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Adsorption during heat treatment related to the thermal unfolding/aggregation of β-lactoglobulins A and B

Elofsson, Ulla M. ; Paulsson, Marie A. LU orcid ; Sellers, Peter LU and Arnebrant, Thomas (1996) In Journal of Colloid and Interface Science 183(2). p.408-415
Abstract
Adsorption onto chromium surfaces during heat treatment (65–68°C) of
β-lactoglobulin A and B in phosphate buffer, pH 6.88, was investigated
byin situellipsometry. Thermal unfolding andin situheat-induced
aggregation under the same conditions were studied by differential
scanning calorimetry and dynamic light scattering, respectively. A lag
phase was observed for buildup of the thickness of the adsorbed layer of
β-lactoglobulin A and B at 68°C. The lag period was found to increase
with decreasing temperature. The difference in adsorption rate between
the variants was found to be in agreement with the aggregation
difference in solution, that is, the A variant aggregated more... (More)
Adsorption onto chromium surfaces during heat treatment (65–68°C) of
β-lactoglobulin A and B in phosphate buffer, pH 6.88, was investigated
byin situellipsometry. Thermal unfolding andin situheat-induced
aggregation under the same conditions were studied by differential
scanning calorimetry and dynamic light scattering, respectively. A lag
phase was observed for buildup of the thickness of the adsorbed layer of
β-lactoglobulin A and B at 68°C. The lag period was found to increase
with decreasing temperature. The difference in adsorption rate between
the variants was found to be in agreement with the aggregation
difference in solution, that is, the A variant aggregated more rapidly
after a somewhat slower beginning. These results could be related to a
difference in the thermal unfolding behavior. By applying a non-two
state model the second of two transitions was suggested to involve the
activation of the free sulfydryl group. The results indicated that
fouling proceeds via attachment of aggregates with an activated free
sulfydryl group through an interchange reaction with a disulfide bond in
the adsorbed protein layer. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Colloid and Interface Science
volume
183
issue
2
pages
8 pages
publisher
Academic Press
external identifiers
  • scopus:0030579375
ISSN
0021-9797
DOI
10.1006/jcis.1996.0563
language
English
LU publication?
yes
id
097ab264-25ca-4759-9b00-2caecb91e133
date added to LUP
2025-09-19 15:24:42
date last changed
2025-10-14 12:42:10
@article{097ab264-25ca-4759-9b00-2caecb91e133,
  abstract     = {{Adsorption onto chromium surfaces during heat treatment (65–68°C) of <br>
β-lactoglobulin A and B in phosphate buffer, pH 6.88, was investigated <br>
by<em>in situ</em>ellipsometry. Thermal unfolding and<em>in situ</em>heat-induced<br>
 aggregation under the same conditions were studied by differential <br>
scanning calorimetry and dynamic light scattering, respectively. A lag <br>
phase was observed for buildup of the thickness of the adsorbed layer of<br>
 β-lactoglobulin A and B at 68°C. The lag period was found to increase <br>
with decreasing temperature. The difference in adsorption rate between <br>
the variants was found to be in agreement with the aggregation <br>
difference in solution, that is, the A variant aggregated more rapidly <br>
after a somewhat slower beginning. These results could be related to a <br>
difference in the thermal unfolding behavior. By applying a non-two <br>
state model the second of two transitions was suggested to involve the <br>
activation of the free sulfydryl group. The results indicated that <br>
fouling proceeds via attachment of aggregates with an activated free <br>
sulfydryl group through an interchange reaction with a disulfide bond in<br>
 the adsorbed protein layer.}},
  author       = {{Elofsson, Ulla M. and Paulsson, Marie A. and Sellers, Peter and Arnebrant, Thomas}},
  issn         = {{0021-9797}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{408--415}},
  publisher    = {{Academic Press}},
  series       = {{Journal of Colloid and Interface Science}},
  title        = {{Adsorption during heat treatment related to the thermal unfolding/aggregation of β-lactoglobulins A and B}},
  url          = {{http://dx.doi.org/10.1006/jcis.1996.0563}},
  doi          = {{10.1006/jcis.1996.0563}},
  volume       = {{183}},
  year         = {{1996}},
}