Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

MicroRNAs in Type 2 Diabetes : Focus on MicroRNA Profiling in Islets of Langerhans

Cowan, Elaine LU orcid ; Karagiannopoulos, Alexandros LU orcid and Eliasson, Lena LU orcid (2022) In Methods in molecular biology (Clifton, N.J.) p.113-142
Abstract

Differential expression of microRNAs (miRNAs) is observed in many diseases including type 2 diabetes (T2D). Insulin secretion from pancreatic beta cells is central for the regulation of blood glucose levels and failure to release enough insulin results in hyperglycemia and T2D. The importance in T2D pathogenesis of single miRNAs in beta cells has been described; however, to get the full picture, high-throughput miRNA sequencing is necessary. Here we describe a method using small RNA sequencing, from sample preparation to expression analysis using bioinformatic tools. In the end, a tutorial on differential expression analysis is presented in R using publicly available data.

Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Islets of Langerhans, Beta-cell, Insulin secretion, MicroRNA, Diabetes, Cardiometabolic disease, RNA sequencing, Short noncoding RNA, Expression analyses
host publication
Type-1 Diabetes : Methods and Protocols - Methods and Protocols
series title
Methods in molecular biology (Clifton, N.J.)
editor
Moore, Anna and Wang, Ping
issue
2592
pages
30 pages
publisher
Humana Press
external identifiers
  • pmid:36507989
  • scopus:85144388229
ISSN
1940-6029
ISBN
978-1-0716-2807-2
978-1-0716-2806-5
DOI
10.1007/978-1-0716-2807-2_8
language
English
LU publication?
yes
additional info
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
id
96d9df58-b075-4368-a0e5-4d372da98973
date added to LUP
2022-12-14 10:44:30
date last changed
2024-06-27 11:26:27
@inbook{96d9df58-b075-4368-a0e5-4d372da98973,
  abstract     = {{<p>Differential expression of microRNAs (miRNAs) is observed in many diseases including type 2 diabetes (T2D). Insulin secretion from pancreatic beta cells is central for the regulation of blood glucose levels and failure to release enough insulin results in hyperglycemia and T2D. The importance in T2D pathogenesis of single miRNAs in beta cells has been described; however, to get the full picture, high-throughput miRNA sequencing is necessary. Here we describe a method using small RNA sequencing, from sample preparation to expression analysis using bioinformatic tools. In the end, a tutorial on differential expression analysis is presented in R using publicly available data.</p>}},
  author       = {{Cowan, Elaine and Karagiannopoulos, Alexandros and Eliasson, Lena}},
  booktitle    = {{Type-1 Diabetes : Methods and Protocols}},
  editor       = {{Moore, Anna and Wang, Ping}},
  isbn         = {{978-1-0716-2807-2}},
  issn         = {{1940-6029}},
  keywords     = {{Islets of Langerhans; Beta-cell; Insulin secretion; MicroRNA; Diabetes; Cardiometabolic disease; RNA sequencing; Short noncoding RNA; Expression analyses}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{2592}},
  pages        = {{113--142}},
  publisher    = {{Humana Press}},
  series       = {{Methods in molecular biology (Clifton, N.J.)}},
  title        = {{MicroRNAs in Type 2 Diabetes : Focus on MicroRNA Profiling in Islets of Langerhans}},
  url          = {{http://dx.doi.org/10.1007/978-1-0716-2807-2_8}},
  doi          = {{10.1007/978-1-0716-2807-2_8}},
  year         = {{2022}},
}