Relationship between HMF intake and SMF formation in vivo : An animal and human study
(2017) In Molecular Nutrition and Food Research 61(3).- Abstract
Scope: 5-Hydroxymethylfurfural (HMF) is a furanic compound produced in heat-processed foods by nonenzymatic browning reactions. HMF has been demonstrated to be hepato- and nephrotoxic in animals with a link to its metabolite 5-sulfooxymethylfurfural (SMF). To date little is known about either the formation of SMF from ingested HMF or the formation of DNA adducts in animals or human beings. Methods and results: To assess SMF in vivo formation, we first performed a study in mice treated with high/low doses of oral HMF. We found increased concentrations of SMF in plasma and DNA SMF-adducts in leukocytes, hepatic tissue, and kidneys by means of LC-MS/MS, but no spatial formation in such tissues was observed by MALDI-MS imaging technology... (More)
Scope: 5-Hydroxymethylfurfural (HMF) is a furanic compound produced in heat-processed foods by nonenzymatic browning reactions. HMF has been demonstrated to be hepato- and nephrotoxic in animals with a link to its metabolite 5-sulfooxymethylfurfural (SMF). To date little is known about either the formation of SMF from ingested HMF or the formation of DNA adducts in animals or human beings. Methods and results: To assess SMF in vivo formation, we first performed a study in mice treated with high/low doses of oral HMF. We found increased concentrations of SMF in plasma and DNA SMF-adducts in leukocytes, hepatic tissue, and kidneys by means of LC-MS/MS, but no spatial formation in such tissues was observed by MALDI-MS imaging technology due to low sensitivity. In a second experiment, we measured the exposure to HMF in a Spanish preadolescent population. We analyzed the concentration of HMF metabolites (plasma, urine) and measured, for the first time, the presence of SMF in plasma and DNA SMF-adducts in leukocytes. Conclusion: This study provides the first evidence that oral HMF is readily transformed into SMF in vivo, giving rise to the formation of DNA adducts in a direct relation with HMF intake, both in animals and human beings.
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- author
- Pastoriza de la Cueva, Silvia ; Álvarez, Juana ; Végvári, Ákos LU ; Montilla-Gómez, Javier ; Cruz-López, Olga ; Delgado-Andrade, Cristina and Rufián-Henares, José A.
- organization
- publishing date
- 2017-03
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- 5-Hydroxymethylfurfural, 5-Sulfooxymethylfurfural, Estimated intake, Heat processing, Maillard reaction
- in
- Molecular Nutrition and Food Research
- volume
- 61
- issue
- 3
- article number
- 1600773
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:85009230929
- pmid:27800655
- wos:000398095000010
- ISSN
- 1613-4125
- DOI
- 10.1002/mnfr.201600773
- language
- English
- LU publication?
- yes
- id
- a9eeae59-77cd-4111-bc4f-752865257d90
- date added to LUP
- 2017-02-21 12:02:51
- date last changed
- 2024-06-23 11:49:20
@article{a9eeae59-77cd-4111-bc4f-752865257d90,
abstract = {{<p>Scope: 5-Hydroxymethylfurfural (HMF) is a furanic compound produced in heat-processed foods by nonenzymatic browning reactions. HMF has been demonstrated to be hepato- and nephrotoxic in animals with a link to its metabolite 5-sulfooxymethylfurfural (SMF). To date little is known about either the formation of SMF from ingested HMF or the formation of DNA adducts in animals or human beings. Methods and results: To assess SMF in vivo formation, we first performed a study in mice treated with high/low doses of oral HMF. We found increased concentrations of SMF in plasma and DNA SMF-adducts in leukocytes, hepatic tissue, and kidneys by means of LC-MS/MS, but no spatial formation in such tissues was observed by MALDI-MS imaging technology due to low sensitivity. In a second experiment, we measured the exposure to HMF in a Spanish preadolescent population. We analyzed the concentration of HMF metabolites (plasma, urine) and measured, for the first time, the presence of SMF in plasma and DNA SMF-adducts in leukocytes. Conclusion: This study provides the first evidence that oral HMF is readily transformed into SMF in vivo, giving rise to the formation of DNA adducts in a direct relation with HMF intake, both in animals and human beings.</p>}},
author = {{Pastoriza de la Cueva, Silvia and Álvarez, Juana and Végvári, Ákos and Montilla-Gómez, Javier and Cruz-López, Olga and Delgado-Andrade, Cristina and Rufián-Henares, José A.}},
issn = {{1613-4125}},
keywords = {{5-Hydroxymethylfurfural; 5-Sulfooxymethylfurfural; Estimated intake; Heat processing; Maillard reaction}},
language = {{eng}},
number = {{3}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Molecular Nutrition and Food Research}},
title = {{Relationship between HMF intake and SMF formation in vivo : An animal and human study}},
url = {{http://dx.doi.org/10.1002/mnfr.201600773}},
doi = {{10.1002/mnfr.201600773}},
volume = {{61}},
year = {{2017}},
}