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The human bone marrow harbors a CD45− CD11B+ cell progenitor permitting rapid microglia-like cell derivative approaches

Bruzelius, Andreas LU ; Hidalgo, Isabel LU orcid ; Boza-Serrano, Antonio LU ; Hjelmér, Anna Giorgia ; Tison, Amelie ; Deierborg, Tomas LU ; Bengzon, Johan LU and Ramos-Moreno, Tania LU orcid (2021) In Stem cells translational medicine 10(4). p.582-597
Abstract

Microglia, the immune sentinel of the central nervous system (CNS), are generated from yolk sac erythromyeloid progenitors that populate the developing CNS. Interestingly, a specific type of bone marrow-derived monocyte is able to express a yolk sac microglial signature and populate CNS in disease. Here we have examined human bone marrow (hBM) in an attempt to identify novel cell sources for generating microglia-like cells to use in cell-based therapies and in vitro modeling. We demonstrate that hBM stroma harbors a progenitor cell that we name stromal microglial progenitor (STR-MP). STR-MP single-cell gene analysis revealed the expression of the consensus genetic microglial signature and microglial-specific genes present in development... (More)

Microglia, the immune sentinel of the central nervous system (CNS), are generated from yolk sac erythromyeloid progenitors that populate the developing CNS. Interestingly, a specific type of bone marrow-derived monocyte is able to express a yolk sac microglial signature and populate CNS in disease. Here we have examined human bone marrow (hBM) in an attempt to identify novel cell sources for generating microglia-like cells to use in cell-based therapies and in vitro modeling. We demonstrate that hBM stroma harbors a progenitor cell that we name stromal microglial progenitor (STR-MP). STR-MP single-cell gene analysis revealed the expression of the consensus genetic microglial signature and microglial-specific genes present in development and CNS pathologies. STR-MPs can be expanded and generate microglia-like cells in vitro, which we name stromal microglia (STR-M). STR-M cells show phagocytic ability, classically activate, and survive and phagocyte in human brain tissue. Thus, our results reveal that hBM harbors a source of microglia-like precursors that can be used in patient-centered fast derivative approaches.

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bone marrow, common myeloid progenitor, human bone marrow, microglia, microglia-like cell in vitro model, microglial precursor, pluripotent stem cell, primitive myeloid progenitor
in
Stem cells translational medicine
volume
10
issue
4
pages
582 - 597
publisher
AlphaMed Press
external identifiers
  • scopus:85097313846
  • pmid:33295698
ISSN
2157-6564
DOI
10.1002/sctm.20-0127
language
English
LU publication?
yes
id
0a176c4c-c1ab-4e35-adca-9383a471f659
date added to LUP
2020-12-18 11:19:23
date last changed
2024-06-13 02:37:46
@article{0a176c4c-c1ab-4e35-adca-9383a471f659,
  abstract     = {{<p>Microglia, the immune sentinel of the central nervous system (CNS), are generated from yolk sac erythromyeloid progenitors that populate the developing CNS. Interestingly, a specific type of bone marrow-derived monocyte is able to express a yolk sac microglial signature and populate CNS in disease. Here we have examined human bone marrow (hBM) in an attempt to identify novel cell sources for generating microglia-like cells to use in cell-based therapies and in vitro modeling. We demonstrate that hBM stroma harbors a progenitor cell that we name stromal microglial progenitor (STR-MP). STR-MP single-cell gene analysis revealed the expression of the consensus genetic microglial signature and microglial-specific genes present in development and CNS pathologies. STR-MPs can be expanded and generate microglia-like cells in vitro, which we name stromal microglia (STR-M). STR-M cells show phagocytic ability, classically activate, and survive and phagocyte in human brain tissue. Thus, our results reveal that hBM harbors a source of microglia-like precursors that can be used in patient-centered fast derivative approaches.</p>}},
  author       = {{Bruzelius, Andreas and Hidalgo, Isabel and Boza-Serrano, Antonio and Hjelmér, Anna Giorgia and Tison, Amelie and Deierborg, Tomas and Bengzon, Johan and Ramos-Moreno, Tania}},
  issn         = {{2157-6564}},
  keywords     = {{bone marrow; common myeloid progenitor; human bone marrow; microglia; microglia-like cell in vitro model; microglial precursor; pluripotent stem cell; primitive myeloid progenitor}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{582--597}},
  publisher    = {{AlphaMed Press}},
  series       = {{Stem cells translational medicine}},
  title        = {{The human bone marrow harbors a CD45<sup>−</sup> CD11B<sup>+</sup> cell progenitor permitting rapid microglia-like cell derivative approaches}},
  url          = {{http://dx.doi.org/10.1002/sctm.20-0127}},
  doi          = {{10.1002/sctm.20-0127}},
  volume       = {{10}},
  year         = {{2021}},
}