Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Quantitative mass spectrometry analysis of the injured proximal and distal human digital nerve ends

Frostadottir, Drifa LU ; Welinder, Charlotte LU ; Perez, Raquel LU orcid and Dahlin, Lars B. LU orcid (2024) In Frontiers in Molecular Neuroscience 17.
Abstract

Introduction: Proteomic analysis of injured human peripheral nerves, particularly focusing on events occurring in the proximal and distal nerve ends, remains relatively underexplored. This study aimed to investigate the molecular patterns underlying a digital nerve injury, focusing on differences in protein expression between the proximal and distal nerve ends. Methods: A total of 26 human injured digital nerve samples (24 men; 2 women; median age 47 [30–66] years), harvested during primary nerve repair within 48 h post-injury from proximal and distal nerve ends, were analyzed using mass spectrometry. Results: A total of 3,914 proteins were identified, with 127 proteins showing significant differences in abundance between the proximal... (More)

Introduction: Proteomic analysis of injured human peripheral nerves, particularly focusing on events occurring in the proximal and distal nerve ends, remains relatively underexplored. This study aimed to investigate the molecular patterns underlying a digital nerve injury, focusing on differences in protein expression between the proximal and distal nerve ends. Methods: A total of 26 human injured digital nerve samples (24 men; 2 women; median age 47 [30–66] years), harvested during primary nerve repair within 48 h post-injury from proximal and distal nerve ends, were analyzed using mass spectrometry. Results: A total of 3,914 proteins were identified, with 127 proteins showing significant differences in abundance between the proximal and the distal nerve ends. The downregulation of proteins in the distal nerve end was associated with synaptic transmission, autophagy, neurotransmitter regulation, cell adhesion and migration. Conversely, proteins upregulated in the distal nerve end were implicated in cellular stress response, neuromuscular junction stability and muscle contraction, neuronal excitability and neurotransmitter release, synaptic vesicle recycling and axon guidance and angiogenesis. Discussion: Investigation of proteins, with functional annotations analysis, in proximal and the distal ends of human injured digital nerves, revealed dynamic cellular responses aimed at promoting tissue degeneration and restoration, while suppressing non-essential processes.

(Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
digital nerve injury, extracellular matrix, nerve injury pathways, peripheral nerve injury, proteomics, quantitative mass spectrometry, signal transduction
in
Frontiers in Molecular Neuroscience
volume
17
article number
1425780
publisher
Frontiers Media S. A.
external identifiers
  • pmid:39015129
  • scopus:85198647005
ISSN
1662-5099
DOI
10.3389/fnmol.2024.1425780
language
English
LU publication?
yes
id
98451b1f-89cd-4f13-a38e-7022749d478d
date added to LUP
2024-11-28 08:11:32
date last changed
2025-07-11 02:52:01
@article{98451b1f-89cd-4f13-a38e-7022749d478d,
  abstract     = {{<p>Introduction: Proteomic analysis of injured human peripheral nerves, particularly focusing on events occurring in the proximal and distal nerve ends, remains relatively underexplored. This study aimed to investigate the molecular patterns underlying a digital nerve injury, focusing on differences in protein expression between the proximal and distal nerve ends. Methods: A total of 26 human injured digital nerve samples (24 men; 2 women; median age 47 [30–66] years), harvested during primary nerve repair within 48 h post-injury from proximal and distal nerve ends, were analyzed using mass spectrometry. Results: A total of 3,914 proteins were identified, with 127 proteins showing significant differences in abundance between the proximal and the distal nerve ends. The downregulation of proteins in the distal nerve end was associated with synaptic transmission, autophagy, neurotransmitter regulation, cell adhesion and migration. Conversely, proteins upregulated in the distal nerve end were implicated in cellular stress response, neuromuscular junction stability and muscle contraction, neuronal excitability and neurotransmitter release, synaptic vesicle recycling and axon guidance and angiogenesis. Discussion: Investigation of proteins, with functional annotations analysis, in proximal and the distal ends of human injured digital nerves, revealed dynamic cellular responses aimed at promoting tissue degeneration and restoration, while suppressing non-essential processes.</p>}},
  author       = {{Frostadottir, Drifa and Welinder, Charlotte and Perez, Raquel and Dahlin, Lars B.}},
  issn         = {{1662-5099}},
  keywords     = {{digital nerve injury; extracellular matrix; nerve injury pathways; peripheral nerve injury; proteomics; quantitative mass spectrometry; signal transduction}},
  language     = {{eng}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Molecular Neuroscience}},
  title        = {{Quantitative mass spectrometry analysis of the injured proximal and distal human digital nerve ends}},
  url          = {{http://dx.doi.org/10.3389/fnmol.2024.1425780}},
  doi          = {{10.3389/fnmol.2024.1425780}},
  volume       = {{17}},
  year         = {{2024}},
}