Variations of wettability and protein adsorption on solid siliceous carriers grafted with poly(N-isopropylacrylamide)
(2006) In Journal of Colloid and Interface Science 296(2). p.538-544- Abstract
- Poly(N-isopropylacrylamide), a thermally responsive polymer, was end-grafted to mercaptopropyl derivatives of silica gel, plane glass sheets and glass capillary tubing by free radical polymerization of the monomer in 1,4-dioxane at 100 degrees C. The polymer monolayer attached to the glass carriers provided them with thermally controlled wettability registered by two independent methods: direct measurements of the water contact angle and capillary rise. The water Contact angle changed from 54 +/- 3 degrees to 68 +/- 3 degrees in the temperature range from 20 to 50 degrees C. The polymer grafting to silica gel (pore diameter 100 A, particle size 5 pm) resulted in 15-30-fold reduction in protein adsorption on the carrier at 35 degrees C.... (More)
- Poly(N-isopropylacrylamide), a thermally responsive polymer, was end-grafted to mercaptopropyl derivatives of silica gel, plane glass sheets and glass capillary tubing by free radical polymerization of the monomer in 1,4-dioxane at 100 degrees C. The polymer monolayer attached to the glass carriers provided them with thermally controlled wettability registered by two independent methods: direct measurements of the water contact angle and capillary rise. The water Contact angle changed from 54 +/- 3 degrees to 68 +/- 3 degrees in the temperature range from 20 to 50 degrees C. The polymer grafting to silica gel (pore diameter 100 A, particle size 5 pm) resulted in 15-30-fold reduction in protein adsorption on the carrier at 35 degrees C. Adsorption isotherms of myoglobin indicate completely different characters of the protein adsorption to silica gel and its polyNIPAM-grafted derivative. Cooling of the grafted carrier containing adsorbed myoglobin to 9 degrees C led to a partial release of the protein to the contacting solution, whereas heating of the system to 35 degrees C resulted in reversible binding of the protein. Adsorption of myoglobin on polyNIPAM-coated silica was ca. 2-fold higher at 35 than at 9 degrees C, most probably (c) 2005 Elsevier Inc. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/414790
- author
- Ivanov, Alexander LU ; Ekeroth, J ; Nilsson, Lars LU ; Mattiasson, Bo LU ; Bergenståhl, Björn LU and Galaev, Igor LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- thermoresponsive polymers, silica, grafting, protein adsorption
- in
- Journal of Colloid and Interface Science
- volume
- 296
- issue
- 2
- pages
- 538 - 544
- publisher
- Elsevier
- external identifiers
-
- wos:000236424600019
- scopus:33644996060
- pmid:16243347
- ISSN
- 1095-7103
- DOI
- 10.1016/j.jcis.2005.09.064
- language
- English
- LU publication?
- yes
- id
- e1d2c3b1-6022-4100-b510-38324d166687 (old id 414790)
- date added to LUP
- 2016-04-01 12:18:36
- date last changed
- 2023-11-11 20:27:58
@article{e1d2c3b1-6022-4100-b510-38324d166687,
abstract = {{Poly(N-isopropylacrylamide), a thermally responsive polymer, was end-grafted to mercaptopropyl derivatives of silica gel, plane glass sheets and glass capillary tubing by free radical polymerization of the monomer in 1,4-dioxane at 100 degrees C. The polymer monolayer attached to the glass carriers provided them with thermally controlled wettability registered by two independent methods: direct measurements of the water contact angle and capillary rise. The water Contact angle changed from 54 +/- 3 degrees to 68 +/- 3 degrees in the temperature range from 20 to 50 degrees C. The polymer grafting to silica gel (pore diameter 100 A, particle size 5 pm) resulted in 15-30-fold reduction in protein adsorption on the carrier at 35 degrees C. Adsorption isotherms of myoglobin indicate completely different characters of the protein adsorption to silica gel and its polyNIPAM-grafted derivative. Cooling of the grafted carrier containing adsorbed myoglobin to 9 degrees C led to a partial release of the protein to the contacting solution, whereas heating of the system to 35 degrees C resulted in reversible binding of the protein. Adsorption of myoglobin on polyNIPAM-coated silica was ca. 2-fold higher at 35 than at 9 degrees C, most probably (c) 2005 Elsevier Inc. All rights reserved.}},
author = {{Ivanov, Alexander and Ekeroth, J and Nilsson, Lars and Mattiasson, Bo and Bergenståhl, Björn and Galaev, Igor}},
issn = {{1095-7103}},
keywords = {{thermoresponsive polymers; silica; grafting; protein adsorption}},
language = {{eng}},
number = {{2}},
pages = {{538--544}},
publisher = {{Elsevier}},
series = {{Journal of Colloid and Interface Science}},
title = {{Variations of wettability and protein adsorption on solid siliceous carriers grafted with poly(N-isopropylacrylamide)}},
url = {{http://dx.doi.org/10.1016/j.jcis.2005.09.064}},
doi = {{10.1016/j.jcis.2005.09.064}},
volume = {{296}},
year = {{2006}},
}