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Short and long-range cyclic patterns in flows of DNA solutions in microfluidic obstacle arrays

Ström, Oskar E. LU orcid ; Beech, Jason P. LU and Tegenfeldt, Jonas O. LU orcid (2023) In Lab on a Chip 23. p.1779-1793
Abstract

We observe regular patterns emerging across multiple length scales with high-concentration DNA solutions in microfluidic pillar arrays at low Reynolds numbers and high Deborah numbers. Interacting vortices between pillars lead to long-range order in the form of large travelling waves consisting of DNA at high concentration and extension. Waves are formed in quadratic arrays of pillars, while randomizing the position of the pillar in each unit cell of a quadratic array leads to suppression of the long-range patterns. We find that concentrations exceeding the overlap concentration of the DNA enables the waves, and exploring the behavior of the waves as a function of flow rate, buffer composition, concentration and molecular length, we... (More)

We observe regular patterns emerging across multiple length scales with high-concentration DNA solutions in microfluidic pillar arrays at low Reynolds numbers and high Deborah numbers. Interacting vortices between pillars lead to long-range order in the form of large travelling waves consisting of DNA at high concentration and extension. Waves are formed in quadratic arrays of pillars, while randomizing the position of the pillar in each unit cell of a quadratic array leads to suppression of the long-range patterns. We find that concentrations exceeding the overlap concentration of the DNA enables the waves, and exploring the behavior of the waves as a function of flow rate, buffer composition, concentration and molecular length, we identify elastic effects as central to the origin of the waves. Our work may not only help increase the low throughput that often limits sample processing in microfluidics, it may also provide a platform for further studies of the underlying viscoelastic mechanisms.

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publication status
published
subject
in
Lab on a Chip
volume
23
pages
15 pages
publisher
Royal Society of Chemistry
external identifiers
  • scopus:85148869161
  • pmid:36807458
ISSN
1473-0197
DOI
10.1039/d2lc01051h
language
English
LU publication?
yes
additional info
Funding Information: This research was funded by the European Union within Horizon2020, grant number 634890 (project name BeyondSeq), EuroNanoMed (project name NanoDiaBac), by the Swedish Research council, grant number 2016-05739 and NanoLund, grant numbers p20-2019 and staff01-2020. All device processing was conducted within Lund Nano Lab. Publisher Copyright: © 2023 The Royal Society of Chemistry.
id
417da42d-569e-48d1-a436-e1889a8ae26c
date added to LUP
2023-03-09 19:31:08
date last changed
2024-07-11 17:15:48
@article{417da42d-569e-48d1-a436-e1889a8ae26c,
  abstract     = {{<p>We observe regular patterns emerging across multiple length scales with high-concentration DNA solutions in microfluidic pillar arrays at low Reynolds numbers and high Deborah numbers. Interacting vortices between pillars lead to long-range order in the form of large travelling waves consisting of DNA at high concentration and extension. Waves are formed in quadratic arrays of pillars, while randomizing the position of the pillar in each unit cell of a quadratic array leads to suppression of the long-range patterns. We find that concentrations exceeding the overlap concentration of the DNA enables the waves, and exploring the behavior of the waves as a function of flow rate, buffer composition, concentration and molecular length, we identify elastic effects as central to the origin of the waves. Our work may not only help increase the low throughput that often limits sample processing in microfluidics, it may also provide a platform for further studies of the underlying viscoelastic mechanisms.</p>}},
  author       = {{Ström, Oskar E. and Beech, Jason P. and Tegenfeldt, Jonas O.}},
  issn         = {{1473-0197}},
  language     = {{eng}},
  pages        = {{1779--1793}},
  publisher    = {{Royal Society of Chemistry}},
  series       = {{Lab on a Chip}},
  title        = {{Short and long-range cyclic patterns in flows of DNA solutions in microfluidic obstacle arrays}},
  url          = {{http://dx.doi.org/10.1039/d2lc01051h}},
  doi          = {{10.1039/d2lc01051h}},
  volume       = {{23}},
  year         = {{2023}},
}