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Photocatalytic Degradation of Bacterial Lipopolysaccharides by Peptide-Coated TiO2 Nanoparticles

Caselli, Lucrezia LU ; Du, Guanqun LU ; Micciulla, Samantha ; Traini, Tanja ; Sebastiani, Federica LU ; Diedrichsen, Ragna Guldsmed ; Köhler, Sebastian LU ; Skoda, Maximilian W.A. ; van der Plas, Mariena J.A. LU and Malmsten, Martin LU (2024) In ACS Applied Materials and Interfaces 16(44). p.60056-60069
Abstract

In this study, we report the degradation of smooth and rough lipopolysaccharides (LPS) from Gram-negative bacteria and of lipoteichoic acid (LTA) from Gram-positive bacteria by peptide-coated TiO2 nanoparticles (TiO2 NPs). While bare TiO2 NPs displayed minor binding to both LPS and LTA, coating TiO2 NPs with the antimicrobial peptide LL-37 dramatically increased the level of binding to both LPS and LTA, decorating these uniformly. Importantly, peptide coating did not suppress reactive oxygen species generation of TiO2 NPs; hence, UV illumination triggered pronounced degradation of LPS and LTA by peptide-coated TiO2 NPs. Structural consequences of oxidative degradation... (More)

In this study, we report the degradation of smooth and rough lipopolysaccharides (LPS) from Gram-negative bacteria and of lipoteichoic acid (LTA) from Gram-positive bacteria by peptide-coated TiO2 nanoparticles (TiO2 NPs). While bare TiO2 NPs displayed minor binding to both LPS and LTA, coating TiO2 NPs with the antimicrobial peptide LL-37 dramatically increased the level of binding to both LPS and LTA, decorating these uniformly. Importantly, peptide coating did not suppress reactive oxygen species generation of TiO2 NPs; hence, UV illumination triggered pronounced degradation of LPS and LTA by peptide-coated TiO2 NPs. Structural consequences of oxidative degradation were examined by neutron reflectometry for smooth LPS, showing that degradation occurred preferentially in its outer O-antigen tails. Furthermore, cryo-TEM and light scattering showed lipopolysaccharide fragments resulting from degradation to be captured by the NP/lipopolysaccharide coaggregates. The capacity of LL-37-TiO2 NPs to capture and degrade LPS and LTA was demonstrated to be of importance for their ability to suppress lipopolysaccharide-induced activation in human monocytes at simultaneously low toxicity. Together, these results suggest that peptide-coated photocatalytic NPs offer opportunities for the confinement of infection and inflammation.

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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
antimicrobial peptide, lipopolysaccharide, oxidation, photocatalysis, TiO
in
ACS Applied Materials and Interfaces
volume
16
issue
44
pages
14 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • scopus:85207537877
  • pmid:39443826
ISSN
1944-8244
DOI
10.1021/acsami.4c15706
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2024 The Authors. Published by American Chemical Society.
id
53633887-3e23-4f34-8dfd-03a14947da8e
date added to LUP
2024-12-17 11:20:38
date last changed
2025-07-16 17:12:50
@article{53633887-3e23-4f34-8dfd-03a14947da8e,
  abstract     = {{<p>In this study, we report the degradation of smooth and rough lipopolysaccharides (LPS) from Gram-negative bacteria and of lipoteichoic acid (LTA) from Gram-positive bacteria by peptide-coated TiO<sub>2</sub> nanoparticles (TiO<sub>2</sub> NPs). While bare TiO<sub>2</sub> NPs displayed minor binding to both LPS and LTA, coating TiO<sub>2</sub> NPs with the antimicrobial peptide LL-37 dramatically increased the level of binding to both LPS and LTA, decorating these uniformly. Importantly, peptide coating did not suppress reactive oxygen species generation of TiO<sub>2</sub> NPs; hence, UV illumination triggered pronounced degradation of LPS and LTA by peptide-coated TiO<sub>2</sub> NPs. Structural consequences of oxidative degradation were examined by neutron reflectometry for smooth LPS, showing that degradation occurred preferentially in its outer O-antigen tails. Furthermore, cryo-TEM and light scattering showed lipopolysaccharide fragments resulting from degradation to be captured by the NP/lipopolysaccharide coaggregates. The capacity of LL-37-TiO<sub>2</sub> NPs to capture and degrade LPS and LTA was demonstrated to be of importance for their ability to suppress lipopolysaccharide-induced activation in human monocytes at simultaneously low toxicity. Together, these results suggest that peptide-coated photocatalytic NPs offer opportunities for the confinement of infection and inflammation.</p>}},
  author       = {{Caselli, Lucrezia and Du, Guanqun and Micciulla, Samantha and Traini, Tanja and Sebastiani, Federica and Diedrichsen, Ragna Guldsmed and Köhler, Sebastian and Skoda, Maximilian W.A. and van der Plas, Mariena J.A. and Malmsten, Martin}},
  issn         = {{1944-8244}},
  keywords     = {{antimicrobial peptide; lipopolysaccharide; oxidation; photocatalysis; TiO}},
  language     = {{eng}},
  month        = {{11}},
  number       = {{44}},
  pages        = {{60056--60069}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{ACS Applied Materials and Interfaces}},
  title        = {{Photocatalytic Degradation of Bacterial Lipopolysaccharides by Peptide-Coated TiO<sub>2</sub> Nanoparticles}},
  url          = {{http://dx.doi.org/10.1021/acsami.4c15706}},
  doi          = {{10.1021/acsami.4c15706}},
  volume       = {{16}},
  year         = {{2024}},
}