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Purification of IgD from human serum - A novel application of recombinant M. catarrhalis IgD-binding protein (MID).

Samuelsson, Martin LU ; Forsgren, Arne LU and Riesbeck, Kristian LU orcid (2006) In Journal of Immunological Methods 317. p.31-37
Abstract
Moraxella catarrhalis IgD-binding protein (MID) is a multimeric outer membrane protein belonging to the family of autotransporters. The IgD-binding domain of MID is located between amino acids MID 962-1200 and binds to amino acids 198-224 of the IgD C(H)1 region. In the present study, we describe a method to purify IgD from serum with high levels of IgD using a two-step affinity chromatography process. The first step involves depletion of MID-specific antibodies of all classes from serum using the non-IgD-binding fragment MID1000-1200. This step is followed by selective capture of IgD with MID962-1200. Furthermore, we demonstrate that the eluted IgD is pure, intact and functional for use in downstream applications. Our approach reduces the... (More)
Moraxella catarrhalis IgD-binding protein (MID) is a multimeric outer membrane protein belonging to the family of autotransporters. The IgD-binding domain of MID is located between amino acids MID 962-1200 and binds to amino acids 198-224 of the IgD C(H)1 region. In the present study, we describe a method to purify IgD from serum with high levels of IgD using a two-step affinity chromatography process. The first step involves depletion of MID-specific antibodies of all classes from serum using the non-IgD-binding fragment MID1000-1200. This step is followed by selective capture of IgD with MID962-1200. Furthermore, we demonstrate that the eluted IgD is pure, intact and functional for use in downstream applications. Our approach reduces the non-specificity commonly associated with lectin-based IgD purification regimes that rely on glycosylation of the IgD molecule. (c) 2006 Elsevier B.V. All rights reserved. (Less)
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author
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type
Contribution to journal
publication status
published
subject
keywords
purification, immunoglobulin D, Moraxella catarrhalis, MID
in
Journal of Immunological Methods
volume
317
pages
31 - 37
publisher
Elsevier
external identifiers
  • wos:000243148700004
  • scopus:33751345343
  • pmid:17056056
ISSN
1872-7905
DOI
10.1016/j.jim.2006.09.007
language
English
LU publication?
yes
id
00f587aa-fe85-4f59-8a12-ce406bae0750 (old id 162214)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17056056&dopt=Abstract
date added to LUP
2016-04-01 16:17:08
date last changed
2022-01-28 18:39:23
@article{00f587aa-fe85-4f59-8a12-ce406bae0750,
  abstract     = {{Moraxella catarrhalis IgD-binding protein (MID) is a multimeric outer membrane protein belonging to the family of autotransporters. The IgD-binding domain of MID is located between amino acids MID 962-1200 and binds to amino acids 198-224 of the IgD C(H)1 region. In the present study, we describe a method to purify IgD from serum with high levels of IgD using a two-step affinity chromatography process. The first step involves depletion of MID-specific antibodies of all classes from serum using the non-IgD-binding fragment MID1000-1200. This step is followed by selective capture of IgD with MID962-1200. Furthermore, we demonstrate that the eluted IgD is pure, intact and functional for use in downstream applications. Our approach reduces the non-specificity commonly associated with lectin-based IgD purification regimes that rely on glycosylation of the IgD molecule. (c) 2006 Elsevier B.V. All rights reserved.}},
  author       = {{Samuelsson, Martin and Forsgren, Arne and Riesbeck, Kristian}},
  issn         = {{1872-7905}},
  keywords     = {{purification; immunoglobulin D; Moraxella catarrhalis; MID}},
  language     = {{eng}},
  pages        = {{31--37}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Immunological Methods}},
  title        = {{Purification of IgD from human serum - A novel application of recombinant M. catarrhalis IgD-binding protein (MID).}},
  url          = {{http://dx.doi.org/10.1016/j.jim.2006.09.007}},
  doi          = {{10.1016/j.jim.2006.09.007}},
  volume       = {{317}},
  year         = {{2006}},
}