O-193 THE SAFECHROM PROJECT - CIRCULATING LUNG-CANCER-RELATED NON-CODING RNAS ARE ASSOCIATED WITH OCCUPATIONAL EXPOSURE TO HEXAVALENT CHROMIUM
(2024) In Occupational Medicine 74(Suppl 1). p.378-379- Abstract
- Hexavalent chromium, Cr(VI), is classified as a Group 1 human carcinogen and causes lung cancer. This study was to investigate associations between occupational exposure to Cr(VI) and lung cancer-related non-coding RNAs.The study included 111 Cr(VI) exposed workers and 72 controls in Sweden, 91.4\smokers. Cr concentration in red blood cells (RBC-Cr) was measured. MicroRNA (miRNA) and long non-coding RNA (lncRNA) were extracted from plasma followed by DNAse treatment, cDNA synthesis, and qPCR using target-specific assays for four miRNAs (miR-142-3p, miR-15b-5p, miR-3940-5p, miR-451a), and three lncRNAs (H19, MALAT1, NORAD).Median RBC-Cr concentrations were significantly higher in the exposed group (0.73 µg/L, 5-95\.51-2.33) compared with... (More)
- Hexavalent chromium, Cr(VI), is classified as a Group 1 human carcinogen and causes lung cancer. This study was to investigate associations between occupational exposure to Cr(VI) and lung cancer-related non-coding RNAs.The study included 111 Cr(VI) exposed workers and 72 controls in Sweden, 91.4\smokers. Cr concentration in red blood cells (RBC-Cr) was measured. MicroRNA (miRNA) and long non-coding RNA (lncRNA) were extracted from plasma followed by DNAse treatment, cDNA synthesis, and qPCR using target-specific assays for four miRNAs (miR-142-3p, miR-15b-5p, miR-3940-5p, miR-451a), and three lncRNAs (H19, MALAT1, NORAD).Median RBC-Cr concentrations were significantly higher in the exposed group (0.73 µg/L, 5-95\.51-2.33) compared with controls (0.53 µg/L, 0.42-0.72). Plasma relative expression levels of all miRNAs, MALAT1 and NORAD were significantly lower in the exposed workers compared with controls. In the exposed group, significant negative correlations (rS=-0.19 - -0.27) were found between RBC-Cr and miR-142-3p, miR-15b-5p, miR-451a and NORAD. NORAD correlated with miR-142-3p (rS=0.34) and miR-15b-5p (rS=0.28). In multivariate regression models adjusting for confounders, expression of the miRNAs, MALAT1 and NORAD was still significantly lower in the exposed group compared with controls, and the expression decreased with increasing RBC-Cr.Cr(VI) exposure was associated in a dose-response manner with differential expression of circulating non-coding RNAs in exposed workers, which suggests non-coding RNAs as potential toxicological biomarkers for Cr(VI). Relations between miRNAs and lncRNAs suggest that they participate in the same lncRNA-miRNA-mRNA regulatory axes, which may play important roles in Cr(VI) carcinogenesis.Occupational Cr(VI) exposure may impact circulating lung-cancer-related non-coding RNAs expression. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4a94897b-485d-421d-829b-5971cdf2eb26
- author
- Jiang, Zheshun LU ; Person, Romane ; Abushanab, Heam ; Pineda, Daniela LU ; Lundh, Thomas LU ; Engfeldt, Malin LU and Broberg, Karin LU
- organization
-
- Genetic Occupational and Environmental Medicine (research group)
- Applied Mass Spectrometry in Environmental Medicine (research group)
- Metalund
- Occupational and Environmental Dermatology (research group)
- EpiHealth: Epidemiology for Health
- LTH Profile Area: Aerosols
- LUCC: Lund University Cancer Centre
- publishing date
- 2024-07-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Occupational Medicine
- volume
- 74
- issue
- Suppl 1
- article number
- ID:23712
- pages
- 378 - 379
- publisher
- Oxford University Press
- ISSN
- 0962-7480
- DOI
- 10.1093/occmed/kqae023.0956
- language
- English
- LU publication?
- yes
- id
- 4a94897b-485d-421d-829b-5971cdf2eb26
- alternative location
- https://doi.org/10.1093/occmed/kqae023.0956
- date added to LUP
- 2024-07-08 10:50:15
- date last changed
- 2024-07-10 07:44:12
@misc{4a94897b-485d-421d-829b-5971cdf2eb26, abstract = {{Hexavalent chromium, Cr(VI), is classified as a Group 1 human carcinogen and causes lung cancer. This study was to investigate associations between occupational exposure to Cr(VI) and lung cancer-related non-coding RNAs.The study included 111 Cr(VI) exposed workers and 72 controls in Sweden, 91.4\smokers. Cr concentration in red blood cells (RBC-Cr) was measured. MicroRNA (miRNA) and long non-coding RNA (lncRNA) were extracted from plasma followed by DNAse treatment, cDNA synthesis, and qPCR using target-specific assays for four miRNAs (miR-142-3p, miR-15b-5p, miR-3940-5p, miR-451a), and three lncRNAs (H19, MALAT1, NORAD).Median RBC-Cr concentrations were significantly higher in the exposed group (0.73 µg/L, 5-95\.51-2.33) compared with controls (0.53 µg/L, 0.42-0.72). Plasma relative expression levels of all miRNAs, MALAT1 and NORAD were significantly lower in the exposed workers compared with controls. In the exposed group, significant negative correlations (rS=-0.19 - -0.27) were found between RBC-Cr and miR-142-3p, miR-15b-5p, miR-451a and NORAD. NORAD correlated with miR-142-3p (rS=0.34) and miR-15b-5p (rS=0.28). In multivariate regression models adjusting for confounders, expression of the miRNAs, MALAT1 and NORAD was still significantly lower in the exposed group compared with controls, and the expression decreased with increasing RBC-Cr.Cr(VI) exposure was associated in a dose-response manner with differential expression of circulating non-coding RNAs in exposed workers, which suggests non-coding RNAs as potential toxicological biomarkers for Cr(VI). Relations between miRNAs and lncRNAs suggest that they participate in the same lncRNA-miRNA-mRNA regulatory axes, which may play important roles in Cr(VI) carcinogenesis.Occupational Cr(VI) exposure may impact circulating lung-cancer-related non-coding RNAs expression.}}, author = {{Jiang, Zheshun and Person, Romane and Abushanab, Heam and Pineda, Daniela and Lundh, Thomas and Engfeldt, Malin and Broberg, Karin}}, issn = {{0962-7480}}, language = {{eng}}, month = {{07}}, note = {{Conference Abstract}}, number = {{Suppl 1}}, pages = {{378--379}}, publisher = {{Oxford University Press}}, series = {{Occupational Medicine}}, title = {{O-193 THE SAFECHROM PROJECT - CIRCULATING LUNG-CANCER-RELATED NON-CODING RNAS ARE ASSOCIATED WITH OCCUPATIONAL EXPOSURE TO HEXAVALENT CHROMIUM}}, url = {{http://dx.doi.org/10.1093/occmed/kqae023.0956}}, doi = {{10.1093/occmed/kqae023.0956}}, volume = {{74}}, year = {{2024}}, }