Acoustic Enrichment of Extracellular Vesicles from Biological Fluids
(2018) In Analytical Chemistry 90(13). p.8011-8019- Abstract
Extracellular vesicles (EVs) have emerged as a rich source of biomarkers providing diagnostic and prognostic information in diseases such as cancer. Large-scale investigations into the contents of EVs in clinical cohorts are warranted, but a major obstacle is the lack of a rapid, reproducible, efficient, and low-cost methodology to enrich EVs. Here, we demonstrate the applicability of an automated acoustic-based technique to enrich EVs, termed acoustic trapping. Using this technology, we have successfully enriched EVs from cell culture conditioned media and urine and blood plasma from healthy volunteers. The acoustically trapped samples contained EVs ranging from exosomes to microvesicles in size and contained detectable levels of... (More)
Extracellular vesicles (EVs) have emerged as a rich source of biomarkers providing diagnostic and prognostic information in diseases such as cancer. Large-scale investigations into the contents of EVs in clinical cohorts are warranted, but a major obstacle is the lack of a rapid, reproducible, efficient, and low-cost methodology to enrich EVs. Here, we demonstrate the applicability of an automated acoustic-based technique to enrich EVs, termed acoustic trapping. Using this technology, we have successfully enriched EVs from cell culture conditioned media and urine and blood plasma from healthy volunteers. The acoustically trapped samples contained EVs ranging from exosomes to microvesicles in size and contained detectable levels of intravesicular microRNAs. Importantly, this method showed high reproducibility and yielded sufficient quantities of vesicles for downstream analysis. The enrichment could be obtained from a sample volume of 300 μL or less, an equivalent to 30 min of enrichment time, depending on the sensitivity of downstream analysis. Taken together, acoustic trapping provides a rapid, automated, low-volume compatible, and robust method to enrich EVs from biofluids. Thus, it may serve as a novel tool for EV enrichment from large number of samples in a clinical setting with minimum sample preparation.
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- author
- Ku, Anson LU ; Lim, Hooi Ching LU ; Evander, Mikael LU ; Lilja, Hans LU ; Laurell, Thomas LU ; Scheding, Stefan LU and Ceder, Yvonne LU
- organization
-
- Acoustofluidics group (research group)
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
- Medical Molecular Biology (research group)
- Clinical Chemistry, Malmö (research group)
- Division of Translational Cancer Research
- Division of Molecular Hematology (DMH)
- StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
- Stem Cell Center
- Department of Biomedical Engineering
- NanoLund: Centre for Nanoscience
- EpiHealth: Epidemiology for Health
- publishing date
- 2018
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Acoustics, Cell Fractionation/instrumentation, Cell Line, Tumor, Exosomes/metabolism, Extracellular Vesicles/metabolism, Humans, MicroRNAs/genetics, Plasma/cytology, Urine/cytology
- in
- Analytical Chemistry
- volume
- 90
- issue
- 13
- pages
- 8011 - 8019
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- pmid:29806448
- scopus:85047778828
- ISSN
- 1520-6882
- DOI
- 10.1021/acs.analchem.8b00914
- language
- English
- LU publication?
- yes
- id
- d27a36ce-ba08-498e-a743-183f7874c1db
- date added to LUP
- 2019-05-22 08:59:44
- date last changed
- 2024-09-18 21:19:41
@article{d27a36ce-ba08-498e-a743-183f7874c1db, abstract = {{<p>Extracellular vesicles (EVs) have emerged as a rich source of biomarkers providing diagnostic and prognostic information in diseases such as cancer. Large-scale investigations into the contents of EVs in clinical cohorts are warranted, but a major obstacle is the lack of a rapid, reproducible, efficient, and low-cost methodology to enrich EVs. Here, we demonstrate the applicability of an automated acoustic-based technique to enrich EVs, termed acoustic trapping. Using this technology, we have successfully enriched EVs from cell culture conditioned media and urine and blood plasma from healthy volunteers. The acoustically trapped samples contained EVs ranging from exosomes to microvesicles in size and contained detectable levels of intravesicular microRNAs. Importantly, this method showed high reproducibility and yielded sufficient quantities of vesicles for downstream analysis. The enrichment could be obtained from a sample volume of 300 μL or less, an equivalent to 30 min of enrichment time, depending on the sensitivity of downstream analysis. Taken together, acoustic trapping provides a rapid, automated, low-volume compatible, and robust method to enrich EVs from biofluids. Thus, it may serve as a novel tool for EV enrichment from large number of samples in a clinical setting with minimum sample preparation.</p>}}, author = {{Ku, Anson and Lim, Hooi Ching and Evander, Mikael and Lilja, Hans and Laurell, Thomas and Scheding, Stefan and Ceder, Yvonne}}, issn = {{1520-6882}}, keywords = {{Acoustics; Cell Fractionation/instrumentation; Cell Line, Tumor; Exosomes/metabolism; Extracellular Vesicles/metabolism; Humans; MicroRNAs/genetics; Plasma/cytology; Urine/cytology}}, language = {{eng}}, number = {{13}}, pages = {{8011--8019}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Analytical Chemistry}}, title = {{Acoustic Enrichment of Extracellular Vesicles from Biological Fluids}}, url = {{http://dx.doi.org/10.1021/acs.analchem.8b00914}}, doi = {{10.1021/acs.analchem.8b00914}}, volume = {{90}}, year = {{2018}}, }