Perfluorooctanesulfonic acid modulates barrier function and systemic T-cell homeostasis during intestinal inflammation
(2021) In DMM Disease Models and Mechanisms 14(12). p.1-11- Abstract
The intestinal epithelium is continuously exposed to deleterious environmental factors that might cause aberrant immune responses leading to inflammatory disorders. However, what environmental factors might contribute to disease are poorly understood. Here, to overcome the lack of in vivo models suitable for screening of environmental factors, we used zebrafish reporters of intestinal inflammation. Using zebrafish, we interrogated the immunomodulatory effects of polyfluoroalkyl substances, which have been positively associated with ulcerative colitis incidence. Exposure to perfluorooctanesulfonic acid (PFOS) during 2,4,6-trinitro-benzene sulfonic acid (TNBS)-induced inflammation enhanced the expression of proinflammatory cytokines as... (More)
The intestinal epithelium is continuously exposed to deleterious environmental factors that might cause aberrant immune responses leading to inflammatory disorders. However, what environmental factors might contribute to disease are poorly understood. Here, to overcome the lack of in vivo models suitable for screening of environmental factors, we used zebrafish reporters of intestinal inflammation. Using zebrafish, we interrogated the immunomodulatory effects of polyfluoroalkyl substances, which have been positively associated with ulcerative colitis incidence. Exposure to perfluorooctanesulfonic acid (PFOS) during 2,4,6-trinitro-benzene sulfonic acid (TNBS)-induced inflammation enhanced the expression of proinflammatory cytokines as well as neutrophil recruitment to the intestine of zebrafish larvae, which was validated in the TNBS-induced colitis mouse model. Moreover, PFOS exposure in mice undergoing colitis resulted in neutrophil-dependent increased intestinal permeability and enhanced PFOS translocation into the circulation. This was associated with a neutrophil-dependent expansion of systemic CD4+ T cells. Thus, our results indicate that PFOS worsens inflammation-induced intestinal damage with disruption of T-cell homeostasis beyond the gut and provides a novel in vivo toolbox to screen for pollutants affecting intestinal homeostasis.
(Less)
- author
- Diaz, Oscar E. ; Sorini, Chiara ; Morales, Rodrigo A. ; Luo, Xinxin ; Frede, Annika ; Krais, Annette M. LU ; Chávez, Myra N. ; Wincent, Emma ; Das, Srustidhar and Villablanca, Eduardo J.
- organization
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Colitis, Experimental models, Inflammation, Pollutants, T cell
- in
- DMM Disease Models and Mechanisms
- volume
- 14
- issue
- 12
- article number
- dmm049104
- pages
- 1 - 11
- publisher
- The Company of Biologists Ltd
- external identifiers
-
- scopus:85121980839
- pmid:34792120
- pmid:34792120
- ISSN
- 1754-8411
- DOI
- 10.1242/dmm.049104
- language
- English
- LU publication?
- yes
- id
- 7cb94583-1e62-4de5-9d58-8d2e4c151197
- date added to LUP
- 2022-01-24 20:01:15
- date last changed
- 2024-08-11 05:11:40
@article{7cb94583-1e62-4de5-9d58-8d2e4c151197, abstract = {{<p>The intestinal epithelium is continuously exposed to deleterious environmental factors that might cause aberrant immune responses leading to inflammatory disorders. However, what environmental factors might contribute to disease are poorly understood. Here, to overcome the lack of in vivo models suitable for screening of environmental factors, we used zebrafish reporters of intestinal inflammation. Using zebrafish, we interrogated the immunomodulatory effects of polyfluoroalkyl substances, which have been positively associated with ulcerative colitis incidence. Exposure to perfluorooctanesulfonic acid (PFOS) during 2,4,6-trinitro-benzene sulfonic acid (TNBS)-induced inflammation enhanced the expression of proinflammatory cytokines as well as neutrophil recruitment to the intestine of zebrafish larvae, which was validated in the TNBS-induced colitis mouse model. Moreover, PFOS exposure in mice undergoing colitis resulted in neutrophil-dependent increased intestinal permeability and enhanced PFOS translocation into the circulation. This was associated with a neutrophil-dependent expansion of systemic CD4<sup>+</sup> T cells. Thus, our results indicate that PFOS worsens inflammation-induced intestinal damage with disruption of T-cell homeostasis beyond the gut and provides a novel in vivo toolbox to screen for pollutants affecting intestinal homeostasis.</p>}}, author = {{Diaz, Oscar E. and Sorini, Chiara and Morales, Rodrigo A. and Luo, Xinxin and Frede, Annika and Krais, Annette M. and Chávez, Myra N. and Wincent, Emma and Das, Srustidhar and Villablanca, Eduardo J.}}, issn = {{1754-8411}}, keywords = {{Colitis; Experimental models; Inflammation; Pollutants; T cell}}, language = {{eng}}, number = {{12}}, pages = {{1--11}}, publisher = {{The Company of Biologists Ltd}}, series = {{DMM Disease Models and Mechanisms}}, title = {{Perfluorooctanesulfonic acid modulates barrier function and systemic T-cell homeostasis during intestinal inflammation}}, url = {{http://dx.doi.org/10.1242/dmm.049104}}, doi = {{10.1242/dmm.049104}}, volume = {{14}}, year = {{2021}}, }