CD169+ Macrophages in Primary Breast Tumors Associate with Tertiary Lymphoid Structures, Tregs and a Worse Prognosis for Patients with Advanced Breast Cancer
(2023) In Cancers 15(4).- Abstract
The presence of CD169+ macrophages in the draining lymph nodes of cancer patients is, for unknown reasons, associated with a beneficial prognosis. We here investigated the prognostic impact of tumor-infiltrating CD169+ macrophages in primary tumors (PTs) and their spatial relation to tumor-infiltrating B and T cells. Using two breast cancer patient cohorts, we show that CD169+ macrophages were spatially associated with the presence of B and T cell tertiary lymphoid-like structures (TLLSs) in both PTs and lymph node metastases (LNMs). While co-infiltration of CD169+/TLLS in PTs correlated with a worse prognosis, the opposite was found when present in LNMs. RNA sequencing of breast tumors... (More)
The presence of CD169+ macrophages in the draining lymph nodes of cancer patients is, for unknown reasons, associated with a beneficial prognosis. We here investigated the prognostic impact of tumor-infiltrating CD169+ macrophages in primary tumors (PTs) and their spatial relation to tumor-infiltrating B and T cells. Using two breast cancer patient cohorts, we show that CD169+ macrophages were spatially associated with the presence of B and T cell tertiary lymphoid-like structures (TLLSs) in both PTs and lymph node metastases (LNMs). While co-infiltration of CD169+/TLLS in PTs correlated with a worse prognosis, the opposite was found when present in LNMs. RNA sequencing of breast tumors further confirmed that SIGLEC1 (CD169) expression was associated with mature tertiary lymphoid structure (TLS), and Treg and Breg signatures. We propose that the negative prognostic value related to CD169+ macrophages in PTs is a consequence of an immunosuppressive tumor environment rich in TLSs, Tregs and Bregs.
(Less)
- author
- Briem, Oscar
LU
; Källberg, Eva
LU
; Kimbung, Siker
LU
; Veerla, Srinivas
LU
; Stenström, Jenny ; Hatschek, Thomas ; Hagerling, Catharina LU ; Hedenfalk, Ingrid LU
and Leandersson, Karin LU
- organization
-
- Cancer Immunology, Malmö (research group)
- LUCC: Lund University Cancer Centre
- Breast/ovarian cancer
- Breast cancer prevention & intervention (research group)
- Breastcancer
- Breast/lungcancer
- Breast and Ovarian Cancer Genomics (research group)
- Division of Translational Cancer Research
- Pathways of cancer cell evolution (research group)
- Stem Cell Center
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- B, breast cancer, CD169, lymph node, macrophage, T, TLS
- in
- Cancers
- volume
- 15
- issue
- 4
- article number
- 1262
- publisher
- MDPI AG
- external identifiers
-
- pmid:36831605
- scopus:85149113931
- ISSN
- 2072-6694
- DOI
- 10.3390/cancers15041262
- language
- English
- LU publication?
- yes
- id
- 78a25819-d7bb-4871-97dd-cbbd18aa5836
- date added to LUP
- 2023-03-20 12:24:19
- date last changed
- 2024-08-09 05:31:23
@article{78a25819-d7bb-4871-97dd-cbbd18aa5836, abstract = {{<p>The presence of CD169<sup>+</sup> macrophages in the draining lymph nodes of cancer patients is, for unknown reasons, associated with a beneficial prognosis. We here investigated the prognostic impact of tumor-infiltrating CD169<sup>+</sup> macrophages in primary tumors (PTs) and their spatial relation to tumor-infiltrating B and T cells. Using two breast cancer patient cohorts, we show that CD169<sup>+</sup> macrophages were spatially associated with the presence of B and T cell tertiary lymphoid-like structures (TLLSs) in both PTs and lymph node metastases (LNMs). While co-infiltration of CD169<sup>+</sup>/TLLS in PTs correlated with a worse prognosis, the opposite was found when present in LNMs. RNA sequencing of breast tumors further confirmed that SIGLEC1 (CD169) expression was associated with mature tertiary lymphoid structure (TLS), and T<sub>reg</sub> and B<sub>reg</sub> signatures. We propose that the negative prognostic value related to CD169<sup>+</sup> macrophages in PTs is a consequence of an immunosuppressive tumor environment rich in TLSs, T<sub>regs</sub> and B<sub>regs</sub>.</p>}}, author = {{Briem, Oscar and Källberg, Eva and Kimbung, Siker and Veerla, Srinivas and Stenström, Jenny and Hatschek, Thomas and Hagerling, Catharina and Hedenfalk, Ingrid and Leandersson, Karin}}, issn = {{2072-6694}}, keywords = {{B; breast cancer; CD169; lymph node; macrophage; T; TLS}}, language = {{eng}}, number = {{4}}, publisher = {{MDPI AG}}, series = {{Cancers}}, title = {{CD169<sup>+</sup> Macrophages in Primary Breast Tumors Associate with Tertiary Lymphoid Structures, T<sub>regs</sub> and a Worse Prognosis for Patients with Advanced Breast Cancer}}, url = {{http://dx.doi.org/10.3390/cancers15041262}}, doi = {{10.3390/cancers15041262}}, volume = {{15}}, year = {{2023}}, }