Oligosaccharide mapping of proteoglycan-bound and xyloside-initiated dermatan sulfate from fibroblasts
Fransson, Lars-Åke LU ; Schmidtchen, Artur LU ; Coster, Lars and Malmström, Anders LU
(1991)
In Glycoconjugate Journal
8(2).
p.108-115
- Abstract
- The copolymeric structure of dermatan sulfate chains synthesized by skin fibroblasts has been examined. Chains initiated onto exogeneous p-nitrophenyl-beta-D-xylopyranoside or attached to protein in a large proteoglycan, PG-L, and two small proteoglycans, PG-S1 and PG-S2, have been compared by using high resolution electrophoresis and gel chromatography of oligosaccharides generated by specific enzymatic or chemical degradations. The results confirm that chains attached to PG-L are glucuronate-rich, whereas novel findings indicate that chains attached to either of the two PG-S variants yield closely similar oligosaccharide maps, have approximately equal glucuronate and iduronate content and contain over 90% 4-sulfated disaccharide... (More)
- The copolymeric structure of dermatan sulfate chains synthesized by skin fibroblasts has been examined. Chains initiated onto exogeneous p-nitrophenyl-beta-D-xylopyranoside or attached to protein in a large proteoglycan, PG-L, and two small proteoglycans, PG-S1 and PG-S2, have been compared by using high resolution electrophoresis and gel chromatography of oligosaccharides generated by specific enzymatic or chemical degradations. The results confirm that chains attached to PG-L are glucuronate-rich, whereas novel findings indicate that chains attached to either of the two PG-S variants yield closely similar oligosaccharide maps, have approximately equal glucuronate and iduronate content and contain over 90% 4-sulfated disaccharide repeating units. Dermatan sulfate chains built onto xyloside at concentrations of 50 microM and below have a copolymeric structure similar to that of chains from the two PG-S variants. These findings indicate that the polymer-modifying machinery can generate chains with extended iduronate-containing repeats also when the xylose primer is not linked to core protein. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1106001
- author
- Fransson, Lars-Åke
LU
; Schmidtchen, Artur
LU
; Coster, Lars
and Malmström, Anders
LU
- organization
- publishing date
- 1991
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- oligosaccharide mapping, xylosides, dermatan sulfate, Proteoglycans
- in
- Glycoconjugate Journal
- volume
- 8
- issue
- 2
- pages
- 108 - 115
- publisher
- Springer
- external identifiers
-
- pmid:1823614
- scopus:0026331562
- ISSN
- 1573-4986
- DOI
- 10.1007/BF00731020
- language
- English
- LU publication?
- yes
- id
- ad2cfdbf-0599-47c3-9dcc-96f562e49240 (old id 1106001)
- date added to LUP
- 2016-04-01 17:08:55
- date last changed
- 2021-01-03 06:53:46
@article{ad2cfdbf-0599-47c3-9dcc-96f562e49240,
abstract = {{The copolymeric structure of dermatan sulfate chains synthesized by skin fibroblasts has been examined. Chains initiated onto exogeneous p-nitrophenyl-beta-D-xylopyranoside or attached to protein in a large proteoglycan, PG-L, and two small proteoglycans, PG-S1 and PG-S2, have been compared by using high resolution electrophoresis and gel chromatography of oligosaccharides generated by specific enzymatic or chemical degradations. The results confirm that chains attached to PG-L are glucuronate-rich, whereas novel findings indicate that chains attached to either of the two PG-S variants yield closely similar oligosaccharide maps, have approximately equal glucuronate and iduronate content and contain over 90% 4-sulfated disaccharide repeating units. Dermatan sulfate chains built onto xyloside at concentrations of 50 microM and below have a copolymeric structure similar to that of chains from the two PG-S variants. These findings indicate that the polymer-modifying machinery can generate chains with extended iduronate-containing repeats also when the xylose primer is not linked to core protein.}},
author = {{Fransson, Lars-Åke and Schmidtchen, Artur and Coster, Lars and Malmström, Anders}},
issn = {{1573-4986}},
keywords = {{oligosaccharide mapping; xylosides; dermatan sulfate; Proteoglycans}},
language = {{eng}},
number = {{2}},
pages = {{108--115}},
publisher = {{Springer}},
series = {{Glycoconjugate Journal}},
title = {{Oligosaccharide mapping of proteoglycan-bound and xyloside-initiated dermatan sulfate from fibroblasts}},
url = {{http://dx.doi.org/10.1007/BF00731020}},
doi = {{10.1007/BF00731020}},
volume = {{8}},
year = {{1991}},
}