Inhibition of EBF function by active Notch signaling reveals a novel regulatory pathway in early B-cell development
(2005) In Blood 106(6). p.1995-2001- Abstract
- The Notch signaling pathway is involved in several lineage commitment and differentiation events. One of these is fate determination of the common lymphoid progenitor, promoting T-cell development at the expense of B-cell differentiation. It has been suggested that this process relies on Notch's ability to inhibit E proteins, which are crucial for early B-cell development. Here, we report that Notch signaling also modulates the function of the transcription factor, early B-cell factor (EBF). Transient transfection of intracellular Notch1 (Notch1-IC) into a pre-B cell line resulted in the down-regulation of EBF-regulated promoters and diminished the capacity of EBF to activate these promoters in an epithelial cell line. This correlated with... (More)
- The Notch signaling pathway is involved in several lineage commitment and differentiation events. One of these is fate determination of the common lymphoid progenitor, promoting T-cell development at the expense of B-cell differentiation. It has been suggested that this process relies on Notch's ability to inhibit E proteins, which are crucial for early B-cell development. Here, we report that Notch signaling also modulates the function of the transcription factor, early B-cell factor (EBF). Transient transfection of intracellular Notch1 (Notch1-IC) into a pre-B cell line resulted in the down-regulation of EBF-regulated promoters and diminished the capacity of EBF to activate these promoters in an epithelial cell line. This correlated with a reduction in the ability of EBF to bind DNA. Ligand-induced stimulation of endogenous Notch receptors with Delta4 mimicked the activity of Notch1-IC toward EBF. These data suggest that Notch signaling may affect B- versus T-lineage commitment by the targeting of both EBF and E2A. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/225098
- author
- Smith, Emma LU ; Akerblad, P ; Kadesch, T ; Axelson, Håkan LU and Sigvardsson, Mikael LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Blood
- volume
- 106
- issue
- 6
- pages
- 1995 - 2001
- publisher
- American Society of Hematology
- external identifiers
-
- pmid:15920012
- wos:000231817400026
- scopus:24744442149
- ISSN
- 1528-0020
- DOI
- 10.1182/blood-2004-12-4744
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Hematopoietic Stem Cell Laboratory (013022012), Molecular Tumour Biology (013017540) Department affilation moved from v1000583 (Molecular Tumour Biology) to v1000562 (Department of Translational Medicine) on 2016-01-18 14:41:48.
- id
- 3d954f21-7901-4f3b-a44f-86fcd0a18cfb (old id 225098)
- date added to LUP
- 2016-04-01 12:13:45
- date last changed
- 2022-04-21 04:31:26
@article{3d954f21-7901-4f3b-a44f-86fcd0a18cfb, abstract = {{The Notch signaling pathway is involved in several lineage commitment and differentiation events. One of these is fate determination of the common lymphoid progenitor, promoting T-cell development at the expense of B-cell differentiation. It has been suggested that this process relies on Notch's ability to inhibit E proteins, which are crucial for early B-cell development. Here, we report that Notch signaling also modulates the function of the transcription factor, early B-cell factor (EBF). Transient transfection of intracellular Notch1 (Notch1-IC) into a pre-B cell line resulted in the down-regulation of EBF-regulated promoters and diminished the capacity of EBF to activate these promoters in an epithelial cell line. This correlated with a reduction in the ability of EBF to bind DNA. Ligand-induced stimulation of endogenous Notch receptors with Delta4 mimicked the activity of Notch1-IC toward EBF. These data suggest that Notch signaling may affect B- versus T-lineage commitment by the targeting of both EBF and E2A.}}, author = {{Smith, Emma and Akerblad, P and Kadesch, T and Axelson, Håkan and Sigvardsson, Mikael}}, issn = {{1528-0020}}, language = {{eng}}, number = {{6}}, pages = {{1995--2001}}, publisher = {{American Society of Hematology}}, series = {{Blood}}, title = {{Inhibition of EBF function by active Notch signaling reveals a novel regulatory pathway in early B-cell development}}, url = {{http://dx.doi.org/10.1182/blood-2004-12-4744}}, doi = {{10.1182/blood-2004-12-4744}}, volume = {{106}}, year = {{2005}}, }