Preparation and Utilization of a Highly Discriminative Absorbent Imprinted with Fetal Hemoglobin
Zhang, Ka LU ; Zhou, Tongchang LU ; Dicko, Cedric LU
; Ye, Lei
LU
and Bülow, Leif
LU
(2024)
In Polymers
16(19).
- Abstract
Development in hemoglobin-based oxygen carriers (HBOCs) that may be used as alternatives to donated blood requires an extensive supply of highly pure hemoglobin (Hb) preparations. Therefore, it is essential to fabricate inexpensive, stable and highly selective absorbents for Hb purification. Molecular imprinting is an attractive technology for preparing such materials for targeted molecular recognition and rapid separations. In this case study, we developed human fetal hemoglobin (HbF)-imprinted polymer beads through the fusion of surface imprinting and Pickering emulsion polymerization. HbF was firstly covalently coupled to silica nanoparticles through its surface-exposed amino groups. The particle-supported HbF molecules were... (More)
Development in hemoglobin-based oxygen carriers (HBOCs) that may be used as alternatives to donated blood requires an extensive supply of highly pure hemoglobin (Hb) preparations. Therefore, it is essential to fabricate inexpensive, stable and highly selective absorbents for Hb purification. Molecular imprinting is an attractive technology for preparing such materials for targeted molecular recognition and rapid separations. In this case study, we developed human fetal hemoglobin (HbF)-imprinted polymer beads through the fusion of surface imprinting and Pickering emulsion polymerization. HbF was firstly covalently coupled to silica nanoparticles through its surface-exposed amino groups. The particle-supported HbF molecules were subsequently employed as templates for the synthesis of molecularly imprinted polymers (MIPs) with high selectivity for Hb. After removing the silica support and HbF, the resulting MIPs underwent equilibrium and kinetic binding experiments with both adult Hb (HbA) and HbF. These surface-imprinted MIPs exhibited excellent selectivity for both HbA and HbF, facilitating the one-step isolation of recombinant Hb from crude biological samples. The saturation capacities of HbA and HbF were found to be 15.4 and 17.1 mg/g polymer, respectively. The present study opens new possibilities for designed resins for tailored protein purification, separation and analysis.
(Less)
- author
- Zhang, Ka
LU
; Zhou, Tongchang
LU
; Dicko, Cedric
LU
; Ye, Lei
LU
and Bülow, Leif
LU
- organization
- publishing date
- 2024-10
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- adult hemoglobin, fetal hemoglobin, molecular imprinting, polymer, purification
- in
- Polymers
- volume
- 16
- issue
- 19
- article number
- 2734
- publisher
- MDPI AG
- external identifiers
-
- pmid:39408446
- scopus:85206499899
- ISSN
- 2073-4360
- DOI
- 10.3390/polym16192734
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2024 by the authors.
- id
- bb828461-9f3d-40e2-8c81-49c44f705099
- date added to LUP
- 2024-12-18 12:21:33
- date last changed
- 2025-07-03 04:37:28
@article{bb828461-9f3d-40e2-8c81-49c44f705099,
abstract = {{<p>Development in hemoglobin-based oxygen carriers (HBOCs) that may be used as alternatives to donated blood requires an extensive supply of highly pure hemoglobin (Hb) preparations. Therefore, it is essential to fabricate inexpensive, stable and highly selective absorbents for Hb purification. Molecular imprinting is an attractive technology for preparing such materials for targeted molecular recognition and rapid separations. In this case study, we developed human fetal hemoglobin (HbF)-imprinted polymer beads through the fusion of surface imprinting and Pickering emulsion polymerization. HbF was firstly covalently coupled to silica nanoparticles through its surface-exposed amino groups. The particle-supported HbF molecules were subsequently employed as templates for the synthesis of molecularly imprinted polymers (MIPs) with high selectivity for Hb. After removing the silica support and HbF, the resulting MIPs underwent equilibrium and kinetic binding experiments with both adult Hb (HbA) and HbF. These surface-imprinted MIPs exhibited excellent selectivity for both HbA and HbF, facilitating the one-step isolation of recombinant Hb from crude biological samples. The saturation capacities of HbA and HbF were found to be 15.4 and 17.1 mg/g polymer, respectively. The present study opens new possibilities for designed resins for tailored protein purification, separation and analysis.</p>}},
author = {{Zhang, Ka and Zhou, Tongchang and Dicko, Cedric and Ye, Lei and Bülow, Leif}},
issn = {{2073-4360}},
keywords = {{adult hemoglobin; fetal hemoglobin; molecular imprinting; polymer; purification}},
language = {{eng}},
number = {{19}},
publisher = {{MDPI AG}},
series = {{Polymers}},
title = {{Preparation and Utilization of a Highly Discriminative Absorbent Imprinted with Fetal Hemoglobin}},
url = {{http://dx.doi.org/10.3390/polym16192734}},
doi = {{10.3390/polym16192734}},
volume = {{16}},
year = {{2024}},
}