The human bone marrow harbors a CD45− CD11B+ cell progenitor permitting rapid microglia-like cell derivative approaches
(2021) In Stem cells translational medicine 10(4). p.582-597- Abstract
Microglia, the immune sentinel of the central nervous system (CNS), are generated from yolk sac erythromyeloid progenitors that populate the developing CNS. Interestingly, a specific type of bone marrow-derived monocyte is able to express a yolk sac microglial signature and populate CNS in disease. Here we have examined human bone marrow (hBM) in an attempt to identify novel cell sources for generating microglia-like cells to use in cell-based therapies and in vitro modeling. We demonstrate that hBM stroma harbors a progenitor cell that we name stromal microglial progenitor (STR-MP). STR-MP single-cell gene analysis revealed the expression of the consensus genetic microglial signature and microglial-specific genes present in development... (More)
Microglia, the immune sentinel of the central nervous system (CNS), are generated from yolk sac erythromyeloid progenitors that populate the developing CNS. Interestingly, a specific type of bone marrow-derived monocyte is able to express a yolk sac microglial signature and populate CNS in disease. Here we have examined human bone marrow (hBM) in an attempt to identify novel cell sources for generating microglia-like cells to use in cell-based therapies and in vitro modeling. We demonstrate that hBM stroma harbors a progenitor cell that we name stromal microglial progenitor (STR-MP). STR-MP single-cell gene analysis revealed the expression of the consensus genetic microglial signature and microglial-specific genes present in development and CNS pathologies. STR-MPs can be expanded and generate microglia-like cells in vitro, which we name stromal microglia (STR-M). STR-M cells show phagocytic ability, classically activate, and survive and phagocyte in human brain tissue. Thus, our results reveal that hBM harbors a source of microglia-like precursors that can be used in patient-centered fast derivative approaches.
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- author
- Bruzelius, Andreas LU ; Hidalgo, Isabel LU ; Boza-Serrano, Antonio LU ; Hjelmér, Anna Giorgia ; Tison, Amelie ; Deierborg, Tomas LU ; Bengzon, Johan LU and Ramos-Moreno, Tania LU
- organization
-
- Regenerative Neurophysiology (research group)
- StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
- MultiPark: Multidisciplinary research focused on Parkinson´s disease
- Division of Molecular Hematology (DMH)
- Experimental Dementia Research (research group)
- Neuroinflammation (research group)
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- bone marrow, common myeloid progenitor, human bone marrow, microglia, microglia-like cell in vitro model, microglial precursor, pluripotent stem cell, primitive myeloid progenitor
- in
- Stem cells translational medicine
- volume
- 10
- issue
- 4
- pages
- 582 - 597
- publisher
- AlphaMed Press
- external identifiers
-
- pmid:33295698
- scopus:85097313846
- ISSN
- 2157-6564
- DOI
- 10.1002/sctm.20-0127
- language
- English
- LU publication?
- yes
- id
- 0a176c4c-c1ab-4e35-adca-9383a471f659
- date added to LUP
- 2020-12-18 11:19:23
- date last changed
- 2024-09-19 11:49:43
@article{0a176c4c-c1ab-4e35-adca-9383a471f659, abstract = {{<p>Microglia, the immune sentinel of the central nervous system (CNS), are generated from yolk sac erythromyeloid progenitors that populate the developing CNS. Interestingly, a specific type of bone marrow-derived monocyte is able to express a yolk sac microglial signature and populate CNS in disease. Here we have examined human bone marrow (hBM) in an attempt to identify novel cell sources for generating microglia-like cells to use in cell-based therapies and in vitro modeling. We demonstrate that hBM stroma harbors a progenitor cell that we name stromal microglial progenitor (STR-MP). STR-MP single-cell gene analysis revealed the expression of the consensus genetic microglial signature and microglial-specific genes present in development and CNS pathologies. STR-MPs can be expanded and generate microglia-like cells in vitro, which we name stromal microglia (STR-M). STR-M cells show phagocytic ability, classically activate, and survive and phagocyte in human brain tissue. Thus, our results reveal that hBM harbors a source of microglia-like precursors that can be used in patient-centered fast derivative approaches.</p>}}, author = {{Bruzelius, Andreas and Hidalgo, Isabel and Boza-Serrano, Antonio and Hjelmér, Anna Giorgia and Tison, Amelie and Deierborg, Tomas and Bengzon, Johan and Ramos-Moreno, Tania}}, issn = {{2157-6564}}, keywords = {{bone marrow; common myeloid progenitor; human bone marrow; microglia; microglia-like cell in vitro model; microglial precursor; pluripotent stem cell; primitive myeloid progenitor}}, language = {{eng}}, number = {{4}}, pages = {{582--597}}, publisher = {{AlphaMed Press}}, series = {{Stem cells translational medicine}}, title = {{The human bone marrow harbors a CD45<sup>−</sup> CD11B<sup>+</sup> cell progenitor permitting rapid microglia-like cell derivative approaches}}, url = {{http://dx.doi.org/10.1002/sctm.20-0127}}, doi = {{10.1002/sctm.20-0127}}, volume = {{10}}, year = {{2021}}, }