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Antimicrobial Peptide Coating of TiO2 Nanoparticles for Boosted Antimicrobial Effects

Caselli, Lucrezia LU ; Traini, Tanja ; Micciulla, Samantha ; Sebastiani, Federica LU ; Köhler, Sebastian LU ; Nielsen, Emilie Marie ; Diedrichsen, Ragna Guldsmed ; Skoda, Maximilian W.A. and Malmsten, Martin LU (2024) In Advanced Functional Materials
Abstract

This study explores the coating of photocatalytic nanoparticles with antimicrobial peptides (AMPs) for boosted antimicrobial effects, and how such effects depend on AMP properties. For this, TiO2 nanoparticles are coated with the AMP KYE21 or its hydrophobically enhanced variant WWWKYE21. Mirroring effects of free peptides, coated nanoparticles displayed higher binding and UV-induced degradation for bacteria-like than for mammalian-like membranes. In addition, they degraded bacterial lipopolysaccharides (LPS). WWWKYE21-coated nanoparticles displayed higher binding to LPS and bacteria-like membranes and photocatalytic degradation, although saturation effects are found at high nanoparticle binding. Neutron reflectometry showed... (More)

This study explores the coating of photocatalytic nanoparticles with antimicrobial peptides (AMPs) for boosted antimicrobial effects, and how such effects depend on AMP properties. For this, TiO2 nanoparticles are coated with the AMP KYE21 or its hydrophobically enhanced variant WWWKYE21. Mirroring effects of free peptides, coated nanoparticles displayed higher binding and UV-induced degradation for bacteria-like than for mammalian-like membranes. In addition, they degraded bacterial lipopolysaccharides (LPS). WWWKYE21-coated nanoparticles displayed higher binding to LPS and bacteria-like membranes and photocatalytic degradation, although saturation effects are found at high nanoparticle binding. Neutron reflectometry showed that binding of peptide-coated nanoparticles to bacteria-like membranes resulted in partial lipid removal in the absence of UV, but that UV illumination caused additional degradation, featuring increases in the hydration of headgroup and acyl chain regions. For LPS, UV induced removal of its outer O-antigen region. Analogous to findings in model systems, antimicrobial effects of peptide-coated nanoparticles against Escherichia coli bacteria on illumination are pronounced, while toxicity against human monocytes remained low. Altogether, results show that AMP coating boosts the antimicrobial effects of photocatalytic nanoparticles without causing cell toxicity. From a broader perspective, the study points to the potential of nanoarchitectonic combination of component properties for the design of composite NP properties.

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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
in press
subject
keywords
antimicrobial peptide, membrane, nanoparticle, photocatalysis, TiO, tryptophan
in
Advanced Functional Materials
publisher
Wiley-Blackwell
external identifiers
  • scopus:85196430191
ISSN
1616-301X
DOI
10.1002/adfm.202405047
language
English
LU publication?
yes
id
e0773bc2-2c6d-4b55-9238-6b8795e71273
date added to LUP
2024-09-11 14:19:19
date last changed
2024-09-11 14:19:19
@article{e0773bc2-2c6d-4b55-9238-6b8795e71273,
  abstract     = {{<p>This study explores the coating of photocatalytic nanoparticles with antimicrobial peptides (AMPs) for boosted antimicrobial effects, and how such effects depend on AMP properties. For this, TiO<sub>2</sub> nanoparticles are coated with the AMP KYE21 or its hydrophobically enhanced variant WWWKYE21. Mirroring effects of free peptides, coated nanoparticles displayed higher binding and UV-induced degradation for bacteria-like than for mammalian-like membranes. In addition, they degraded bacterial lipopolysaccharides (LPS). WWWKYE21-coated nanoparticles displayed higher binding to LPS and bacteria-like membranes and photocatalytic degradation, although saturation effects are found at high nanoparticle binding. Neutron reflectometry showed that binding of peptide-coated nanoparticles to bacteria-like membranes resulted in partial lipid removal in the absence of UV, but that UV illumination caused additional degradation, featuring increases in the hydration of headgroup and acyl chain regions. For LPS, UV induced removal of its outer O-antigen region. Analogous to findings in model systems, antimicrobial effects of peptide-coated nanoparticles against Escherichia coli bacteria on illumination are pronounced, while toxicity against human monocytes remained low. Altogether, results show that AMP coating boosts the antimicrobial effects of photocatalytic nanoparticles without causing cell toxicity. From a broader perspective, the study points to the potential of nanoarchitectonic combination of component properties for the design of composite NP properties.</p>}},
  author       = {{Caselli, Lucrezia and Traini, Tanja and Micciulla, Samantha and Sebastiani, Federica and Köhler, Sebastian and Nielsen, Emilie Marie and Diedrichsen, Ragna Guldsmed and Skoda, Maximilian W.A. and Malmsten, Martin}},
  issn         = {{1616-301X}},
  keywords     = {{antimicrobial peptide; membrane; nanoparticle; photocatalysis; TiO; tryptophan}},
  language     = {{eng}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Advanced Functional Materials}},
  title        = {{Antimicrobial Peptide Coating of TiO<sub>2</sub> Nanoparticles for Boosted Antimicrobial Effects}},
  url          = {{http://dx.doi.org/10.1002/adfm.202405047}},
  doi          = {{10.1002/adfm.202405047}},
  year         = {{2024}},
}