgM) exceeded those obtained in tTG ELISA (89.0% for IgA, 48.6% for IgG, 38.7% for IgM; p < 0.05). The combined use of IgA- and IgG-FN/tTG ELISA resulted in 95.4% sensitivity and 99.0% specificity for CD. Conclusions: Using FN to bind tTG improves the diagnostic performance of solid phase anti-tTG antibody assays for childhood CD. (C) 2009 Elsevier B.V. All rights reserved. (Less)
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https://lup.lub.lu.se/record/1425699
- author
- Teesalu, Kaupo ; Agardh, Daniel LU ; Panarina, Marina ; Utt, Meeme LU ; Uibo, Oivi and Uibo, Raivo LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Fibronectin, Anti-tissue transglutaminase antibodies, ELISA, disease, Celiac
- in
- Clinica Chimica Acta
- volume
- 403
- issue
- 1-2
- pages
- 37 - 41
- publisher
- Elsevier
- external identifiers
-
- wos:000266190100006
- scopus:64849087024
- ISSN
- 0009-8981
- DOI
- 10.1016/j.cca.2009.01.006
- language
- English
- LU publication?
- yes
- id
- 1929a7d2-3c77-4646-b8e1-6edbbcd35afe (old id 1425699)
- date added to LUP
- 2016-04-01 11:39:28
- date last changed
- 2022-04-28 18:06:08
@article{1929a7d2-3c77-4646-b8e1-6edbbcd35afe, abstract = {{Background: Serum IgA antibodies against tissue transglutaminase (tTG) are reliable markers for celiac disease (CD), still the diagnostic performance of anti-tTG immunoassays can be improved. A novel ELISA, using fibronectin (FN) as tTG binding protein, was evaluated for the detection of anti-tTG antibodies in CD. Methods: Sera from 173 children with untreated CD and 97 controls were analyzed for IgA, IgG, and IgM anti-tTG antibodies with ELISA using human recombinant tTG with or without FN. Results: The areas under the ROC (receiver operating characteristic) curves were significantly higher for FN/tTG ELISA compared to tTG ELISA for IgG (0.930 versus 0.809; p < 0.001) and IgM (0.850 versus 0.811; p = 0.019), but not for IgA (0.977 versus 0.970; p = 0.356), respectively. At the fixed diagnostic specificity (100% for IgA and IgM, 99% for IgG), the sensitivity of all three FN/tTG ELISA (92.5% for IgA, 60.7% for IgG, 50.3% for IgM) exceeded those obtained in tTG ELISA (89.0% for IgA, 48.6% for IgG, 38.7% for IgM; p < 0.05). The combined use of IgA- and IgG-FN/tTG ELISA resulted in 95.4% sensitivity and 99.0% specificity for CD. Conclusions: Using FN to bind tTG improves the diagnostic performance of solid phase anti-tTG antibody assays for childhood CD. (C) 2009 Elsevier B.V. All rights reserved.}}, author = {{Teesalu, Kaupo and Agardh, Daniel and Panarina, Marina and Utt, Meeme and Uibo, Oivi and Uibo, Raivo}}, issn = {{0009-8981}}, keywords = {{Fibronectin; Anti-tissue transglutaminase antibodies; ELISA; disease; Celiac}}, language = {{eng}}, number = {{1-2}}, pages = {{37--41}}, publisher = {{Elsevier}}, series = {{Clinica Chimica Acta}}, title = {{A modified ELISA for improved detection of IgA, IgG, and IgM anti-tissue transglutaminase antibodies in celiac disease}}, url = {{http://dx.doi.org/10.1016/j.cca.2009.01.006}}, doi = {{10.1016/j.cca.2009.01.006}}, volume = {{403}}, year = {{2009}}, }