A 5' stem-loop and ribosome binding but not translation are important for the stability of Bacillus subtilis aprE leader mRNA.
(2002) In Microbiology 148(Pt 6). p.1795-1803- Abstract
- The Bacillus subtilis aprE leader is a determinant of extreme mRNA stability. The authors examined what properties of the aprE leader confer stability on an mRNA. The secondary structure of the aprE leader mRNA was analysed in vitro and in vivo, and mutations were introduced into different domains of an aprE leader-lacZ fusion. The half-lives of the corresponding transcripts were determined and beta-galactosidase activities were measured. Removal of a stem-loop structure at the 5' end or diminishing the strength of the RBS reduced the half-lives from more than 25 min to about 5 min. Interfering with translation by abolishing the start codon or creating an early stop codon had no or little effect on mRNA stability. The authors conclude that... (More)
- The Bacillus subtilis aprE leader is a determinant of extreme mRNA stability. The authors examined what properties of the aprE leader confer stability on an mRNA. The secondary structure of the aprE leader mRNA was analysed in vitro and in vivo, and mutations were introduced into different domains of an aprE leader-lacZ fusion. The half-lives of the corresponding transcripts were determined and beta-galactosidase activities were measured. Removal of a stem-loop structure at the 5' end or diminishing the strength of the RBS reduced the half-lives from more than 25 min to about 5 min. Interfering with translation by abolishing the start codon or creating an early stop codon had no or little effect on mRNA stability. The authors conclude that a 5' stem-loop and binding of ribosomes are necessary for the stability of aprE leader mRNA. The present results, together with a number of other data, suggest that translation of a B. subtilis mRNA is generally not important for its stability; the situation seems different in Escherichia coli. It is further concluded that the calculated strength of a B. subtilis RBS cannot be used to predict the stability of the corresponding transcript. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/108708
- author
- Hambraeus, Gustav LU ; Karhumaa, Kaisa LU and Rutberg, Blanka LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Microbiology
- volume
- 148
- issue
- Pt 6
- pages
- 1795 - 1803
- publisher
- MAIK Nauka/Interperiodica
- external identifiers
-
- pmid:12055299
- wos:000176304800020
- scopus:0035986640
- ISSN
- 1465-2080
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Applied Microbiology (LTH) (011001021), Biology building (Closed 2011) (011008000), Department of Cell and Organism Biology (Closed 2011.) (011002100)
- id
- 012275cd-1a51-4ced-82aa-75da7030d979 (old id 108708)
- alternative location
- http://mic.sgmjournals.org/cgi/content/abstract/148/6/1795
- date added to LUP
- 2016-04-01 12:30:10
- date last changed
- 2022-04-13 19:49:50
@article{012275cd-1a51-4ced-82aa-75da7030d979,
abstract = {{The Bacillus subtilis aprE leader is a determinant of extreme mRNA stability. The authors examined what properties of the aprE leader confer stability on an mRNA. The secondary structure of the aprE leader mRNA was analysed in vitro and in vivo, and mutations were introduced into different domains of an aprE leader-lacZ fusion. The half-lives of the corresponding transcripts were determined and beta-galactosidase activities were measured. Removal of a stem-loop structure at the 5' end or diminishing the strength of the RBS reduced the half-lives from more than 25 min to about 5 min. Interfering with translation by abolishing the start codon or creating an early stop codon had no or little effect on mRNA stability. The authors conclude that a 5' stem-loop and binding of ribosomes are necessary for the stability of aprE leader mRNA. The present results, together with a number of other data, suggest that translation of a B. subtilis mRNA is generally not important for its stability; the situation seems different in Escherichia coli. It is further concluded that the calculated strength of a B. subtilis RBS cannot be used to predict the stability of the corresponding transcript.}},
author = {{Hambraeus, Gustav and Karhumaa, Kaisa and Rutberg, Blanka}},
issn = {{1465-2080}},
language = {{eng}},
number = {{Pt 6}},
pages = {{1795--1803}},
publisher = {{MAIK Nauka/Interperiodica}},
series = {{Microbiology}},
title = {{A 5' stem-loop and ribosome binding but not translation are important for the stability of Bacillus subtilis aprE leader mRNA.}},
url = {{https://lup.lub.lu.se/search/files/2949389/623621.pdf}},
volume = {{148}},
year = {{2002}},
}