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Evaluation of multiple oxidation products for monitoring effects of antioxidants in Fenton oxidation of 2 '-deoxyguanosine

Grey, Carl LU and Adlercreutz, Patrick LU (2006) In Journal of Agricultural and Food Chemistry 54(6). p.2350-2358
Abstract
The objective of this study was to investigate the influence of the two antioxidants, ascorbic acid and (+)catechin, on the oxidation of 2'-deoxyguanosine (dG), using an iron-mediated Fenton reaction. The oxidation products 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8,5'-cyclo-2'-deoxyguanosine, together with the secondary oxidation products guanidinohydantoin and dehydroguanidinohydantoin, were identified and quantified through the use of an LC-MS/MS system. The results obtained showed that catechin inhibited the oxidation better than ascorbic acid did, indicating that the chelating ability of catechin rather than the radical scavenging mechanism alone is vital for the observed antioxidative efficiency. The correlation between the... (More)
The objective of this study was to investigate the influence of the two antioxidants, ascorbic acid and (+)catechin, on the oxidation of 2'-deoxyguanosine (dG), using an iron-mediated Fenton reaction. The oxidation products 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8,5'-cyclo-2'-deoxyguanosine, together with the secondary oxidation products guanidinohydantoin and dehydroguanidinohydantoin, were identified and quantified through the use of an LC-MS/MS system. The results obtained showed that catechin inhibited the oxidation better than ascorbic acid did, indicating that the chelating ability of catechin rather than the radical scavenging mechanism alone is vital for the observed antioxidative efficiency. The correlation between the different oxidation products was found to be quite low, primarily because of the instability of 8-oxodG, making it prone to further oxidation. This led to apparent anti- and pro-oxidative results being obtained, emphasizing the potential problems in evaluating oxidative stress, by use of a single marker. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ascorbic acid, Fenton reaction, oxidation, 2 '-deoxyguanosine, catechin
in
Journal of Agricultural and Food Chemistry
volume
54
issue
6
pages
2350 - 2358
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000236227100057
  • pmid:16536618
  • scopus:33645792170
ISSN
0021-8561
DOI
10.1021/jf052202q
language
English
LU publication?
yes
id
0a0114f5-b7e0-4ccc-a508-dc26a47e2988 (old id 415179)
date added to LUP
2007-10-03 11:01:10
date last changed
2019-08-23 02:23:08
@article{0a0114f5-b7e0-4ccc-a508-dc26a47e2988,
  abstract     = {The objective of this study was to investigate the influence of the two antioxidants, ascorbic acid and (+)catechin, on the oxidation of 2'-deoxyguanosine (dG), using an iron-mediated Fenton reaction. The oxidation products 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8,5'-cyclo-2'-deoxyguanosine, together with the secondary oxidation products guanidinohydantoin and dehydroguanidinohydantoin, were identified and quantified through the use of an LC-MS/MS system. The results obtained showed that catechin inhibited the oxidation better than ascorbic acid did, indicating that the chelating ability of catechin rather than the radical scavenging mechanism alone is vital for the observed antioxidative efficiency. The correlation between the different oxidation products was found to be quite low, primarily because of the instability of 8-oxodG, making it prone to further oxidation. This led to apparent anti- and pro-oxidative results being obtained, emphasizing the potential problems in evaluating oxidative stress, by use of a single marker.},
  author       = {Grey, Carl and Adlercreutz, Patrick},
  issn         = {0021-8561},
  keyword      = {ascorbic acid,Fenton reaction,oxidation,2 '-deoxyguanosine,catechin},
  language     = {eng},
  number       = {6},
  pages        = {2350--2358},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Journal of Agricultural and Food Chemistry},
  title        = {Evaluation of multiple oxidation products for monitoring effects of antioxidants in Fenton oxidation of 2 '-deoxyguanosine},
  url          = {http://dx.doi.org/10.1021/jf052202q},
  volume       = {54},
  year         = {2006},
}