Increased beta-cell volume in mice fed a high-fat diet A dynamic study over 12 months
(2010) In Islets 2(6). p.353-356- Abstract
- As we previously demonstrated, there is an adaptive increase in insulin secretion in insulin resistance in the model of high-fat fed female mice. Since it is assumed that islets also adapt to insulin resistance with beta-cell expansion, we have now examined beta-cell volume in this experimental model. Female C57BL/6JBomTac mice were therefore fed a high-fat diet (60% fat from lard) for three, six or twelve months and beta-cell volume was estimated as beta-cell area per islet, individual beta-cell size and beta-cell number per islet. Control animals were fed a normal chow (11% fat). We found that beta-cell area per islet and total number of beta-cells per islet were increased already after three months of high-fat feeding and that this... (More)
- As we previously demonstrated, there is an adaptive increase in insulin secretion in insulin resistance in the model of high-fat fed female mice. Since it is assumed that islets also adapt to insulin resistance with beta-cell expansion, we have now examined beta-cell volume in this experimental model. Female C57BL/6JBomTac mice were therefore fed a high-fat diet (60% fat from lard) for three, six or twelve months and beta-cell volume was estimated as beta-cell area per islet, individual beta-cell size and beta-cell number per islet. Control animals were fed a normal chow (11% fat). We found that beta-cell area per islet and total number of beta-cells per islet were increased already after three months of high-fat feeding and that this increase was sustained throughout the twelve month study period. In contrast, individual beta-cell size showed a dynamic pattern with a reduction after three months followed by increase after six and twelve months. The number of apoptosis (caspase-3) positive beta-cells was reduced after three months, whereas there was no difference in proliferation (Ki-67) positive cells, although these were generally rarely observed. Thus, we conclude that insulin resistance accompanying high-fat feeding in mice is followed by progressive beta-cell expansion as evident by early increased islet beta-cell volume and total number of beta-cells, whereas individual beta-cell size showed a dynamic response. The model is also associated with an early reduced apoptosis, which may contribute to the increased beta-cell volume. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1963975
- author
- Ahren, Jonatan ; Ahrén, Bo LU and Wierup, Nils LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- beta cell mass, insulin resistance, islet adaptation, apoptosis, proliferation
- in
- Islets
- volume
- 2
- issue
- 6
- pages
- 353 - 356
- publisher
- Landes Bioscience
- external identifiers
-
- wos:000289689500003
- scopus:78751563884
- ISSN
- 1938-2022
- DOI
- 10.4161/isl.2.6.13619
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neuroendocrine Cell Biology (013212008), Medicine (Lund) (013230025)
- id
- 24060c8a-6945-48a1-976d-8ea0e4e67362 (old id 1963975)
- date added to LUP
- 2016-04-01 10:40:00
- date last changed
- 2024-02-21 21:49:55
@article{24060c8a-6945-48a1-976d-8ea0e4e67362,
abstract = {{As we previously demonstrated, there is an adaptive increase in insulin secretion in insulin resistance in the model of high-fat fed female mice. Since it is assumed that islets also adapt to insulin resistance with beta-cell expansion, we have now examined beta-cell volume in this experimental model. Female C57BL/6JBomTac mice were therefore fed a high-fat diet (60% fat from lard) for three, six or twelve months and beta-cell volume was estimated as beta-cell area per islet, individual beta-cell size and beta-cell number per islet. Control animals were fed a normal chow (11% fat). We found that beta-cell area per islet and total number of beta-cells per islet were increased already after three months of high-fat feeding and that this increase was sustained throughout the twelve month study period. In contrast, individual beta-cell size showed a dynamic pattern with a reduction after three months followed by increase after six and twelve months. The number of apoptosis (caspase-3) positive beta-cells was reduced after three months, whereas there was no difference in proliferation (Ki-67) positive cells, although these were generally rarely observed. Thus, we conclude that insulin resistance accompanying high-fat feeding in mice is followed by progressive beta-cell expansion as evident by early increased islet beta-cell volume and total number of beta-cells, whereas individual beta-cell size showed a dynamic response. The model is also associated with an early reduced apoptosis, which may contribute to the increased beta-cell volume.}},
author = {{Ahren, Jonatan and Ahrén, Bo and Wierup, Nils}},
issn = {{1938-2022}},
keywords = {{beta cell mass; insulin resistance; islet adaptation; apoptosis; proliferation}},
language = {{eng}},
number = {{6}},
pages = {{353--356}},
publisher = {{Landes Bioscience}},
series = {{Islets}},
title = {{Increased beta-cell volume in mice fed a high-fat diet A dynamic study over 12 months}},
url = {{http://dx.doi.org/10.4161/isl.2.6.13619}},
doi = {{10.4161/isl.2.6.13619}},
volume = {{2}},
year = {{2010}},
}