Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis
(2018) In Journal of Experimental Medicine 215(7). p.1947-1963- Abstract
To understand the developmental trajectories in early lymphocyte differentiation, we identified differentially expressed surface markers on lineage-negative lymphoid progenitors (LPs). Single-cell polymerase chain reaction experiments allowed us to link surface marker expression to that of lineage-associated transcription factors (TFs) and identify GFRA2 and BST1 as markers of early B cells. Functional analyses in vitro and in vivo as well as single-cell gene expression analyses supported that surface expression of these proteins defined distinct subpopulations that include cells from both the classical common LPs (CLPs) and Fraction A compartments. The formation of the GFRA2-expressing stages of development depended on the TF EBF1,... (More)
To understand the developmental trajectories in early lymphocyte differentiation, we identified differentially expressed surface markers on lineage-negative lymphoid progenitors (LPs). Single-cell polymerase chain reaction experiments allowed us to link surface marker expression to that of lineage-associated transcription factors (TFs) and identify GFRA2 and BST1 as markers of early B cells. Functional analyses in vitro and in vivo as well as single-cell gene expression analyses supported that surface expression of these proteins defined distinct subpopulations that include cells from both the classical common LPs (CLPs) and Fraction A compartments. The formation of the GFRA2-expressing stages of development depended on the TF EBF1, critical both for the activation of stage-specific target genes and modulation of the epigenetic landscape. Our data show that consecutive expression of Ly6D, GFRA2, and BST1 defines a developmental trajectory linking the CLP to the CD19+ progenitor compartment.
(Less)
- author
- organization
- publishing date
- 2018-07-02
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Experimental Medicine
- volume
- 215
- issue
- 7
- pages
- 17 pages
- publisher
- Rockefeller University Press
- external identifiers
-
- scopus:85049460005
- pmid:29899037
- ISSN
- 1540-9538
- DOI
- 10.1084/jem.20171384
- language
- English
- LU publication?
- yes
- id
- 7f61afe1-1a43-4086-a076-0c98cba9a9fc
- date added to LUP
- 2018-08-24 14:57:44
- date last changed
- 2024-09-17 01:26:42
@article{7f61afe1-1a43-4086-a076-0c98cba9a9fc, abstract = {{<p>To understand the developmental trajectories in early lymphocyte differentiation, we identified differentially expressed surface markers on lineage-negative lymphoid progenitors (LPs). Single-cell polymerase chain reaction experiments allowed us to link surface marker expression to that of lineage-associated transcription factors (TFs) and identify GFRA2 and BST1 as markers of early B cells. Functional analyses in vitro and in vivo as well as single-cell gene expression analyses supported that surface expression of these proteins defined distinct subpopulations that include cells from both the classical common LPs (CLPs) and Fraction A compartments. The formation of the GFRA2-expressing stages of development depended on the TF EBF1, critical both for the activation of stage-specific target genes and modulation of the epigenetic landscape. Our data show that consecutive expression of Ly6D, GFRA2, and BST1 defines a developmental trajectory linking the CLP to the CD19+ progenitor compartment.</p>}}, author = {{Jensen, Christina T and Åhsberg, Josefine and Sommarin, Mikael N E and Strid, Tobias and Somasundaram, Rajesh and Okuyama, Kazuki and Ungerbäck, Jonas and Kupari, Jussi and Airaksinen, Matti S and Lang, Stefan and Bryder, David and Soneji, Shamit and Karlsson, Göran and Sigvardsson, Mikael}}, issn = {{1540-9538}}, language = {{eng}}, month = {{07}}, number = {{7}}, pages = {{1947--1963}}, publisher = {{Rockefeller University Press}}, series = {{Journal of Experimental Medicine}}, title = {{Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis}}, url = {{http://dx.doi.org/10.1084/jem.20171384}}, doi = {{10.1084/jem.20171384}}, volume = {{215}}, year = {{2018}}, }