Rescue Effect Inherited in Colony Formation Assays Affects Radiation Response
Adrian, Gabriel LU
; Ceberg, Crister
LU
; Carneiro, Ana
LU
and Ekblad, Lars
LU
(2018)
In Radiation Research
189(1).
p.44-52
- Abstract
It is well known that nonirradiated cells can exhibit radiation damage (bystander effect), and recent findings have shown that nonirradiated cells may help protect irradiated cells (rescue effect). These findings call into question the traditional view of radiation response: cells cannot be envisioned as isolated units. Here, we investigated traditional colony formation assays to determine if they also comprise cellular communication affecting the radiation response, using colony formation assays with varying numbers of cells, modulated beam irradiation and media transfer. Our findings showed that surviving fraction gradually increased with increasing number of irradiated cells. Specifically, for DU-145 human prostate cancer cells,... (More)
It is well known that nonirradiated cells can exhibit radiation damage (bystander effect), and recent findings have shown that nonirradiated cells may help protect irradiated cells (rescue effect). These findings call into question the traditional view of radiation response: cells cannot be envisioned as isolated units. Here, we investigated traditional colony formation assays to determine if they also comprise cellular communication affecting the radiation response, using colony formation assays with varying numbers of cells, modulated beam irradiation and media transfer. Our findings showed that surviving fraction gradually increased with increasing number of irradiated cells. Specifically, for DU-145 human prostate cancer cells, surviving fraction increased 1.9-to-4.1-fold after 5-12 Gy irradiation; and for MM576 human melanoma cells, surviving fraction increased 1.9-fold after 5 Gy irradiation. Furthermore, increased surviving fraction was evident after modulated beam irradiation, where irradiated cells could communicate with nonirradiated cells. Media from dense cell culture also increased surviving fraction. The results suggest that traditional colony formation assays comprise unavoidable cellular communication affecting radiation outcome and the shape of the survival curve. We also propose that the increased in-field surviving fraction after modulated beam irradiation is due to the same effect.
(Less)
- author
- Adrian, Gabriel
LU
; Ceberg, Crister
LU
; Carneiro, Ana
LU
and Ekblad, Lars
LU
- organization
- publishing date
- 2018-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Journal Article
- in
- Radiation Research
- volume
- 189
- issue
- 1
- pages
- 9 pages
- publisher
- Radiation Research Society
- external identifiers
-
- pmid:29136392
- scopus:85042382522
- ISSN
- 0033-7587
- DOI
- 10.1667/RR14842.1
- project
- Signaling mediated effects of radiation therapy
- language
- English
- LU publication?
- yes
- id
- c27f7d1c-8629-464f-8d28-f83560cf9304
- date added to LUP
- 2018-01-12 14:12:44
- date last changed
- 2024-04-15 00:02:53
@article{c27f7d1c-8629-464f-8d28-f83560cf9304,
abstract = {{<p>It is well known that nonirradiated cells can exhibit radiation damage (bystander effect), and recent findings have shown that nonirradiated cells may help protect irradiated cells (rescue effect). These findings call into question the traditional view of radiation response: cells cannot be envisioned as isolated units. Here, we investigated traditional colony formation assays to determine if they also comprise cellular communication affecting the radiation response, using colony formation assays with varying numbers of cells, modulated beam irradiation and media transfer. Our findings showed that surviving fraction gradually increased with increasing number of irradiated cells. Specifically, for DU-145 human prostate cancer cells, surviving fraction increased 1.9-to-4.1-fold after 5-12 Gy irradiation; and for MM576 human melanoma cells, surviving fraction increased 1.9-fold after 5 Gy irradiation. Furthermore, increased surviving fraction was evident after modulated beam irradiation, where irradiated cells could communicate with nonirradiated cells. Media from dense cell culture also increased surviving fraction. The results suggest that traditional colony formation assays comprise unavoidable cellular communication affecting radiation outcome and the shape of the survival curve. We also propose that the increased in-field surviving fraction after modulated beam irradiation is due to the same effect.</p>}},
author = {{Adrian, Gabriel and Ceberg, Crister and Carneiro, Ana and Ekblad, Lars}},
issn = {{0033-7587}},
keywords = {{Journal Article}},
language = {{eng}},
number = {{1}},
pages = {{44--52}},
publisher = {{Radiation Research Society}},
series = {{Radiation Research}},
title = {{Rescue Effect Inherited in Colony Formation Assays Affects Radiation Response}},
url = {{http://dx.doi.org/10.1667/RR14842.1}},
doi = {{10.1667/RR14842.1}},
volume = {{189}},
year = {{2018}},
}