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Identification and characterization of antigen-specific t-cells in viral infections

Trubach, Darya ; Muleta, Konjit Getachew LU ; Lahl, Katharina LU and Saini, Sunil Kumar (2024) In Methods in Molecular Biology 2813. p.295-308
Abstract

Identification and characterization of CD8+ T-cells is important to determine their role in protecting and clearing viral infections. Here we provide details of the peptide-MHC (pMHC) tetramers-based approach to identify antigen-specific T-cells in human and murine samples. This method provides ex vivo quantifica-tion and functional characterization of T-cells reactive to specific viral antigens derived from CMV and rotavirus in human blood and in murine intestinal lamina propria samples, respectively.

Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
CD8+ T-cells, Cytokine secretion assay, Flow cytometry, Rotavirus, SARS-CoV-2, T-cell epitopes, T-cell staining, Tetramers
host publication
Methods in Molecular Biology
series title
Methods in Molecular Biology
volume
2813
pages
14 pages
publisher
Humana Press
external identifiers
  • scopus:85196598846
  • pmid:38888785
ISSN
1064-3745
1940-6029
DOI
10.1007/978-1-0716-3890-3_19
language
English
LU publication?
yes
additional info
ยด
id
2939ded2-87f9-4799-b238-2fca7a7f47fc
date added to LUP
2024-09-04 15:07:00
date last changed
2024-09-18 16:20:13
@inbook{2939ded2-87f9-4799-b238-2fca7a7f47fc,
  abstract     = {{<p>Identification and characterization of CD8<sup>+</sup> T-cells is important to determine their role in protecting and clearing viral infections. Here we provide details of the peptide-MHC (pMHC) tetramers-based approach to identify antigen-specific T-cells in human and murine samples. This method provides ex vivo quantifica-tion and functional characterization of T-cells reactive to specific viral antigens derived from CMV and rotavirus in human blood and in murine intestinal lamina propria samples, respectively.</p>}},
  author       = {{Trubach, Darya and Muleta, Konjit Getachew and Lahl, Katharina and Saini, Sunil Kumar}},
  booktitle    = {{Methods in Molecular Biology}},
  issn         = {{1064-3745}},
  keywords     = {{CD8+ T-cells; Cytokine secretion assay; Flow cytometry; Rotavirus; SARS-CoV-2; T-cell epitopes; T-cell staining; Tetramers}},
  language     = {{eng}},
  pages        = {{295--308}},
  publisher    = {{Humana Press}},
  series       = {{Methods in Molecular Biology}},
  title        = {{Identification and characterization of antigen-specific t-cells in viral infections}},
  url          = {{http://dx.doi.org/10.1007/978-1-0716-3890-3_19}},
  doi          = {{10.1007/978-1-0716-3890-3_19}},
  volume       = {{2813}},
  year         = {{2024}},
}