Purification of IgD from human serum - A novel application of recombinant M. catarrhalis IgD-binding protein (MID).
(2006) In Journal of Immunological Methods 317. p.31-37- Abstract
- Moraxella catarrhalis IgD-binding protein (MID) is a multimeric outer membrane protein belonging to the family of autotransporters. The IgD-binding domain of MID is located between amino acids MID 962-1200 and binds to amino acids 198-224 of the IgD C(H)1 region. In the present study, we describe a method to purify IgD from serum with high levels of IgD using a two-step affinity chromatography process. The first step involves depletion of MID-specific antibodies of all classes from serum using the non-IgD-binding fragment MID1000-1200. This step is followed by selective capture of IgD with MID962-1200. Furthermore, we demonstrate that the eluted IgD is pure, intact and functional for use in downstream applications. Our approach reduces the... (More)
- Moraxella catarrhalis IgD-binding protein (MID) is a multimeric outer membrane protein belonging to the family of autotransporters. The IgD-binding domain of MID is located between amino acids MID 962-1200 and binds to amino acids 198-224 of the IgD C(H)1 region. In the present study, we describe a method to purify IgD from serum with high levels of IgD using a two-step affinity chromatography process. The first step involves depletion of MID-specific antibodies of all classes from serum using the non-IgD-binding fragment MID1000-1200. This step is followed by selective capture of IgD with MID962-1200. Furthermore, we demonstrate that the eluted IgD is pure, intact and functional for use in downstream applications. Our approach reduces the non-specificity commonly associated with lectin-based IgD purification regimes that rely on glycosylation of the IgD molecule. (c) 2006 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/162214
- author
- Samuelsson, Martin LU ; Forsgren, Arne LU and Riesbeck, Kristian LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- purification, immunoglobulin D, Moraxella catarrhalis, MID
- in
- Journal of Immunological Methods
- volume
- 317
- pages
- 31 - 37
- publisher
- Elsevier
- external identifiers
-
- wos:000243148700004
- scopus:33751345343
- pmid:17056056
- ISSN
- 1872-7905
- DOI
- 10.1016/j.jim.2006.09.007
- language
- English
- LU publication?
- yes
- id
- 00f587aa-fe85-4f59-8a12-ce406bae0750 (old id 162214)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17056056&dopt=Abstract
- date added to LUP
- 2016-04-01 16:17:08
- date last changed
- 2022-01-28 18:39:23
@article{00f587aa-fe85-4f59-8a12-ce406bae0750, abstract = {{Moraxella catarrhalis IgD-binding protein (MID) is a multimeric outer membrane protein belonging to the family of autotransporters. The IgD-binding domain of MID is located between amino acids MID 962-1200 and binds to amino acids 198-224 of the IgD C(H)1 region. In the present study, we describe a method to purify IgD from serum with high levels of IgD using a two-step affinity chromatography process. The first step involves depletion of MID-specific antibodies of all classes from serum using the non-IgD-binding fragment MID1000-1200. This step is followed by selective capture of IgD with MID962-1200. Furthermore, we demonstrate that the eluted IgD is pure, intact and functional for use in downstream applications. Our approach reduces the non-specificity commonly associated with lectin-based IgD purification regimes that rely on glycosylation of the IgD molecule. (c) 2006 Elsevier B.V. All rights reserved.}}, author = {{Samuelsson, Martin and Forsgren, Arne and Riesbeck, Kristian}}, issn = {{1872-7905}}, keywords = {{purification; immunoglobulin D; Moraxella catarrhalis; MID}}, language = {{eng}}, pages = {{31--37}}, publisher = {{Elsevier}}, series = {{Journal of Immunological Methods}}, title = {{Purification of IgD from human serum - A novel application of recombinant M. catarrhalis IgD-binding protein (MID).}}, url = {{http://dx.doi.org/10.1016/j.jim.2006.09.007}}, doi = {{10.1016/j.jim.2006.09.007}}, volume = {{317}}, year = {{2006}}, }