Two-dimensional gel electrophoresis of proteins and peptides in bovine milk
(2005) In International Dairy Journal 15(2). p.111-121- Abstract
- Proteins and peptides in bovine milk and colostrum were separated by two-dimensional gel electrophoresis (2-DE). The proteins were separated in the first dimension by isoelectric focusing in the pH ranges 4-7 and 6-11, and in the second dimension by sodium dodecyl sulphate polyacrylamide gel electrophoresis on 12.5% T homogeneous gels. The peptides were separated in the pH range 3-10 in the first dimension, while 15% T homogeneous gels were used in the second dimension. 2-DE evaluation of samples with various somatic cell counts showed an increased number of peptides with increased cell count. 2-DE analysis of samples heated at 65 and 85 degreesC for 30 min showed a decrease in the number of proteins and peptides with increased... (More)
- Proteins and peptides in bovine milk and colostrum were separated by two-dimensional gel electrophoresis (2-DE). The proteins were separated in the first dimension by isoelectric focusing in the pH ranges 4-7 and 6-11, and in the second dimension by sodium dodecyl sulphate polyacrylamide gel electrophoresis on 12.5% T homogeneous gels. The peptides were separated in the pH range 3-10 in the first dimension, while 15% T homogeneous gels were used in the second dimension. 2-DE evaluation of samples with various somatic cell counts showed an increased number of peptides with increased cell count. 2-DE analysis of samples heated at 65 and 85 degreesC for 30 min showed a decrease in the number of proteins and peptides with increased temperature. Colostrum exhibited a peptide pattern in which the intensity and number of spots decreased with time postpartum. These results show that 2-DE is a useful tool to detect variations of proteins and peptides in milk. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/152079
- author
- Lindmark Månsson, Helena LU ; Timgren, Anna LU ; Alden, G and Paulsson, Marie LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Peptides, Proteins, Somatic cell counts, Two-dimensional gel electrophoresis, Colostrum, Bovine milk, Heat treatment
- in
- International Dairy Journal
- volume
- 15
- issue
- 2
- pages
- 111 - 121
- publisher
- Elsevier
- external identifiers
-
- wos:000226570900002
- scopus:11844284815
- ISSN
- 0958-6946
- DOI
- 10.1016/j.idairyj.2004.06.010
- language
- English
- LU publication?
- yes
- id
- a1261cdd-a892-40a8-82db-bf4e719fee4a (old id 152079)
- date added to LUP
- 2016-04-01 16:41:45
- date last changed
- 2023-11-14 15:50:46
@article{a1261cdd-a892-40a8-82db-bf4e719fee4a, abstract = {{Proteins and peptides in bovine milk and colostrum were separated by two-dimensional gel electrophoresis (2-DE). The proteins were separated in the first dimension by isoelectric focusing in the pH ranges 4-7 and 6-11, and in the second dimension by sodium dodecyl sulphate polyacrylamide gel electrophoresis on 12.5% T homogeneous gels. The peptides were separated in the pH range 3-10 in the first dimension, while 15% T homogeneous gels were used in the second dimension. 2-DE evaluation of samples with various somatic cell counts showed an increased number of peptides with increased cell count. 2-DE analysis of samples heated at 65 and 85 degreesC for 30 min showed a decrease in the number of proteins and peptides with increased temperature. Colostrum exhibited a peptide pattern in which the intensity and number of spots decreased with time postpartum. These results show that 2-DE is a useful tool to detect variations of proteins and peptides in milk.}}, author = {{Lindmark Månsson, Helena and Timgren, Anna and Alden, G and Paulsson, Marie}}, issn = {{0958-6946}}, keywords = {{Peptides; Proteins; Somatic cell counts; Two-dimensional gel electrophoresis; Colostrum; Bovine milk; Heat treatment}}, language = {{eng}}, number = {{2}}, pages = {{111--121}}, publisher = {{Elsevier}}, series = {{International Dairy Journal}}, title = {{Two-dimensional gel electrophoresis of proteins and peptides in bovine milk}}, url = {{http://dx.doi.org/10.1016/j.idairyj.2004.06.010}}, doi = {{10.1016/j.idairyj.2004.06.010}}, volume = {{15}}, year = {{2005}}, }