Fluorescently labeled xylosides offer insight into the biosynthetic pathways of glycosaminoglycans
(2021) In RSC Advances 11(60). p.38283-38292- Abstract
Five novel xylosides tagged with the fluorescent probe Pacific Blue™ were synthesized and found to act as substrates for β4GalT7, a bottleneck enzyme in the biosynthetic pathways leading to glycosaminoglycans. By confocal microscopy of A549 cells, we showed that the xylosides were taken up by the cells, but did not enter the Golgi apparatus where most of the glycosaminoglycan biosynthesis occurs. Instead, after a possible double galactosylation by β4GalT7 and β3GalT6, the biosynthesis was terminated. We hypothesize this is due to the charge of the fluorescent probe, which is required for fluorescent ability and stability under physiological conditions.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/46822025-20a6-47d1-91a9-b96463ee8ead
- author
- Mastio, Roberto LU ; Frankel Willén, Daniel LU ; Söderlund, Zackarias LU ; Westergren-Thorsson, Gunilla LU ; Manner, Sophie LU ; Tykesson, Emil LU and Ellervik, Ulf LU
- organization
- publishing date
- 2021-11-23
- type
- Contribution to journal
- publication status
- published
- subject
- in
- RSC Advances
- volume
- 11
- issue
- 60
- pages
- 10 pages
- publisher
- Royal Society of Chemistry
- external identifiers
-
- scopus:85119918793
- pmid:35498069
- ISSN
- 2046-2069
- DOI
- 10.1039/d1ra06320k
- language
- English
- LU publication?
- yes
- additional info
- This journal is © The Royal Society of Chemistry.
- id
- 46822025-20a6-47d1-91a9-b96463ee8ead
- date added to LUP
- 2023-08-11 08:09:23
- date last changed
- 2024-08-10 10:20:57
@article{46822025-20a6-47d1-91a9-b96463ee8ead, abstract = {{<p>Five novel xylosides tagged with the fluorescent probe Pacific Blue™ were synthesized and found to act as substrates for β4GalT7, a bottleneck enzyme in the biosynthetic pathways leading to glycosaminoglycans. By confocal microscopy of A549 cells, we showed that the xylosides were taken up by the cells, but did not enter the Golgi apparatus where most of the glycosaminoglycan biosynthesis occurs. Instead, after a possible double galactosylation by β4GalT7 and β3GalT6, the biosynthesis was terminated. We hypothesize this is due to the charge of the fluorescent probe, which is required for fluorescent ability and stability under physiological conditions.</p>}}, author = {{Mastio, Roberto and Frankel Willén, Daniel and Söderlund, Zackarias and Westergren-Thorsson, Gunilla and Manner, Sophie and Tykesson, Emil and Ellervik, Ulf}}, issn = {{2046-2069}}, language = {{eng}}, month = {{11}}, number = {{60}}, pages = {{38283--38292}}, publisher = {{Royal Society of Chemistry}}, series = {{RSC Advances}}, title = {{Fluorescently labeled xylosides offer insight into the biosynthetic pathways of glycosaminoglycans}}, url = {{http://dx.doi.org/10.1039/d1ra06320k}}, doi = {{10.1039/d1ra06320k}}, volume = {{11}}, year = {{2021}}, }