Quantification of heat shock proteins in the posterior interosseous nerve among subjects with type 1 and type 2 diabetes compared to healthy controls
(2023) In Frontiers in Neuroscience 17.- Abstract
- Introduction: Diabetic peripheral neuropathy (DPN) is a common complication of both type 1 (T1D) and type 2 diabetes (T2D). No cure for DPN is available, but several potential targets have been proposed for treatment. Heat shock proteins (HSPs) are known to respond to both hyper- and hypoglycemia. DPN can be diagnosed using electrophysiology and studied using peripheral nerve biopsies.
Aim: This study aimed to analyze the presence and patterns of HSPs in peripheral nerve biopsies from subjects with T1D, T2D, and healthy controls.
Methods: Posterior interosseous nerves (PIN) from a total of 56 subjects with T1D (n = 9), with T2D (n = 24), and without diabetes (i.e., healthy controls, n = 23) were harvested under local... (More) - Introduction: Diabetic peripheral neuropathy (DPN) is a common complication of both type 1 (T1D) and type 2 diabetes (T2D). No cure for DPN is available, but several potential targets have been proposed for treatment. Heat shock proteins (HSPs) are known to respond to both hyper- and hypoglycemia. DPN can be diagnosed using electrophysiology and studied using peripheral nerve biopsies.
Aim: This study aimed to analyze the presence and patterns of HSPs in peripheral nerve biopsies from subjects with T1D, T2D, and healthy controls.
Methods: Posterior interosseous nerves (PIN) from a total of 56 subjects with T1D (n = 9), with T2D (n = 24), and without diabetes (i.e., healthy controls, n = 23) were harvested under local anesthesia and prepared for quantitative mass spectrometry analysis. Protein intensities were associated with electrophysiology data of the ulnar nerve and morphometry of the same PIN, and differences in protein intensities between groups were analyzed.
Results: In total, 32 different HSPs were identified and quantified in the nerve specimens. No statistically significant differences were observed regarding protein intensities between groups. Furthermore, protein intensities did not correlate with amplitude or conduction velocity in the ulnar nerve or with the myelinated nerve fiber density of PIN.
Conclusion: Quantitative proteomics can be used to study HSPs in nerve biopsies, but no clear differences in protein quantities were observed between groups in this cohort. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/0247f87d-ad50-46aa-b296-c1c886326df3
- author
- Ising, Erik LU ; Åhrman, Emma LU ; Thomsen, Niels O. B. LU ; Åkesson, Anna ; Malmström, Johan LU and Dahlin, Lars B. LU
- organization
-
- Paediatric Endocrinology (research group)
- EXODIAB: Excellence of Diabetes Research in Sweden
- Infection Medicine Proteomics (research group)
- Hand Surgery, Malmö (research group)
- LTH Profile Area: Engineering Health
- BioMS (research group)
- Mass Spectrometry
- epIgG (research group)
- SEBRA Sepsis and Bacterial Resistance Alliance (research group)
- Infection Medicine (BMC)
- EpiHealth: Epidemiology for Health
- WCMM-Wallenberg Centre for Molecular Medicine
- Department of Translational Medicine
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Frontiers in Neuroscience
- volume
- 17
- article number
- 1227557
- publisher
- Frontiers Media S. A.
- external identifiers
-
- scopus:85168486913
- pmid:37614345
- ISSN
- 1662-4548
- DOI
- 10.3389/fnins.2023.1227557
- language
- English
- LU publication?
- yes
- id
- 0247f87d-ad50-46aa-b296-c1c886326df3
- date added to LUP
- 2023-08-22 07:46:14
- date last changed
- 2023-11-22 03:00:20
@article{0247f87d-ad50-46aa-b296-c1c886326df3, abstract = {{Introduction: Diabetic peripheral neuropathy (DPN) is a common complication of both type 1 (T1D) and type 2 diabetes (T2D). No cure for DPN is available, but several potential targets have been proposed for treatment. Heat shock proteins (HSPs) are known to respond to both hyper- and hypoglycemia. DPN can be diagnosed using electrophysiology and studied using peripheral nerve biopsies.<br/><br/>Aim: This study aimed to analyze the presence and patterns of HSPs in peripheral nerve biopsies from subjects with T1D, T2D, and healthy controls.<br/><br/>Methods: Posterior interosseous nerves (PIN) from a total of 56 subjects with T1D (n = 9), with T2D (n = 24), and without diabetes (i.e., healthy controls, n = 23) were harvested under local anesthesia and prepared for quantitative mass spectrometry analysis. Protein intensities were associated with electrophysiology data of the ulnar nerve and morphometry of the same PIN, and differences in protein intensities between groups were analyzed.<br/><br/>Results: In total, 32 different HSPs were identified and quantified in the nerve specimens. No statistically significant differences were observed regarding protein intensities between groups. Furthermore, protein intensities did not correlate with amplitude or conduction velocity in the ulnar nerve or with the myelinated nerve fiber density of PIN.<br/><br/>Conclusion: Quantitative proteomics can be used to study HSPs in nerve biopsies, but no clear differences in protein quantities were observed between groups in this cohort.}}, author = {{Ising, Erik and Åhrman, Emma and Thomsen, Niels O. B. and Åkesson, Anna and Malmström, Johan and Dahlin, Lars B.}}, issn = {{1662-4548}}, language = {{eng}}, publisher = {{Frontiers Media S. A.}}, series = {{Frontiers in Neuroscience}}, title = {{Quantification of heat shock proteins in the posterior interosseous nerve among subjects with type 1 and type 2 diabetes compared to healthy controls}}, url = {{http://dx.doi.org/10.3389/fnins.2023.1227557}}, doi = {{10.3389/fnins.2023.1227557}}, volume = {{17}}, year = {{2023}}, }