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Individual differences of bacterial degradation of starch in the upper gastrointestinal tract evaluated in a modified in vitro model

Oscarsson, E LU ; Wahlgren, M LU orcid ; Agardh, D LU and Håkansson, Å LU orcid (2018) In Advances in Applied and Pharmaceutical Sciences Journal 2(2). p.31-38
Abstract
Avoiding absorption in the upper gastrointestinal tract is one of the challenges
considering targeted colon delivery but not much attention has been paid to
microbial activity of vector material in saliva and small intestine. The aim of the
present study was to develop an in vitro prototype model for evaluating
digestion of encapsulation material by oral bacterial species based on readily
obtainable saliva samples from 20 healthy volunteers with different nationality.
The relation between bacterial composition and starch digestion was analyzed as
well as individual differences in microbial composition. A difference in starch
digestion between individuals dependent on continent belongings was... (More)
Avoiding absorption in the upper gastrointestinal tract is one of the challenges
considering targeted colon delivery but not much attention has been paid to
microbial activity of vector material in saliva and small intestine. The aim of the
present study was to develop an in vitro prototype model for evaluating
digestion of encapsulation material by oral bacterial species based on readily
obtainable saliva samples from 20 healthy volunteers with different nationality.
The relation between bacterial composition and starch digestion was analyzed as
well as individual differences in microbial composition. A difference in starch
digestion between individuals dependent on continent belongings was detected.
European samples had higher degradation than samples from Asia and America
(p= 0.003). The digestion was independent on amylase impact, total amount of
bacteria and diversity, indicating bacterial species as being important. The T-RFs
found to correlate to high concentration of glucose were putatively designated to
the phyla Bacteroidetes, Fusobacteria and to an unknown species. In
conclusion, a difference in starch digestion based on microbial fermentation in
the upper gastrointestinal tract could be detected using this pilot in vitro model
but which species or groups of bacteria that are significant for the digestion
needs to be further evaluated. (Less)
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Advances in Applied and Pharmaceutical Sciences Journal
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2
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8 pages
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yes
id
038a5321-69a7-4349-aa89-651bbeccae49
date added to LUP
2018-11-15 23:13:50
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2025-09-25 15:00:18
@article{038a5321-69a7-4349-aa89-651bbeccae49,
  abstract     = {{Avoiding absorption in the upper gastrointestinal tract is one of the challenges<br/>considering targeted colon delivery but not much attention has been paid to<br/>microbial activity of vector material in saliva and small intestine. The aim of the<br/>present study was to develop an in vitro prototype model for evaluating<br/>digestion of encapsulation material by oral bacterial species based on readily<br/>obtainable saliva samples from 20 healthy volunteers with different nationality.<br/>The relation between bacterial composition and starch digestion was analyzed as<br/>well as individual differences in microbial composition. A difference in starch<br/>digestion between individuals dependent on continent belongings was detected.<br/>European samples had higher degradation than samples from Asia and America<br/>(p= 0.003). The digestion was independent on amylase impact, total amount of<br/>bacteria and diversity, indicating bacterial species as being important. The T-RFs<br/>found to correlate to high concentration of glucose were putatively designated to<br/>the phyla Bacteroidetes, Fusobacteria and to an unknown species. In<br/>conclusion, a difference in starch digestion based on microbial fermentation in<br/>the upper gastrointestinal tract could be detected using this pilot in vitro model<br/>but which species or groups of bacteria that are significant for the digestion<br/>needs to be further evaluated.}},
  author       = {{Oscarsson, E and Wahlgren, M and Agardh, D and Håkansson, Å}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{31--38}},
  series       = {{Advances in Applied and Pharmaceutical Sciences Journal}},
  title        = {{Individual differences of bacterial degradation of starch in the upper gastrointestinal tract evaluated in a modified <i>in vitro </i>model}},
  volume       = {{2}},
  year         = {{2018}},
}