Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Modeling of spectral changes for depth localization of fluorescent inclusion

Svensson, Jenny LU and Andersson-Engels, Stefan LU (2005) In Optics Express 13(11). p.4263-4274
Abstract
We have performed modeling of fluorescence signals from inclusions inside turbid media to investigate the influence of a limited fluorescence contrast and how accurately the depth can be determined by using the spectral information. The depth was determined by forming a ratio of simulated fluorescence intensities at two wavelengths. The results show that it is important to consider the background autofluorescence in determining the depth of a fluorescent inclusion. It is also necessary to know the optical properties of the tissue to obtain the depth. A 20% error in absorption or scattering coefficients yields an error in the determined depth of approximately 2-3 mm (relative error of 10-15%) in a 20 mm thick tissue slab.
Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Optics Express
volume
13
issue
11
pages
4263 - 4274
publisher
Optical Society of America
external identifiers
  • wos:000229534500043
  • scopus:21244469457
ISSN
1094-4087
language
English
LU publication?
yes
id
a0214f6d-85d6-4743-992e-a87481a38304 (old id 238674)
alternative location
http://www.opticsexpress.org/abstract.cfm?id=84168
date added to LUP
2016-04-01 16:43:14
date last changed
2025-10-14 11:42:07
@article{a0214f6d-85d6-4743-992e-a87481a38304,
  abstract     = {{We have performed modeling of fluorescence signals from inclusions inside turbid media to investigate the influence of a limited fluorescence contrast and how accurately the depth can be determined by using the spectral information. The depth was determined by forming a ratio of simulated fluorescence intensities at two wavelengths. The results show that it is important to consider the background autofluorescence in determining the depth of a fluorescent inclusion. It is also necessary to know the optical properties of the tissue to obtain the depth. A 20% error in absorption or scattering coefficients yields an error in the determined depth of approximately 2-3 mm (relative error of 10-15%) in a 20 mm thick tissue slab.}},
  author       = {{Svensson, Jenny and Andersson-Engels, Stefan}},
  issn         = {{1094-4087}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{4263--4274}},
  publisher    = {{Optical Society of America}},
  series       = {{Optics Express}},
  title        = {{Modeling of spectral changes for depth localization of fluorescent inclusion}},
  url          = {{https://lup.lub.lu.se/search/files/4760288/2370794.pdf}},
  volume       = {{13}},
  year         = {{2005}},
}