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Oligosaccharide mapping of proteoglycan-bound and xyloside-initiated dermatan sulfate from fibroblasts

Fransson, Lars-Åke LU ; Schmidtchen, Artur LU ; Coster, Lars and Malmström, Anders LU (1991) In Glycoconjugate Journal 8(2). p.108-115
Abstract
The copolymeric structure of dermatan sulfate chains synthesized by skin fibroblasts has been examined. Chains initiated onto exogeneous p-nitrophenyl-beta-D-xylopyranoside or attached to protein in a large proteoglycan, PG-L, and two small proteoglycans, PG-S1 and PG-S2, have been compared by using high resolution electrophoresis and gel chromatography of oligosaccharides generated by specific enzymatic or chemical degradations. The results confirm that chains attached to PG-L are glucuronate-rich, whereas novel findings indicate that chains attached to either of the two PG-S variants yield closely similar oligosaccharide maps, have approximately equal glucuronate and iduronate content and contain over 90% 4-sulfated disaccharide... (More)
The copolymeric structure of dermatan sulfate chains synthesized by skin fibroblasts has been examined. Chains initiated onto exogeneous p-nitrophenyl-beta-D-xylopyranoside or attached to protein in a large proteoglycan, PG-L, and two small proteoglycans, PG-S1 and PG-S2, have been compared by using high resolution electrophoresis and gel chromatography of oligosaccharides generated by specific enzymatic or chemical degradations. The results confirm that chains attached to PG-L are glucuronate-rich, whereas novel findings indicate that chains attached to either of the two PG-S variants yield closely similar oligosaccharide maps, have approximately equal glucuronate and iduronate content and contain over 90% 4-sulfated disaccharide repeating units. Dermatan sulfate chains built onto xyloside at concentrations of 50 microM and below have a copolymeric structure similar to that of chains from the two PG-S variants. These findings indicate that the polymer-modifying machinery can generate chains with extended iduronate-containing repeats also when the xylose primer is not linked to core protein. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
oligosaccharide mapping, xylosides, dermatan sulfate, Proteoglycans
in
Glycoconjugate Journal
volume
8
issue
2
pages
108 - 115
publisher
Springer
external identifiers
  • pmid:1823614
  • scopus:0026331562
ISSN
1573-4986
DOI
10.1007/BF00731020
language
English
LU publication?
yes
id
ad2cfdbf-0599-47c3-9dcc-96f562e49240 (old id 1106001)
date added to LUP
2008-08-01 13:39:45
date last changed
2017-08-13 04:31:15
@article{ad2cfdbf-0599-47c3-9dcc-96f562e49240,
  abstract     = {The copolymeric structure of dermatan sulfate chains synthesized by skin fibroblasts has been examined. Chains initiated onto exogeneous p-nitrophenyl-beta-D-xylopyranoside or attached to protein in a large proteoglycan, PG-L, and two small proteoglycans, PG-S1 and PG-S2, have been compared by using high resolution electrophoresis and gel chromatography of oligosaccharides generated by specific enzymatic or chemical degradations. The results confirm that chains attached to PG-L are glucuronate-rich, whereas novel findings indicate that chains attached to either of the two PG-S variants yield closely similar oligosaccharide maps, have approximately equal glucuronate and iduronate content and contain over 90% 4-sulfated disaccharide repeating units. Dermatan sulfate chains built onto xyloside at concentrations of 50 microM and below have a copolymeric structure similar to that of chains from the two PG-S variants. These findings indicate that the polymer-modifying machinery can generate chains with extended iduronate-containing repeats also when the xylose primer is not linked to core protein.},
  author       = {Fransson, Lars-Åke and Schmidtchen, Artur and Coster, Lars and Malmström, Anders},
  issn         = {1573-4986},
  keyword      = {oligosaccharide mapping,xylosides,dermatan sulfate,Proteoglycans},
  language     = {eng},
  number       = {2},
  pages        = {108--115},
  publisher    = {Springer},
  series       = {Glycoconjugate Journal},
  title        = {Oligosaccharide mapping of proteoglycan-bound and xyloside-initiated dermatan sulfate from fibroblasts},
  url          = {http://dx.doi.org/10.1007/BF00731020},
  volume       = {8},
  year         = {1991},
}