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Regional brain-derived neurotrophic factor mRNA and protein levels following transient forebrain ischemia in the rat

Kokaia, Zaal LU ; Nawa, H; Uchino, H; Elmer, Eskil LU ; Kokaia, M; Carnahan, J; Smith, M L; Siesjo, B K and Lindvall, O (1996) In Brain Research. Molecular Brain Research 38(1). p.139-144
Abstract
Levels of BDNF mRNA and protein were measured in the rat brain using in situ hybridization and a two-site enzyme immunoassay. Under basal conditions, the highest BDNF concentration was found in the dentate gyrus (88 ng/g), while the levels in CA3 (50 ng/g), CA1 (18 ng/g) and parietal cortex (8 ng/g) were markedly lower. Following 10 min of forebrain ischemia, BDNF protein increased transiently in the dentate gyrus (to 124% of control at 6 h after the insult) and CA3 region (to 131% of control, at 1 week after the insult). In CA1 and parietal cortex, BDNF protein decreased to 73-75% of control at 24 h. In contrast, BDNF mRNA expression in dentate granule cells and CA3 pyramidal layer was transiently elevated to 287 and 293% of control,... (More)
Levels of BDNF mRNA and protein were measured in the rat brain using in situ hybridization and a two-site enzyme immunoassay. Under basal conditions, the highest BDNF concentration was found in the dentate gyrus (88 ng/g), while the levels in CA3 (50 ng/g), CA1 (18 ng/g) and parietal cortex (8 ng/g) were markedly lower. Following 10 min of forebrain ischemia, BDNF protein increased transiently in the dentate gyrus (to 124% of control at 6 h after the insult) and CA3 region (to 131% of control, at 1 week after the insult). In CA1 and parietal cortex, BDNF protein decreased to 73-75% of control at 24 h. In contrast, BDNF mRNA expression in dentate granule cells and CA3 pyramidal layer was transiently elevated to 287 and 293% of control, respectively, at 2 h, whereas no change was detected in CA1 or neocortex. The regional BDNF protein levels shown here correlate at least partly with regional differences in cellular resistance to ischemic damage, which is consistent with the hypothesis of a neuroprotective role of BDNF. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Rat, Brain damage, Hippocampus, Neurotrophins, Forebrain ischemia
in
Brain Research. Molecular Brain Research
volume
38
issue
1
pages
139 - 144
publisher
Elsevier
external identifiers
  • pmid:8737677
  • scopus:0029896172
ISSN
0169-328X
DOI
10.1016/0169-328X(96)00002-2
language
English
LU publication?
yes
id
6a462162-405a-4142-930b-8dea68f52dcb (old id 1110534)
date added to LUP
2008-07-25 14:08:35
date last changed
2017-08-13 04:30:35
@article{6a462162-405a-4142-930b-8dea68f52dcb,
  abstract     = {Levels of BDNF mRNA and protein were measured in the rat brain using in situ hybridization and a two-site enzyme immunoassay. Under basal conditions, the highest BDNF concentration was found in the dentate gyrus (88 ng/g), while the levels in CA3 (50 ng/g), CA1 (18 ng/g) and parietal cortex (8 ng/g) were markedly lower. Following 10 min of forebrain ischemia, BDNF protein increased transiently in the dentate gyrus (to 124% of control at 6 h after the insult) and CA3 region (to 131% of control, at 1 week after the insult). In CA1 and parietal cortex, BDNF protein decreased to 73-75% of control at 24 h. In contrast, BDNF mRNA expression in dentate granule cells and CA3 pyramidal layer was transiently elevated to 287 and 293% of control, respectively, at 2 h, whereas no change was detected in CA1 or neocortex. The regional BDNF protein levels shown here correlate at least partly with regional differences in cellular resistance to ischemic damage, which is consistent with the hypothesis of a neuroprotective role of BDNF.},
  author       = {Kokaia, Zaal and Nawa, H and Uchino, H and Elmer, Eskil and Kokaia, M and Carnahan, J and Smith, M L and Siesjo, B K and Lindvall, O},
  issn         = {0169-328X},
  keyword      = {Rat,Brain damage,Hippocampus,Neurotrophins,Forebrain ischemia},
  language     = {eng},
  number       = {1},
  pages        = {139--144},
  publisher    = {Elsevier},
  series       = {Brain Research. Molecular Brain Research},
  title        = {Regional brain-derived neurotrophic factor mRNA and protein levels following transient forebrain ischemia in the rat},
  url          = {http://dx.doi.org/10.1016/0169-328X(96)00002-2},
  volume       = {38},
  year         = {1996},
}