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Changes in Intestinal Permeability Ex Vivo and Immune Cell Activation by Three Commonly Used Emulsifiers

Oscarsson, Elin LU ; Lindberg, Tim LU ; Zeller, Kathrin S. LU orcid ; Lindstedt, Malin LU orcid ; Agardh, Daniel LU ; Håkansson, Åsa LU and Östbring, Karolina LU orcid (2020) In Molecules (Basel, Switzerland) 25(24).
Abstract

Food additives such as emulsifiers are used in increasing quantities in the food industry. The aim of this study was to compare three different emulsifiers (polysorbate 80 (P80), carboxymethyl cellulose (CMC), and β-lactoglobulin (β-lac) with regards to their effect on the stimulation of immune cells and intestinal permeability. The immune stimulatory effects were studied in the myeloid cell line MUTZ-3-cells, while the change in intestinal permeability was studied in the Caco-2 cell line and ex vivo in the Ussing chamber system using small intestinal fragments from rats. The tested concentrations of the emulsifiers ranged from 0.02% up to 1%, which are concentrations commonly used in the food industry. The results showed that P80... (More)

Food additives such as emulsifiers are used in increasing quantities in the food industry. The aim of this study was to compare three different emulsifiers (polysorbate 80 (P80), carboxymethyl cellulose (CMC), and β-lactoglobulin (β-lac) with regards to their effect on the stimulation of immune cells and intestinal permeability. The immune stimulatory effects were studied in the myeloid cell line MUTZ-3-cells, while the change in intestinal permeability was studied in the Caco-2 cell line and ex vivo in the Ussing chamber system using small intestinal fragments from rats. The tested concentrations of the emulsifiers ranged from 0.02% up to 1%, which are concentrations commonly used in the food industry. The results showed that P80 affected both the myeloid cells and the intestinal permeability more than CMC (p < 0.05) and β-lac (p < 0.05) at the highest concentration. CMC was found to neither affect the permeability in the intestine nor the MUTZ-3 cells, while β-lac changed the permeability in the total part of the small intestine in rats. These findings indicate that P80 might be more cytotoxic compared to the other two emulsifiers.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
beta-lactoglobulin, caco-2, carboxymethyl cellulose, dendritic cell activation, food emulsifiers, intestinal permeability, polysorbate 80, TEER, ussing chambers
in
Molecules (Basel, Switzerland)
volume
25
issue
24
article number
5943
publisher
MDPI AG
external identifiers
  • pmid:33333981
  • scopus:85098676685
ISSN
1420-3049
DOI
10.3390/molecules25245943
language
English
LU publication?
yes
id
0132a939-5198-480c-8fbe-5e548bd8d024
date added to LUP
2021-01-15 07:46:34
date last changed
2024-12-13 02:20:38
@article{0132a939-5198-480c-8fbe-5e548bd8d024,
  abstract     = {{<p>Food additives such as emulsifiers are used in increasing quantities in the food industry. The aim of this study was to compare three different emulsifiers (polysorbate 80 (P80), carboxymethyl cellulose (CMC), and β-lactoglobulin (β-lac) with regards to their effect on the stimulation of immune cells and intestinal permeability. The immune stimulatory effects were studied in the myeloid cell line MUTZ-3-cells, while the change in intestinal permeability was studied in the Caco-2 cell line and ex vivo in the Ussing chamber system using small intestinal fragments from rats. The tested concentrations of the emulsifiers ranged from 0.02% up to 1%, which are concentrations commonly used in the food industry. The results showed that P80 affected both the myeloid cells and the intestinal permeability more than CMC (p &lt; 0.05) and β-lac (p &lt; 0.05) at the highest concentration. CMC was found to neither affect the permeability in the intestine nor the MUTZ-3 cells, while β-lac changed the permeability in the total part of the small intestine in rats. These findings indicate that P80 might be more cytotoxic compared to the other two emulsifiers.</p>}},
  author       = {{Oscarsson, Elin and Lindberg, Tim and Zeller, Kathrin S. and Lindstedt, Malin and Agardh, Daniel and Håkansson, Åsa and Östbring, Karolina}},
  issn         = {{1420-3049}},
  keywords     = {{beta-lactoglobulin; caco-2; carboxymethyl cellulose; dendritic cell activation; food emulsifiers; intestinal permeability; polysorbate 80; TEER; ussing chambers}},
  language     = {{eng}},
  number       = {{24}},
  publisher    = {{MDPI AG}},
  series       = {{Molecules (Basel, Switzerland)}},
  title        = {{Changes in Intestinal Permeability Ex Vivo and Immune Cell Activation by Three Commonly Used Emulsifiers}},
  url          = {{http://dx.doi.org/10.3390/molecules25245943}},
  doi          = {{10.3390/molecules25245943}},
  volume       = {{25}},
  year         = {{2020}},
}