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Presence of activated mobile fibroblasts in bronchoalveolar lavage from patients with mild asthma

Larsen, Kristoffer LU ; Tufvesson, Ellen LU ; Malmström, Johan LU orcid ; Mörgelin, Matthias LU ; Wildt, Marie LU ; Andersson Sjöland, Annika LU ; Lindström, Anna Lisa LU ; Malmström, Anders LU orcid ; Löfdahl, Claes-Göran LU and Marko-Varga, György LU , et al. (2004) In American Journal of Respiratory and Critical Care Medicine 170(10). p.1049-1056
Abstract
Activated fibroblasts are suggested to be involved in the deposition of extracellular matrix in the formation of peribronchial fibrosis in asthma. We report the novel finding of activated elongated fibroblasts accompanied by elevated numbers of eosinophils in bronchoalveolar lavage fluid from 5 out of 12 patients with mild asthma (= 42%), whereas no fibroblasts were observed in the control subjects without asthma (n = 17). The elongated fibroblasts migrated twice as far when compared with fibroblasts from corresponding bronchial biopsies from the same patients, accompanied by an induced expression of RhoA and Rac1, indicating that the increased expression of these proteins are linked to increased migratory capabilities. Moreover, the... (More)
Activated fibroblasts are suggested to be involved in the deposition of extracellular matrix in the formation of peribronchial fibrosis in asthma. We report the novel finding of activated elongated fibroblasts accompanied by elevated numbers of eosinophils in bronchoalveolar lavage fluid from 5 out of 12 patients with mild asthma (= 42%), whereas no fibroblasts were observed in the control subjects without asthma (n = 17). The elongated fibroblasts migrated twice as far when compared with fibroblasts from corresponding bronchial biopsies from the same patients, accompanied by an induced expression of RhoA and Rac1, indicating that the increased expression of these proteins are linked to increased migratory capabilities. Moreover, the elongated fibroblasts had an elevated production of the proteoglycans biglycan, versican, perlecan, and decorin, which correlated to an active cytoplasm in these cells. Differential expression patterns between the two fibroblast groups in motility-regulating proteins, such as cofilin, nuclear chloride ion channel protein, and heat-shock protein 20, were identified by two-dimensional electrophoresis and mass spectrometry. These findings indicate the presence of activated and mobile fibroblasts accompanied by an induced inflammatory response outside the airway epithelium in patients with mild asthma, results that may play a role in formation of airway fibrosis. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
American Journal of Respiratory and Critical Care Medicine
volume
170
issue
10
pages
1049 - 1056
publisher
American Thoracic Society
external identifiers
  • wos:000225076700004
  • pmid:15256392
  • scopus:8444250651
ISSN
1535-4970
DOI
10.1164/rccm.200404-507OC
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Matrix biology (013212025), Department of Experimental Medical Science (013210000), Division of Infection Medicine (BMC) (013024020), Respiratory Medicine and Allergology (013230111)
id
0b8451da-8388-495f-8433-dd7e9b46f9b2 (old id 138330)
date added to LUP
2016-04-01 11:56:55
date last changed
2024-01-23 00:15:25
@article{0b8451da-8388-495f-8433-dd7e9b46f9b2,
  abstract     = {{Activated fibroblasts are suggested to be involved in the deposition of extracellular matrix in the formation of peribronchial fibrosis in asthma. We report the novel finding of activated elongated fibroblasts accompanied by elevated numbers of eosinophils in bronchoalveolar lavage fluid from 5 out of 12 patients with mild asthma (= 42%), whereas no fibroblasts were observed in the control subjects without asthma (n = 17). The elongated fibroblasts migrated twice as far when compared with fibroblasts from corresponding bronchial biopsies from the same patients, accompanied by an induced expression of RhoA and Rac1, indicating that the increased expression of these proteins are linked to increased migratory capabilities. Moreover, the elongated fibroblasts had an elevated production of the proteoglycans biglycan, versican, perlecan, and decorin, which correlated to an active cytoplasm in these cells. Differential expression patterns between the two fibroblast groups in motility-regulating proteins, such as cofilin, nuclear chloride ion channel protein, and heat-shock protein 20, were identified by two-dimensional electrophoresis and mass spectrometry. These findings indicate the presence of activated and mobile fibroblasts accompanied by an induced inflammatory response outside the airway epithelium in patients with mild asthma, results that may play a role in formation of airway fibrosis.}},
  author       = {{Larsen, Kristoffer and Tufvesson, Ellen and Malmström, Johan and Mörgelin, Matthias and Wildt, Marie and Andersson Sjöland, Annika and Lindström, Anna Lisa and Malmström, Anders and Löfdahl, Claes-Göran and Marko-Varga, György and Bjermer, Leif and Westergren-Thorsson, Gunilla}},
  issn         = {{1535-4970}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{1049--1056}},
  publisher    = {{American Thoracic Society}},
  series       = {{American Journal of Respiratory and Critical Care Medicine}},
  title        = {{Presence of activated mobile fibroblasts in bronchoalveolar lavage from patients with mild asthma}},
  url          = {{http://dx.doi.org/10.1164/rccm.200404-507OC}},
  doi          = {{10.1164/rccm.200404-507OC}},
  volume       = {{170}},
  year         = {{2004}},
}