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Platelet-derived growth factor promotes survival of rat and human mesencephalic dopaminergic neurons in culture

Nikkhah, G; Odin, Per LU ; Smits, A; Tingström, Anders LU ; Sanmartin, Agneta LU ; Brundin, Patrik LU ; Funa, K and Lindvall, Olle LU (1993) In Experimental Brain Research 92(3). p.516-523
Abstract
The effect of two isoforms of platelet-derived growth factor (PDGF), PDGF-AA and PDGF-BB, was tested on dissociated cell cultures of ventral mesencephalon from rat and human embryos. PDGF-BB but not PDGF-AA reduced the progressive loss of tyrosine hydroxylase- (TH)-positive neurons in rat and human cell cultures. The mean number of TH-positive cells in the PDGF-BB-treated rat culture was 64% and 106% higher than in the control cultures after 7 and 10 days in vitro, respectively. Corresponding figures for human TH-positive neurons were 90% and 145%. The influence of PDGF-BB was specific for TH-positive neurons and not a general trophic effect, since no change of either total cell number or metabolic activity was found. In PDGF-BB-treated... (More)
The effect of two isoforms of platelet-derived growth factor (PDGF), PDGF-AA and PDGF-BB, was tested on dissociated cell cultures of ventral mesencephalon from rat and human embryos. PDGF-BB but not PDGF-AA reduced the progressive loss of tyrosine hydroxylase- (TH)-positive neurons in rat and human cell cultures. The mean number of TH-positive cells in the PDGF-BB-treated rat culture was 64% and 106% higher than in the control cultures after 7 and 10 days in vitro, respectively. Corresponding figures for human TH-positive neurons were 90% and 145%. The influence of PDGF-BB was specific for TH-positive neurons and not a general trophic effect, since no change of either total cell number or metabolic activity was found. In PDGF-BB-treated cultures of human but not rat tissue the TH-positive neurons had longer neurites than observed in control or PDGF-AA-treated cultures. These data indicate that PDGF-BB may act as a trophic factor for mesencephalic dopaminergic neurons and suggest that administration of PDGF-BB could ameliorate degeneration and possibly promote axonal sprouting of these neurons in vivo. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Dopaminergic neurons, Platelet-derived growth factor, Cell culture, Parkinson's disease, Rat, Human
in
Experimental Brain Research
volume
92
issue
3
pages
516 - 523
publisher
Springer
external identifiers
  • pmid:8095907
  • scopus:0027082893
ISSN
0014-4819
DOI
10.1007/BF00229041
language
English
LU publication?
yes
id
b965dfa5-eda2-4ffc-814e-69b1fe285368 (old id 1107589)
date added to LUP
2008-07-31 13:04:59
date last changed
2017-08-06 03:35:44
@article{b965dfa5-eda2-4ffc-814e-69b1fe285368,
  abstract     = {The effect of two isoforms of platelet-derived growth factor (PDGF), PDGF-AA and PDGF-BB, was tested on dissociated cell cultures of ventral mesencephalon from rat and human embryos. PDGF-BB but not PDGF-AA reduced the progressive loss of tyrosine hydroxylase- (TH)-positive neurons in rat and human cell cultures. The mean number of TH-positive cells in the PDGF-BB-treated rat culture was 64% and 106% higher than in the control cultures after 7 and 10 days in vitro, respectively. Corresponding figures for human TH-positive neurons were 90% and 145%. The influence of PDGF-BB was specific for TH-positive neurons and not a general trophic effect, since no change of either total cell number or metabolic activity was found. In PDGF-BB-treated cultures of human but not rat tissue the TH-positive neurons had longer neurites than observed in control or PDGF-AA-treated cultures. These data indicate that PDGF-BB may act as a trophic factor for mesencephalic dopaminergic neurons and suggest that administration of PDGF-BB could ameliorate degeneration and possibly promote axonal sprouting of these neurons in vivo.},
  author       = {Nikkhah, G and Odin, Per and Smits, A and Tingström, Anders and Sanmartin, Agneta and Brundin, Patrik and Funa, K and Lindvall, Olle},
  issn         = {0014-4819},
  keyword      = {Dopaminergic neurons,Platelet-derived growth factor,Cell culture,Parkinson's disease,Rat,Human},
  language     = {eng},
  number       = {3},
  pages        = {516--523},
  publisher    = {Springer},
  series       = {Experimental Brain Research},
  title        = {Platelet-derived growth factor promotes survival of rat and human mesencephalic dopaminergic neurons in culture},
  url          = {http://dx.doi.org/10.1007/BF00229041},
  volume       = {92},
  year         = {1993},
}