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Use of PLL-g-PEG in micro-fluidic devices for localizing selective and specific protein binding

Marie, Rodolphe LU ; Beech, Jason LU ; Voeroes, Janos ; Tegenfeldt, Jonas LU orcid and Höök, Fredrik LU (2006) In Langmuir 22(24). p.10103-10108
Abstract
By utilizing flow-controlled PLL-g-PEG and PLL-g-PEGbiotin modification of predefined regions of a poly-(dimethylsiloxane) (PDMS) micro-fluidic device, with an intentionally chosen large (similar to 1 cm(2)) internal surface area, we report rapid (10 min), highly localized (6 x 10(-6) cm(2)), and specific surface-based protein capture from a sample volume (100 mu L) containing a low amount of protein (160 attomol in pure buffer and 400 attomol in serum). The design criteria for this surface modification were achieved using QCM-D (quartz crystal microbalance with energy dissipation monitoring) of serum protein adsorption onto PLL-g-PEG-modified oxidized PDMS. Equally good, or almost as good, results were obtained for oxidized SU-8, Topas,... (More)
By utilizing flow-controlled PLL-g-PEG and PLL-g-PEGbiotin modification of predefined regions of a poly-(dimethylsiloxane) (PDMS) micro-fluidic device, with an intentionally chosen large (similar to 1 cm(2)) internal surface area, we report rapid (10 min), highly localized (6 x 10(-6) cm(2)), and specific surface-based protein capture from a sample volume (100 mu L) containing a low amount of protein (160 attomol in pure buffer and 400 attomol in serum). The design criteria for this surface modification were achieved using QCM-D (quartz crystal microbalance with energy dissipation monitoring) of serum protein adsorption onto PLL-g-PEG-modified oxidized PDMS. Equally good, or almost as good, results were obtained for oxidized SU-8, Topas, and poly(methyl metacrylate) (PMMA), demonstrating the generic potential of PLL-g-PEG for surface modification in various micro-fluidic applications. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
22
issue
24
pages
10103 - 10108
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000242022100044
  • scopus:33846121311
  • pmid:17107006
ISSN
0743-7463
DOI
10.1021/la060198m
language
English
LU publication?
yes
id
8c0875d3-e6a3-4816-8586-d59bf7dff685 (old id 376914)
date added to LUP
2016-04-01 11:37:28
date last changed
2024-08-20 11:37:36
@article{8c0875d3-e6a3-4816-8586-d59bf7dff685,
  abstract     = {{By utilizing flow-controlled PLL-g-PEG and PLL-g-PEGbiotin modification of predefined regions of a poly-(dimethylsiloxane) (PDMS) micro-fluidic device, with an intentionally chosen large (similar to 1 cm(2)) internal surface area, we report rapid (10 min), highly localized (6 x 10(-6) cm(2)), and specific surface-based protein capture from a sample volume (100 mu L) containing a low amount of protein (160 attomol in pure buffer and 400 attomol in serum). The design criteria for this surface modification were achieved using QCM-D (quartz crystal microbalance with energy dissipation monitoring) of serum protein adsorption onto PLL-g-PEG-modified oxidized PDMS. Equally good, or almost as good, results were obtained for oxidized SU-8, Topas, and poly(methyl metacrylate) (PMMA), demonstrating the generic potential of PLL-g-PEG for surface modification in various micro-fluidic applications.}},
  author       = {{Marie, Rodolphe and Beech, Jason and Voeroes, Janos and Tegenfeldt, Jonas and Höök, Fredrik}},
  issn         = {{0743-7463}},
  language     = {{eng}},
  number       = {{24}},
  pages        = {{10103--10108}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Langmuir}},
  title        = {{Use of PLL-g-PEG in micro-fluidic devices for localizing selective and specific protein binding}},
  url          = {{http://dx.doi.org/10.1021/la060198m}},
  doi          = {{10.1021/la060198m}},
  volume       = {{22}},
  year         = {{2006}},
}