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Purification of IgD from human serum - A novel application of recombinant M. catarrhalis IgD-binding protein (MID).

Samuelsson, Martin LU ; Forsgren, Arne LU and Riesbeck, Kristian LU (2006) In Journal of Immunological Methods 317. p.31-37
Abstract
Moraxella catarrhalis IgD-binding protein (MID) is a multimeric outer membrane protein belonging to the family of autotransporters. The IgD-binding domain of MID is located between amino acids MID 962-1200 and binds to amino acids 198-224 of the IgD C(H)1 region. In the present study, we describe a method to purify IgD from serum with high levels of IgD using a two-step affinity chromatography process. The first step involves depletion of MID-specific antibodies of all classes from serum using the non-IgD-binding fragment MID1000-1200. This step is followed by selective capture of IgD with MID962-1200. Furthermore, we demonstrate that the eluted IgD is pure, intact and functional for use in downstream applications. Our approach reduces the... (More)
Moraxella catarrhalis IgD-binding protein (MID) is a multimeric outer membrane protein belonging to the family of autotransporters. The IgD-binding domain of MID is located between amino acids MID 962-1200 and binds to amino acids 198-224 of the IgD C(H)1 region. In the present study, we describe a method to purify IgD from serum with high levels of IgD using a two-step affinity chromatography process. The first step involves depletion of MID-specific antibodies of all classes from serum using the non-IgD-binding fragment MID1000-1200. This step is followed by selective capture of IgD with MID962-1200. Furthermore, we demonstrate that the eluted IgD is pure, intact and functional for use in downstream applications. Our approach reduces the non-specificity commonly associated with lectin-based IgD purification regimes that rely on glycosylation of the IgD molecule. (c) 2006 Elsevier B.V. All rights reserved. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
purification, immunoglobulin D, Moraxella catarrhalis, MID
in
Journal of Immunological Methods
volume
317
pages
31 - 37
publisher
Elsevier
external identifiers
  • wos:000243148700004
  • scopus:33751345343
ISSN
1872-7905
DOI
10.1016/j.jim.2006.09.007
language
English
LU publication?
yes
id
00f587aa-fe85-4f59-8a12-ce406bae0750 (old id 162214)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17056056&dopt=Abstract
date added to LUP
2007-07-26 16:24:19
date last changed
2017-08-27 05:24:27
@article{00f587aa-fe85-4f59-8a12-ce406bae0750,
  abstract     = {Moraxella catarrhalis IgD-binding protein (MID) is a multimeric outer membrane protein belonging to the family of autotransporters. The IgD-binding domain of MID is located between amino acids MID 962-1200 and binds to amino acids 198-224 of the IgD C(H)1 region. In the present study, we describe a method to purify IgD from serum with high levels of IgD using a two-step affinity chromatography process. The first step involves depletion of MID-specific antibodies of all classes from serum using the non-IgD-binding fragment MID1000-1200. This step is followed by selective capture of IgD with MID962-1200. Furthermore, we demonstrate that the eluted IgD is pure, intact and functional for use in downstream applications. Our approach reduces the non-specificity commonly associated with lectin-based IgD purification regimes that rely on glycosylation of the IgD molecule. (c) 2006 Elsevier B.V. All rights reserved.},
  author       = {Samuelsson, Martin and Forsgren, Arne and Riesbeck, Kristian},
  issn         = {1872-7905},
  keyword      = {purification,immunoglobulin D,Moraxella catarrhalis,MID},
  language     = {eng},
  pages        = {31--37},
  publisher    = {Elsevier},
  series       = {Journal of Immunological Methods},
  title        = {Purification of IgD from human serum - A novel application of recombinant M. catarrhalis IgD-binding protein (MID).},
  url          = {http://dx.doi.org/10.1016/j.jim.2006.09.007},
  volume       = {317},
  year         = {2006},
}