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Subtilisin inhibitors from legume seeds : A purification procedure

Tovar, Juscelino LU orcid ; Lorenzo, Pilar LU and Seidl, Dinah S. (1985) In Journal of the Science of Food and Agriculture 36(12). p.1285-1290
Abstract

Subtilisin inhibitors were prepared from ethanol‐acetone precipitated seed extracts of chick peas, jack beans and broad beans, heated to destroy endogenous proteases. Trypsin inhibitors were complexed with trypsin and separated from subtilisin inhibitors by molecular sieve chromatography. The eluates were again heated to avoid tryptic degradation. The procedure was easy to handle and gave satisfactory yields. In a final step two subtilisin isoinhibitors from chick peas were purified to electrophoretic homogeneity. They constitute 0.01% of the defatted dry weight.

Please use this url to cite or link to this publication:
author
; and
publishing date
type
Contribution to journal
publication status
published
keywords
broad beans, chick peas, jack beans, Proteinase inhibitors, purification procedure, subtilisin inhibitors
in
Journal of the Science of Food and Agriculture
volume
36
issue
12
pages
6 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:84985387035
ISSN
0022-5142
DOI
10.1002/jsfa.2740361213
language
English
LU publication?
no
id
c7e3bce4-766e-423d-8148-ac1c9b839a87
date added to LUP
2018-10-05 16:46:45
date last changed
2024-01-15 02:55:21
@article{c7e3bce4-766e-423d-8148-ac1c9b839a87,
  abstract     = {{<p>Subtilisin inhibitors were prepared from ethanol‐acetone precipitated seed extracts of chick peas, jack beans and broad beans, heated to destroy endogenous proteases. Trypsin inhibitors were complexed with trypsin and separated from subtilisin inhibitors by molecular sieve chromatography. The eluates were again heated to avoid tryptic degradation. The procedure was easy to handle and gave satisfactory yields. In a final step two subtilisin isoinhibitors from chick peas were purified to electrophoretic homogeneity. They constitute 0.01% of the defatted dry weight.</p>}},
  author       = {{Tovar, Juscelino and Lorenzo, Pilar and Seidl, Dinah S.}},
  issn         = {{0022-5142}},
  keywords     = {{broad beans; chick peas; jack beans; Proteinase inhibitors; purification procedure; subtilisin inhibitors}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{12}},
  pages        = {{1285--1290}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Journal of the Science of Food and Agriculture}},
  title        = {{Subtilisin inhibitors from legume seeds : A purification procedure}},
  url          = {{http://dx.doi.org/10.1002/jsfa.2740361213}},
  doi          = {{10.1002/jsfa.2740361213}},
  volume       = {{36}},
  year         = {{1985}},
}