Label-free separation of leukocyte subpopulations using high throughput multiplex acoustophoresis
(2019) In Lab on a Chip 19(8). p.1406-1416- Abstract
Multiplex separation of mixed cell samples is required in a variety of clinical and research applications. Herein, we present an acoustic microchip with multiple outlets and integrated pre-alignment channel to enable high performance and label-free separation of three different cell or particle fractions simultaneously at high sample throughput. By implementing a new cooling system for rigorous temperature control and minimal acoustic energy losses, we were able to operate the system isothermally and sort suspensions of 3, 5 and 7 μm beads with high efficiencies (>95.4%) and purities (>96.3%) at flow rates up to 500 μL min
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Multiplex separation of mixed cell samples is required in a variety of clinical and research applications. Herein, we present an acoustic microchip with multiple outlets and integrated pre-alignment channel to enable high performance and label-free separation of three different cell or particle fractions simultaneously at high sample throughput. By implementing a new cooling system for rigorous temperature control and minimal acoustic energy losses, we were able to operate the system isothermally and sort suspensions of 3, 5 and 7 μm beads with high efficiencies (>95.4%) and purities (>96.3%) at flow rates up to 500 μL min
-1
corresponding to a throughput of ∼2.5 × 10
6
beads per min. Also, human viable white blood cells were successfully fractionated into lymphocytes, monocytes and granulocytes with high purities of 96.5 ± 1.6%, 71.8 ± 10.1% and 98.8 ± 0.5%, respectively, as well as high efficiencies (96.8 ± 3.3%, 66.7 ± 3.2% and 99.0 ± 0.7%) at flow rates up to 100 μL min
-1
(∼100000 cells per min). By increasing the flow rate up to 300 μL min
-1
(∼300000 cells per min) both lymphocytes and granulocytes were still recovered with high purities (92.8 ± 1.9%, 98.2 ± 1.0%), whereas the monocyte purity decreased to 20.9 ± 10.3%. The proposed isothermal multiplex acoustophoresis platform offers efficient fractionation of complex samples in a label-free and continuous manner at thus far unreached high sample throughput rates.
- author
- Urbansky, Anke LU ; Olm, Franziska LU ; Scheding, Stefan LU ; Laurell, Thomas LU and Lenshof, Andreas LU
- organization
-
- Department of Biomedical Engineering
- NanoLund: Centre for Nanoscience
- Acoustofluidics group (research group)
- StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
- Bone marrow stem cells and cellular therapies (research group)
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
- MultiPark: Multidisciplinary research focused on Parkinson´s disease
- publishing date
- 2019-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Lab on a Chip
- volume
- 19
- issue
- 8
- pages
- 11 pages
- publisher
- Royal Society of Chemistry
- external identifiers
-
- scopus:85064181703
- pmid:30869100
- ISSN
- 1473-0197
- DOI
- 10.1039/c9lc00181f
- language
- English
- LU publication?
- yes
- id
- 4ee3cab5-1138-49fb-be40-488cf1b2db37
- date added to LUP
- 2019-04-25 14:50:39
- date last changed
- 2024-08-06 14:01:20
@article{4ee3cab5-1138-49fb-be40-488cf1b2db37, abstract = {{<p><br> Multiplex separation of mixed cell samples is required in a variety of clinical and research applications. Herein, we present an acoustic microchip with multiple outlets and integrated pre-alignment channel to enable high performance and label-free separation of three different cell or particle fractions simultaneously at high sample throughput. By implementing a new cooling system for rigorous temperature control and minimal acoustic energy losses, we were able to operate the system isothermally and sort suspensions of 3, 5 and 7 μm beads with high efficiencies (>95.4%) and purities (>96.3%) at flow rates up to 500 μL min <br> <sup>-1</sup><br> corresponding to a throughput of ∼2.5 × 10 <br> <sup>6</sup><br> beads per min. Also, human viable white blood cells were successfully fractionated into lymphocytes, monocytes and granulocytes with high purities of 96.5 ± 1.6%, 71.8 ± 10.1% and 98.8 ± 0.5%, respectively, as well as high efficiencies (96.8 ± 3.3%, 66.7 ± 3.2% and 99.0 ± 0.7%) at flow rates up to 100 μL min <br> <sup>-1</sup><br> (∼100000 cells per min). By increasing the flow rate up to 300 μL min <br> <sup>-1</sup><br> (∼300000 cells per min) both lymphocytes and granulocytes were still recovered with high purities (92.8 ± 1.9%, 98.2 ± 1.0%), whereas the monocyte purity decreased to 20.9 ± 10.3%. The proposed isothermal multiplex acoustophoresis platform offers efficient fractionation of complex samples in a label-free and continuous manner at thus far unreached high sample throughput rates. <br> </p>}}, author = {{Urbansky, Anke and Olm, Franziska and Scheding, Stefan and Laurell, Thomas and Lenshof, Andreas}}, issn = {{1473-0197}}, language = {{eng}}, month = {{01}}, number = {{8}}, pages = {{1406--1416}}, publisher = {{Royal Society of Chemistry}}, series = {{Lab on a Chip}}, title = {{Label-free separation of leukocyte subpopulations using high throughput multiplex acoustophoresis}}, url = {{http://dx.doi.org/10.1039/c9lc00181f}}, doi = {{10.1039/c9lc00181f}}, volume = {{19}}, year = {{2019}}, }