The importance of a β-glucan receptor in the nonopsonic entry of nontypeable Haemophilus influenzae into human monocytic and epithelial cells
(2001) In Journal of Infectious Diseases 184(2). p.150-158- Abstract
Previous reports showed that nontypeable Haemophilus influenzae (NTHi) reside in macrophage-like cells in human adenoid tissue. This study investigated the ability of nonopsonized NTHi and encapsulated H. influenzae type b (Hib) to enter human monocytic and epithelial cells. The number of intracellular bacteria was determined by a viability assay and flow cytometry. To characterize the mechanisms responsible for the internalization of NTHi, different inhibitors of surface molecules, receptor turnover, and the cytoskeleton were used. Hib were found in monocytic cells at very low numbers (<100 bacteria/2 × 105 cells). In contrast, a great variation in intracellular numbers was detected between the different NTHi isolates... (More)
Previous reports showed that nontypeable Haemophilus influenzae (NTHi) reside in macrophage-like cells in human adenoid tissue. This study investigated the ability of nonopsonized NTHi and encapsulated H. influenzae type b (Hib) to enter human monocytic and epithelial cells. The number of intracellular bacteria was determined by a viability assay and flow cytometry. To characterize the mechanisms responsible for the internalization of NTHi, different inhibitors of surface molecules, receptor turnover, and the cytoskeleton were used. Hib were found in monocytic cells at very low numbers (<100 bacteria/2 × 105 cells). In contrast, a great variation in intracellular numbers was detected between the different NTHi isolates (range, 0.0007%-0.28% of the inoculum for monocytes and 0.053%-3.5% for epithelial cells). NTHi entered human monocytic and epithelial cells via a receptor-mediated endocytosis involving mainly a β-glucan receptor that could be blocked by laminarin.
(Less)
- author
- Ahrén, Irini Lazou LU ; Williams, David L. ; Rice, Peter J. ; Forsgren, Arne LU and Riesbeck, Kristian LU
- organization
- publishing date
- 2001-07-15
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Infectious Diseases
- volume
- 184
- issue
- 2
- pages
- 150 - 158
- publisher
- Oxford University Press
- external identifiers
-
- pmid:11424011
- scopus:0035879824
- ISSN
- 0022-1899
- DOI
- 10.1086/322016
- language
- English
- LU publication?
- yes
- id
- d064482a-1045-44a9-893b-2feb1bd16013
- date added to LUP
- 2019-06-07 15:09:57
- date last changed
- 2024-07-23 20:30:36
@article{d064482a-1045-44a9-893b-2feb1bd16013, abstract = {{<p>Previous reports showed that nontypeable Haemophilus influenzae (NTHi) reside in macrophage-like cells in human adenoid tissue. This study investigated the ability of nonopsonized NTHi and encapsulated H. influenzae type b (Hib) to enter human monocytic and epithelial cells. The number of intracellular bacteria was determined by a viability assay and flow cytometry. To characterize the mechanisms responsible for the internalization of NTHi, different inhibitors of surface molecules, receptor turnover, and the cytoskeleton were used. Hib were found in monocytic cells at very low numbers (<100 bacteria/2 × 10<sup>5</sup> cells). In contrast, a great variation in intracellular numbers was detected between the different NTHi isolates (range, 0.0007%-0.28% of the inoculum for monocytes and 0.053%-3.5% for epithelial cells). NTHi entered human monocytic and epithelial cells via a receptor-mediated endocytosis involving mainly a β-glucan receptor that could be blocked by laminarin.</p>}}, author = {{Ahrén, Irini Lazou and Williams, David L. and Rice, Peter J. and Forsgren, Arne and Riesbeck, Kristian}}, issn = {{0022-1899}}, language = {{eng}}, month = {{07}}, number = {{2}}, pages = {{150--158}}, publisher = {{Oxford University Press}}, series = {{Journal of Infectious Diseases}}, title = {{The importance of a β-glucan receptor in the nonopsonic entry of nontypeable Haemophilus influenzae into human monocytic and epithelial cells}}, url = {{http://dx.doi.org/10.1086/322016}}, doi = {{10.1086/322016}}, volume = {{184}}, year = {{2001}}, }