Construction of a self-calibrating ratiometric SERS platform based on nanoenzyme-mediated antagonistic Raman signal for determination of total antioxidant capacity
Wang, Xu ; Ding, Jiacheng ; Che, Yandong ; Pullerits, Tõnu LU
; Li, Wenjie
; Yang, Yanqiu
and Song, Peng
(2027)
In Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
365.
- Abstract
In response to the limitations of traditional total antioxidant capacity (TAC) determination methods—namely limited sensitivity and weak anti-interference ability—as well as the reliance on toxic hydrogen peroxide in conventional nanozyme-based sensing systems, silver nanoparticles (HA-AgNPs) with enzyme-mimicking catalytic activity were synthesized using hydroxylamine hydrochloride as the reducing agent. These HA-AgNPs were further integrated with the ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) substrate to construct a dual-site ratiometric sensing platform based on surface-enhanced Raman scattering (SERS). Experimental results demonstrated that HA-AgNPs efficiently catalyzed the oxidation of ABTS using dissolved... (More)
In response to the limitations of traditional total antioxidant capacity (TAC) determination methods—namely limited sensitivity and weak anti-interference ability—as well as the reliance on toxic hydrogen peroxide in conventional nanozyme-based sensing systems, silver nanoparticles (HA-AgNPs) with enzyme-mimicking catalytic activity were synthesized using hydroxylamine hydrochloride as the reducing agent. These HA-AgNPs were further integrated with the ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) substrate to construct a dual-site ratiometric sensing platform based on surface-enhanced Raman scattering (SERS). Experimental results demonstrated that HA-AgNPs efficiently catalyzed the oxidation of ABTS using dissolved oxygen from air, eliminating the requirement for externally supplied hydrogen peroxide. The resulting oxidized ABTS (oxABTS) generated a strong Raman signal at 1405 cm−1. In contrast, the antioxidant substances were capable of reducing oxABTS, resulting in a concurrent decrease in the 1405 cm−1 signal intensity and a synchronous enhancement of the 1601 cm−1 Raman band, resulting in mutually antagonistic Raman signals. Using this reverse correlation between the two characteristic peaks, a ratiometric quantitative detection method was established. The system exhibited a linear response toward ascorbic acid over a concentration range of 10−9 to 10−5 mol/L, with a limit of detection as low as 0.1 nmol/L. Overall, the developed hydrogen peroxide-free nanozyme-based SERS sensing system is simple to operate and highly sensitive, providing a promising approach for rapid screening of total antioxidant capacity in food samples.
(Less)
- author
- Wang, Xu
; Ding, Jiacheng
; Che, Yandong
; Pullerits, Tõnu
LU
; Li, Wenjie
; Yang, Yanqiu
and Song, Peng
- organization
- publishing date
- 2027-01-15
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Enzyme-like oxidase activity, Opposing Raman signal, SERS, Total antioxidant capacity
- in
- Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
- volume
- 365
- article number
- 128693
- pages
- 10 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:105049169027
- pmid:42700641
- ISSN
- 1386-1425
- DOI
- 10.1016/j.saa.2026.128693
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2026 Elsevier B.V.
- id
- 73db9699-00cb-4cac-890b-684ef508b2b4
- date added to LUP
- 2026-09-29 15:29:39
- date last changed
- 2026-10-12 09:30:15
@article{73db9699-00cb-4cac-890b-684ef508b2b4,
abstract = {{<p>In response to the limitations of traditional total antioxidant capacity (TAC) determination methods—namely limited sensitivity and weak anti-interference ability—as well as the reliance on toxic hydrogen peroxide in conventional nanozyme-based sensing systems, silver nanoparticles (HA-AgNPs) with enzyme-mimicking catalytic activity were synthesized using hydroxylamine hydrochloride as the reducing agent. These HA-AgNPs were further integrated with the ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) substrate to construct a dual-site ratiometric sensing platform based on surface-enhanced Raman scattering (SERS). Experimental results demonstrated that HA-AgNPs efficiently catalyzed the oxidation of ABTS using dissolved oxygen from air, eliminating the requirement for externally supplied hydrogen peroxide. The resulting oxidized ABTS (oxABTS) generated a strong Raman signal at 1405 cm<sup>−1</sup>. In contrast, the antioxidant substances were capable of reducing oxABTS, resulting in a concurrent decrease in the 1405 cm<sup>−1</sup> signal intensity and a synchronous enhancement of the 1601 cm<sup>−1</sup> Raman band, resulting in mutually antagonistic Raman signals. Using this reverse correlation between the two characteristic peaks, a ratiometric quantitative detection method was established. The system exhibited a linear response toward ascorbic acid over a concentration range of 10<sup>−9</sup> to 10<sup>−5</sup> mol/L, with a limit of detection as low as 0.1 nmol/L. Overall, the developed hydrogen peroxide-free nanozyme-based SERS sensing system is simple to operate and highly sensitive, providing a promising approach for rapid screening of total antioxidant capacity in food samples.</p>}},
author = {{Wang, Xu and Ding, Jiacheng and Che, Yandong and Pullerits, Tõnu and Li, Wenjie and Yang, Yanqiu and Song, Peng}},
issn = {{1386-1425}},
keywords = {{Enzyme-like oxidase activity; Opposing Raman signal; SERS; Total antioxidant capacity}},
language = {{eng}},
month = {{01}},
publisher = {{Elsevier}},
series = {{Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy}},
title = {{Construction of a self-calibrating ratiometric SERS platform based on nanoenzyme-mediated antagonistic Raman signal for determination of total antioxidant capacity}},
url = {{http://dx.doi.org/10.1016/j.saa.2026.128693}},
doi = {{10.1016/j.saa.2026.128693}},
volume = {{365}},
year = {{2027}},
}